|
|
 Previous Article | Table of Contents | Next Article 
Aplastic anemia: evidence for dysfunctional bone marrow progenitor cells
and the corrective effect of granulocyte colony-stimulating factor in vitro
J Scopes, S Daly, R Atkinson, SE Ball, EC Gordon-Smith and FM Gibson
Department of Cellular and Molecular Sciences, St. George's Hospital
Medical School, London, UK.
We investigated the effects of granulocyte-macrophage colony- stimulating
factor, interleukin-3, stem cell factor, interleukin-6, and granulocyte
colony-stimulating factor (G-CSF) alone, and in combination, on the
clonogenic potential of normal and aplastic anemia (AA) bone marrow
mononuclear cells (BMMC and CD34+ cells. AA BMMC consistently produced a
significantly lower absolute number of colonies than normal, but, when
account was taken of the reduced proportion of CD34+ cells in AA BM, there
was no significant difference in terms of cloning efficiency (CE). However,
when removed from the influence of accessory cells, the CE of AA CD34+
cells decreased significantly more than normal, indicating a defect in
their function, either in terms of dependence on accessory cell-derived
factors or susceptibility to cell damage when sorted. Of the factors
studied, G-CSF had the most significant effect on the response of CD34+
cells from both groups when removed from their accessory cells. This was
particularly true for AA CD34+ cells, whose response to cytokine stimuli
containing G-CSF enabled them to match the response of normal CD34+ cells.
Volume 87,
Issue 8,
pp. 3179-3185,
04/15/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Teramura, A. Kimura, S. Iwase, Y. Yonemura, S. Nakao, A. Urabe, M. Omine, and H. Mizoguchi
Treatment of severe aplastic anemia with antithymocyte globulin and cyclosporin A with or without G-CSF in adults: a multicenter randomized study in Japan
Blood,
September 15, 2007;
110(6):
1756 - 1761.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Wunschmann, B. Becker, and A. Vallbracht
Hepatitis A Virus Suppresses Monocyte-to-Macrophage Maturation In Vitro
J. Virol.,
March 27, 2002;
76(9):
4350 - 4356.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Podesta, G. Piaggio, F. Frassoni, A. Pitto, P. Zikos, M. Sessarego, M. Abate, M. Teresa Van Lint, G. Berisso, and A. Bacigalupo
The Assessment of the Hematopoietic Reservoir After Immunosuppressive Therapy or Bone Marrow Transplantation in Severe Aplastic Anemia
Blood,
March 15, 1998;
91(6):
1959 - 1965.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
