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Bone marrow collected 14 days after in vivo administration of granulocyte
colony-stimulating factor and stem cell factor to mice has 10-fold more
repopulating ability than untreated bone marrow
DM Bodine, NE Seidel and D Orlic
Hematopoiesis Section, Laboratory of Gene Transfer/National Center Human
Genome Research, Bethesda, MD 20892-4470, USA.
We have examined the repopulating ability of bone marrow and peripheral
blood cells collected immediately and at intervals after treatment of donor
mice with the combination of granulocyte colony-stimulating factor (G-CSF)
and stem cell factor (SCF). Using a competitive repopulation assay we
showed that the repopulating ability of peripheral blood cells was highest
immediately after cytokine treatment and declined to normal levels within 6
weeks of the termination of treatment with G-CSF and SCF. In contrast the
repopulating ability of bone marrow cells was low immediately after
cytokine treatment and increased to levels that were 10-fold or more
greater than marrow from untreated mice by 14 days after termination of
treatment with G-CSF and SCF. This high level of repopulating activity
declined to normal levels by 6 weeks after termination of treatment with
G-CSF and SCF. The high level of repopulating ability was confirmed by
injecting cells from G- CSF- and SCF-treated donors into unconditioned
recipients. Peripheral blood cells collected immediately after treatment
with G-CSF and SCF engrafted into unconditioned mice sevenfold better than
an equivalent number of bone marrow cells from untreated mice. Likewise,
bone marrow cells collected 14 days after treatment of the donor animal
with G-CSF and SCF engrafted at 10-fold higher levels than an equivalent
number of bone marrow cells from untreated mice. We conclude that the
treatment of donor mice with G-CSF and SCF causes a transient increase in
the repopulating ability of peripheral blood and later of bone marrow.
These observations may have applications to clinical hematopoietic stem
cell transplantation.
Volume 88,
Issue 1,
pp. 89-97,
07/01/1996
Copyright © 1996 by The American Society of Hematology
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