|
|
 Previous Article | Table of Contents | Next Article 
Incidence and characterization of MLL gene (11q23) rearrangements in acute
myeloid leukemia M1 and M5
H Poirel, K Rack, E Delabesse, I Radford-Weiss, X Troussard, C Debert, D Leboeuf, C Bastard, F Picard, A Veil-Buzyn, G Flandrin, O Bernard and E Macintyre
Department of Haematology, University Paris V, France.
To determine the incidence of MLL rearrangement in acute myeloid leukemia
(AML) French-American-British (FAB) type M1 and to evaluate optimal
screening strategies for the characterization of such abnormalities, we
analyzed specimens from 41 patients with AML by Southern blotting with two
MLL genomic probes and compared the capacities of reverse
transcription-polymerase chain reaction (RT-PCR) and fluorescent in situ
hybridization (FISH) to identify the types of rearrangement found in AML M1
with those observed in AML M5. MLL rearrangement was found in 6 of 29 (20%)
AML M1 and 6 of 10 AML M5 cases. RT-PCR characterization of 11 cases showed
four MLL self- fusions, four MLL-AF6, two MLL-AF9, including a novel AF9
breakpoint, and one uncharacterized t(11:19). Only 5 of 10 MLL-rearranged
cases tested demonstrated karyotypic 11q23 abnormalities. FISH analysis of
nine cases with an MLL-specific yeast artificial chromosome (YAC) confirmed
the cytogenetic abnormalities in two cases, clarified them in one, and did
not detect six cases, including three MLL self-fusions, one case with a
probable MLL-rearranged subclone not represented karyotypically, and
twoMLL-AF6. A whole chromosome 11 paint detected one of these MLL-AF6, and
an AF6 cosmid demonstrated that the other was probably due to insertion of
a submicroscopic portion of chromosome 6, including part of AF6, into an
apparently normal chromosome 11. We conclude that MLL rearrangements are
common in adult AML M1, that MLL self-fusion and MLL-AF6 are the most
frequent types of abnormalities, and that RT-PCR is preferable to 11q23
FISH analysis for their characterization.
Volume 87,
Issue 6,
pp. 2496-2505,
03/15/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
N. Cerveira, F. Micci, J. Santos, M. Pinheiro, C. Correia, S. Lisboa, S. Bizarro, L. Norton, A. Glomstein, A. E. Asberg, et al.
Molecular characterization of the MLL-SEPT6 fusion gene in acute myeloid leukemia: identification of novel fusion transcripts and cloning of genomic breakpoint junctions
Haematologica,
July 1, 2008;
93(7):
1076 - 1080.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Libura, V. Asnafi, A. Tu, E. Delabesse, I. Tigaud, F. Cymbalista, A. Bennaceur-Griscelli, P. Villarese, G. Solbu, A. Hagemeijer, et al.
FLT3 and MLL intragenic abnormalities in AML reflect a common category of genotoxic stress
Blood,
September 15, 2003;
102(6):
2198 - 2204.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Oguchi, M. Takagi, R. Tsuchida, Y. Taya, E. Ito, K. Isoyama, E. Ishii, L. Zannini, D. Delia, and S. Mizutani
Missense mutation and defective function of ATM in a childhood acute leukemia patient with MLL gene rearrangement
Blood,
May 1, 2003;
101(9):
3622 - 3627.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Schnittger, C. Schoch, M. Dugas, W. Kern, P. Staib, C. Wuchter, H. Loffler, C. M. Sauerland, H. Serve, T. Buchner, et al.
Analysis of FLT3 length mutations in 1003 patients with acute myeloid leukemia: correlation to cytogenetics, FAB subtype, and prognosis in the AMLCG study and usefulness as a marker for the detection of minimal residual disease
Blood,
June 17, 2002;
100(1):
59 - 66.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Brumpt, E. Delabesse, K. Beldjord, F. Davi, J.-M. Cayuela, C. Millien, P. Villarese, P. Quartier, A. Buzyn, F. Valensi, et al.
The incidence of clonal T-cell receptor rearrangements in B-cell precursor acute lymphoblastic leukemia varies with age and genotype
Blood,
September 15, 2000;
96(6):
2254 - 2261.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Schnittger, B. Wormann, W. Hiddemann, and F. Griesinger
Partial Tandem Duplications of the MLL Gene Are Detectable in Peripheral Blood and Bone Marrow of Nearly All Healthy Donors
Blood,
September 1, 1998;
92(5):
1728 - 1734.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Pallisgaard, P. Hokland, D. C. Riishoj, B. Pedersen, and P. Jorgensen
Multiplex Reverse Transcription-Polymerase Chain Reaction for Simultaneous Screening of 29 Translocations and Chromosomal Aberrations in Acute Leukemia
Blood,
July 15, 1998;
92(2):
574 - 588.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.H. Dreyling, K. Schrader, C. Fonatsch, B. Schlegelberger, D. Haase, C. Schoch, W.-D. Ludwig, H. Loffler, T. Buchner, B. Wormann, et al.
MLL and CALM Are Fused to AF10 in Morphologically Distinct Subsets of Acute Leukemia With Translocation t(10;11): Both Rearrangements Are Associated With a Poor Prognosis
Blood,
June 15, 1998;
91(12):
4662 - 4667.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. P. Strout, G. Marcucci, C. D. Bloomfield, and M. A. Caligiuri
The partial tandem duplication of ALL1 (MLL) is consistently generated by Alu-mediated homologous recombination in acute myeloid leukemia
PNAS,
March 3, 1998;
95(5):
2390 - 2395.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Hess, B. D. Yu, B. Li, R. Hanson, and S. J. Korsmeyer
Defects in Yolk Sac Hematopoiesis in Mll-Null Embryos
Blood,
September 1, 1997;
90(5):
1799 - 1806.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. W. So, C. Caldas, M.-M. Liu, S.-J. Chen, Q.-H. Huang, L.-J. Gu, M. H. Sham, L. M. Wiedemann, and L. C. Chan
EEN encodes for a member of a new family of proteins containing an Src homology 3 domain and is the third gene located on chromosome 19p13 that fuses to MLL in human leukemia
PNAS,
March 18, 1997;
94(6):
2563 - 2568.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Macintyre, P. Bourquelot, D. Leboeuf, R. Rimokh, E. Archimbaud, T. Smetsers, and R. Zittoun
MLL Cleavage Occurs in Approximately 5% of De Novo Acute Myeloid Leukemia, Including in Patients Analyzed Before Treatment Induction
Blood,
March 15, 1997;
89(6):
2224 - 2225.
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Caslini, A. Shilatifard, L. Yang, and J. L. Hess
The amino terminus of the mixed lineage leukemia protein (MLL) promotes cell cycle arrest and monocytic differentiation
PNAS,
March 14, 2000;
97(6):
2797 - 2802.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
