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A novel hematopoietic multilineage clone, Myl-D-7, is stromal cell-
dependent and supported by an alternative mechanism(s) independent of stem
cell factor/c-kit interaction
K Itoh, J Friel, N Kluge, T Kina, A Kondo-Takaori, S Kawamata, T Uchiyama and W Ostertag
Heinrich-Pette-Institute for Experimental Virology and Immunology, Hamburg
University, Germany.
A strictly stroma-dependent hematopoietic clone, Myl-D-7, with lympho-
myeloid potential has been isolated. A subset of cells expresses
myeloid-macrophage (Mac-1 and Gr-1), erythroid (TER119), and lymphoid
(Thy-1 and B220) lineage markers. Spontaneous differentiation to the
myeloid-macrophage, erythroid, or lymphoid pathway can be seen by
morphologic criteria, detection of beta major globin synthesis, or
expression of the early lymphoid specific transcription factor, Ikaros. By
sorting lineage marker (Mac-1, Gr-1, B220, and TER119)-negative (LIN- )
cells, we showed that the LIN- population actively self-renews on top of
MS-5 stromal cells, and differentiates to LIN+ cells. Removal of stroma
induces apoptosis and none of the growth factors tested can prevent
apoptosis. Granulocyte-macrophage colony-stimulating factor accelerates the
differentiation towards the myeloid-macrophage lineage. Using this clone,
we show that (1) contact with stroma induces expression of bcl-2, (2)
stromal cells derived from SI/SI homozygous fetuses can support long-term
growth, and (3) conditioned media of specific stromal cells contains an
activity that supports proliferation and self-renewal of the clone. Myl-D-7
can thus be used as an indicator cell for unknown factors that may provide
stromal cell support.
Volume 87,
Issue 8,
pp. 3218-3228,
04/15/1996
Copyright © 1996 by The American Society of Hematology
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