|
|
 Previous Article | Table of Contents | Next Article 
Changes in cell spreading and cytoskeletal organization are induced by
adhesion to a fibronectin-fibrin matrix
SA Corbett, CL Wilson and JE Schwarzbauer
Department of Molecular Biology, Princeton University NJ; 08544-1014, USA.
Plasma fibronectin (pFN) cross-linked to fibrin during the injury response
provides a provisional matrix required for cells to begin tissue repair.
Using a synthetic matrix of pFN and fibrin as a substrate for cell adhesion
and spreading, we have determined that pFN covalently cross-linked to
fibrin into a complex multimer is functionally distinct from pFN
immobilized onto a plastic surface. NIH- 3T3 cells on a FN-fibrin matrix
reach 50% of the maximal cell area of cells spread on FN-coated plastic.
They neither attach nor spread on cross-linked fibrin alone. Cells on
pFN-fibrin matrices form few prominent stress fibers and exhibit clear
differences in membrane ruffling and filopodial extension when stained with
rhodamine-labeled phalloidin. Interestingly, these differences are enhanced
by upregulation of protein kinase C. These data suggest that cell-FN
interactions can be modified by the molecular context of the protein within
the extracellular matrix resulting in distinct cell morphology and
cytoskeletal organization.
Volume 88,
Issue 1,
pp. 158-166,
07/01/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Cho and D. F. Mosher
Enhancement of thrombogenesis by plasma fibronectin cross-linked to fibrin and assembled in platelet thrombi
Blood,
May 1, 2006;
107(9):
3555 - 3563.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cho, J. L. Degen, B. S. Coller, and D. F. Mosher
Fibrin but Not Adsorbed Fibrinogen Supports Fibronectin Assembly by Spread Platelets: EFFECTS OF THE INTERACTION OF {alpha}IIb{beta}3 WITH THE C TERMINUS OF THE FIBRINOGEN {gamma}-CHAIN
J. Biol. Chem.,
October 21, 2005;
280(42):
35490 - 35498.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. S. Midwood, L. V. Valenick, H. C. Hsia, and J. E. Schwarzbauer
Coregulation of Fibronectin Signaling and Matrix Contraction by Tenascin-C and Syndecan-4
Mol. Biol. Cell,
December 1, 2004;
15(12):
5670 - 5677.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O Sanchez-Pernaute, R Largo, E Calvo, M A Alvarez-Soria, J Egido, and G Herrero-Beaumont
A fibrin based model for rheumatoid synovitis
Ann Rheum Dis,
December 1, 2003;
62(12):
1135 - 1138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Sanchez-Pernaute, M. J. Lopez-Armada, E. Calvo, I. Diez-Ortego, R. Largo, J. Egido, and G. Herrero-Beaumont
Fibrin generated in the synovial fluid activates intimal cells from their apical surface: a sequential morphological study in antigen-induced arthritis
Rheumatology,
January 1, 2003;
42(1):
19 - 25.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. S. Midwood and J. E. Schwarzbauer
Tenascin-C Modulates Matrix Contraction via Focal Adhesion Kinase- and Rho-mediated Signaling Pathways
Mol. Biol. Cell,
October 1, 2002;
13(10):
3601 - 3613.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. B. Hotary, I. Yana, F. Sabeh, X.-Y. Li, K. Holmbeck, H. Birkedal-Hansen, E. D. Allen, N. Hiraoka, and S. J. Weiss
Matrix Metalloproteinases (MMPs) Regulate Fibrin-invasive Activity via MT1-MMP-dependent and -independent Processes
J. Exp. Med.,
January 28, 2002;
195(3):
295 - 308.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Pereira, B. J. Rybarczyk, T. M. Odrljin, D. C. Hocking, J. Sottile, and P. J. Simpson-Haidaris
The incorporation of fibrinogen into extracellular matrix is dependent on active assembly of a fibronectin matrix
J. Cell Sci.,
January 2, 2002;
115(3):
609 - 617.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. B. Wenk, K. S. Midwood, and J. E. Schwarzbauer
Tenascin-C Suppresses Rho Activation
J. Cell Biol.,
August 21, 2000;
150(4):
913 - 920.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Corbett and J. E. Schwarzbauer
Requirements for alpha 5beta 1 Integrin-mediated Retraction of Fibronectin-Fibrin Matrices
J. Biol. Chem.,
July 23, 1999;
274(30):
20943 - 20948.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. K. Magnusson and D. F. Mosher
Fibronectin : Structure, Assembly, and Cardiovascular Implications
Arterioscler Thromb Vasc Biol,
September 1, 1998;
18(9):
1363 - 1370.
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Koyama, M. G. Kinsella, T. N. Wight, U. Hedin, and A. W. Clowes
Heparan Sulfate Proteoglycans Mediate a Potent Inhibitory Signal for Migration of Vascular Smooth Muscle Cells
Circ. Res.,
August 10, 1998;
83(3):
305 - 313.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Corbett, L. Lee, C. L. Wilson, and J. E. Schwarzbauer
Covalent Cross-linking of Fibronectin to Fibrin Is Required for Maximal Cell Adhesion to a Fibronectin-Fibrin Matrix
J. Biol. Chem.,
October 3, 1997;
272(40):
24999 - 25005.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
