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The synthesis and localization of alternatively spliced fibronectin EIIIB
in resting and thrombin-treated megakaryocytes
PK Schick, CM Wojenski, VD Bennett and T Ivanova
Cardeza Foundation for Hematologic Research, Thomas Jefferson University,
Philadelphia, PA, USA.
There are several species of alternatively spliced fibronectin (FN). One of
these, FN EIIIB, is primarily present in embryonic and in proliferating and
migrating cells and is believed to be important for cell maturation. We
have studied the synthesis, localization, and secretion of this FN isoform
in isolated guinea pig megakaryocytes, nonmegakaryocytic bone marrow cells,
and platelets. There was 7.5 times more general FN in megakaryocytes than
in nonmegakaryocytic cells based on the analysis of equivalent amounts of
protein. FN EIIIB was detected by Western blotting in megakaryocytes but
not in nonmegakaryocytic cells present in bone marrow. Neither
megakaryocytes nor platelets secreted FN EIIIB, while megakaryocytes
secreted 25.3% +/- 4.6% general FN and platelets secreted about 61% general
FN in response to thrombin. Analysis of immunostained cells by confocal
microscopy revealed that FN EIIIB had been redistributed to the surface of
megakaryocytes in response to thrombin. Synthesis was studied by metabolic
labeling, and megakaryocytes were shown to synthesize FN and FN EIIIB.
Thus, megakaryocytes and platelets are among a small number of adult cells
and tissues that synthesize and contain FN EIIIB. The expression of FN
EIIIB on the megakaryocyte surface may influence migration and maturation.
Volume 87,
Issue 5,
pp. 1817-1823,
03/01/1996
Copyright © 1996 by The American Society of Hematology
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