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The Tie receptor tyrosine kinase is expressed by human hematopoietic
progenitor cells and by a subset of megakaryocytic cells
P Batard, P Sansilvestri, C Scheinecker, W Knapp, N Debili, W Vainchenker, HJ Buhring, MN Monier, E Kukk, J Partanen, MT Matikainen, R Alitalo, J Hatzfeld and K Alitalo
Laboratoire de biologie molecularie et cellulaire des facteurs de
croissance, UPR 9044/CNRS, Villejuif, France.
Growth factor receptors in human hematopoietic progenitor cells have become
the focus of intense interest, because they may provide tools for the
monitoring, enrichment, and expansion of stem cells. We have shown earlier
that the Tie receptor tyrosine kinase is expressed in erythroid and
megakaryoblastic human leukemia cell lines, in the blood islands of the
yolk sac, and in endothelial cells starting from day 8.0 of mouse
development. Here, the expression of Tie was studied in human hematopoietic
cells of various sources. Peripheral blood mononuclear cells were Tie-.
However, a large fraction of CD34+ cells from umbilical cord blood (UCB)
and bone marrow (BM) expressed tie protein and mRNA. On average, 64% of the
fluorescence-activated cell sorting- gated UCB CD34+ cells including CD38-
cells and a fraction of cells expressing low levels of c-Kit were Tie+.
Also, 30% to 60% of BM CD34+ cells were Tie+, including most of the BM
CD34+CD38-, CD34+Thy-1+, and CD34+HLA-DR- cells. Under culture conditions
allowing myeloid, erythroid, and/or megakaryocytic differentiation,
purified UCB CD34+ cells lost Tie mRNA and protein expression concomitantly
with that of CD34; however, a significant fraction of cells expressed Tie
during megakaryocytic differentiation. These data suggest that, in humans,
the Tie receptor and presumably its ligand may function at an early stage
of hematopoietic cell differentiation.
Volume 87,
Issue 6,
pp. 2212-2220,
03/15/1996
Copyright © 1996 by The American Society of Hematology
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