|
|
 Previous Article | Table of Contents | Next Article 
Exposure of human CD34+ cells to human immunodeficiency virus type 1 does
not influence their expansion and proliferation of hematopoietic
progenitors in vitro
S Kaushal, VF La Russa, S Gartner, S Kessler, S Perfetto, Z Yu, DW Ritchey, J Xu, P Perera, J Kim, T Reid, DL Mayers, D St. Louis and JD Mosca
Department of Hematology and Vascular Biology, Walter Reed Army Institute
of Research, Washington, DC; USA.
The susceptibility of highly purified human CD34+ cells to monocytotropic
(Ba-L) and lymphotropic (A018-post) strains of human immunodeficiency
virus-1 (HIV-1) was examined. Liquid cultures initiated with fresh
immunomagnetically purified CD34+ cells using the K6.1 CD34 monoclonal
antibody (MoAb) (K6.1/CD34+) were positive for HIV expression 2 weeks after
exposure to HIV-1 Ba-L. These cells were initially greater than 90% CD34+
and had undetectable monocyte contamination by flow-cytometric staining and
side-scatter analyses, respectively, and undetectable T-cell contamination
by CD3 polymerase chain reaction (PCR) analysis. However, secondary CD34+
liquid cultures reselected from the primary liquid cultures 24 hours after
HIV exposure by panning with the ICH3 CD34 MoAb (ICH3/CD34+) and maintained
for an additional 14 days were negative for HIV expression. The
ICH3-unbound cells were positive for both spliced and unspliced HIV RNA
when exposed to HIV-1 Ba-L, and were DNA PCR positive when exposed to
either monocytotropic or lymphotropic HIV-1. To further test that CD34+
cells were not infectible by HIV-1, we exposed K6.1/CD34+ cells
continuously to HIV-1 in a culture system capable of maintaining and
expanding primitive CD34+ cells. HIV-exposed K6.1/CD34+ cells proliferated
and expanded as efficiently as uninfected cultures. However, when
reselected magnetically using the K6.1 CD34 MoAb after expansion for 7
days, bound K6.1/CD34+ cells were again negative for HIV-1 expression,
whereas unbound cells were positive for HIV-1 expression. These findings
suggest that a sequential CD34+ cell-selection process, in which the two
selections are separated by a brief culture period, can yield a population
of CD34+ cells that are not infected with HIV-1. This process may be useful
in the design of stem or progenitor cell- based transplantation therapies
for HIV infection.
Volume 88,
Issue 1,
pp. 130-137,
07/01/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K. Imrie, R. Esmail, R. M. Meyer, and and the Members of the Hematology Disease Site Gro
The Role of High-Dose Chemotherapy and Stem-Cell Transplantation in Patients with Multiple Myeloma: A Practice Guideline of the Cancer Care Ontario Practice Guidelines Initiative
Ann Intern Med,
April 16, 2002;
136(8):
619 - 629.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ishii, M. Nishihara, F. Ma, Y. Ebihara, K. Tsuji, S. Asano, T. Nakahata, and T. Maekawa
Expression of Stromal Cell-Derived Factor-1/Pre-B Cell Growth-Stimulating Factor Receptor, CXC Chemokine Receptor 4, on CD34+ Human Bone Marrow Cells Is a Phenotypic Alteration for Committed Lymphoid Progenitors
J. Immunol.,
October 1, 1999;
163(7):
3612 - 3620.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Aiuti, L. Turchetto, M. Cota, A. Cipponi, A. Brambilla, C. Arcelloni, R. Paroni, E. Vicenzi, C. Bordignon, and G. Poli
Human CD34+ Cells Express CXCR4 and Its Ligand Stromal Cell-Derived Factor-1. Implications for Infection by T-Cell Tropic Human Immunodeficiency Virus
Blood,
July 1, 1999;
94(1):
62 - 73.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Mohle, F. Bautz, S. Rafii, M. A.S. Moore, W. Brugger, and L. Kanz
The Chemokine Receptor CXCR-4 Is Expressed on CD34+ Hematopoietic Progenitors and Leukemic Cells and Mediates Transendothelial Migration Induced by Stromal Cell-Derived Factor-1
Blood,
June 15, 1998;
91(12):
4523 - 4530.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Jenkins, M. B. Hanley, M. B. Moreno, E. Wieder, and J. M. McCune
Human Immunodeficiency Virus-1 Infection Interrupts Thymopoiesis and Multilineage Hematopoiesis In Vivo
Blood,
April 15, 1998;
91(8):
2672 - 2678.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Moses, J. Nelson, and G. C. Bagby Jr
The Influence of Human Immunodeficiency Virus-1 on Hematopoiesis
Blood,
March 1, 1998;
91(5):
1479 - 1495.
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Junker, J. J. Moon, C. S. Kalfoglou, I. Sniecinski, S. J. Forman, J. A. Zaia, H. Kaneshima, and E. Bohnlein
Hematopoietic Potential and Retroviral Transduction of CD34+Thy-1+ Peripheral Blood Stem Cells From Asymptomatic Human Immunodeficiency Virus Type-1-Infected Individuals Mobilized With Granulocyte Colony-Stimulating Factor
Blood,
June 15, 1997;
89(12):
4299 - 4306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
