|
|
 Previous Article | Table of Contents | Next Article 
Development of human megakaryocytes: I. Hematopoietic progenitors (CD34+
bone marrow cells) are enriched with megakaryocytes expressing CD4
A Dolzhanskiy, RS Basch and S Karpatkin
Department of Pathology, New York University Medical Center, New York
10016, USA.
CD34 is expressed by essentially all human hematopoietic progenitors
including cells of the megakaryocyte (MK) lineage. We have previously
reported CD4 expression by some human MK (Blood 81:2,664, 1993). To study
the role of maturation on CD4 expression by MK, we examined CD34+ bone
marrow cells for their expression of CD41 (GPIIb-GPIIIa) and CD4 with
specific monoclonal antibody (MoAb)-fluorochrome conjugates and for DNA
polyploidization with propidium iodide or 7-aminoactinomycin D (7-AAD).
Surprisingly, MK were at least 20-fold more common in the CD34+ progenitor
pool (approximately 10%) than in the more mature CD34+ population
(approximately 0.5%) of low density bone marrow cells. CD4 expression
correlated with markers of immaturity in that CD4 was enriched among CD34+
cells, and the proportion of CD4+ MK declined with increasing ploidy.
Almost all CD34+ polyploid ( > or = 8N) cells were CD4+. Despite these
correlations with immaturity, CD34+CD4+ MK precursors were unable to
produce MK colony-forming units (CFU-MK) when cultured under conditions
that supported the growth of CFU-MK from CD34+CD4- MK lineage cells. MK
became polyploid before the loss of either CD34 or CD4 expression. The
presence of CD4 on these cells correlates with the onset of endomitotic
reduplication and is associated with the loss of the ability of these cells
to undergo normal mitotic division. The role of CD4 on immature MK as a
differentiation antigen and/or receptor for the human immunodeficiency
virus (HIV)-1 virus remains to be determined.
Volume 87,
Issue 4,
pp. 1353-1360,
02/15/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. Guerriero, I. Parolini, U. Testa, P. Samoggia, E. Petrucci, M. Sargiacomo, C. Chelucci, M. Gabbianelli, and C. Peschle
Inhibition of TPO-induced MEK or mTOR activity induces opposite effects on the ploidy of human differentiating megakaryocytes
J. Cell Sci.,
February 15, 2006;
119(4):
744 - 752.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Casella, T. Feccia, C. Chelucci, P. Samoggia, G. Castelli, R. Guerriero, I. Parolini, E. Petrucci, E. Pelosi, O. Morsilli, et al.
Autocrine-paracrine VEGF loops potentiate the maturation of megakaryocytic precursors through Flt1 receptor
Blood,
February 15, 2003;
101(4):
1316 - 1323.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Guerriero, G. Mattia, U. Testa, C. Chelucci, G. Macioce, I. Casella, P. Samoggia, C. Peschle, and H. J. Hassan
Stromal cell-derived factor 1{alpha} increases polyploidization of megakaryocytes generated by human hematopoietic progenitor cells
Blood,
May 1, 2001;
97(9):
2587 - 2595.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-Q. Huang, J.-J. Li, and S. Karpatkin
Identification of a family of alternatively spliced mRNA species of angiopoietin-1
Blood,
March 15, 2000;
95(6):
1993 - 1999.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Riviere, F. Subra, K. Cohen-Solal, V. Cordette-Lagarde, R. Letestu, C. Auclair, W. Vainchenker, and F. Louache
Phenotypic and Functional Evidence for the Expression of CXCR4 Receptor During Megakaryocytopoiesis
Blood,
March 1, 1999;
93(5):
1511 - 1523.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Poujol, D. T.-L. Roux, P. Tropel, V. Roullot, A. Nurden, G. Marguerie, and P. Nurden
Ultrastructural Analysis of Bone Marrow Hematopoiesis in Mice Transgenic for the Thymidine Kinase Gene Driven by the alpha IIb Promoter
Blood,
September 15, 1998;
92(6):
2012 - 2023.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. F. Weichold, D. Zella, O. Barabitskaja, J. P. Maciejewski, D. E. Dunn, E. M. Sloand, and N. S. Young
Neither Human Immunodeficiency Virus-1 (HIV-1) nor HIV-2 Infects Most-Primitive Human Hematopoietic Stem Cells as Assessed in Long-Term Bone Marrow Cultures
Blood,
February 1, 1998;
91(3):
907 - 915.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Bertolini, M. Battaglia, P. Pedrazzoli, G. Antonio Da Prada, A. Lanza, D. Soligo, L. Caneva, B. Sarina, S. Murphy, T. Thomas, et al.
Megakaryocytic Progenitors Can Be Generated Ex Vivo and Safely Administered to Autologous Peripheral Blood Progenitor Cell Transplant Recipients
Blood,
April 15, 1997;
89(8):
2679 - 2688.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Zauli, A. Bassini, M. Vitale, D. Gibellini, C. Celeghini, E. Caramelli, S. Pierpaoli, L. Guidotti, and S. Capitani
Thrombopoietin Enhances the alpha IIbbeta 3-Dependent Adhesion of Megakaryocytic Cells to Fibrinogen or Fibronectin Through PI 3 Kinase
Blood,
February 1, 1997;
89(3):
883 - 895.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
