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Primitive hematopoietic cells in murine bone marrow express the CD34
antigen
F Morel, SJ Szilvassy, M Travis, B Chen and A Galy
SyStemix Inc, Palo Alto, CA 94304, USA.
The CD34 antigen is expressed on most, if not all, human hematopoietic stem
cells (HSCs) and hematopoietic progenitor cells, and its use for the
enrichment of HSCs with repopulating potential is well established.
However, despite homology between human and murine CD34, its expression on
subsets of primitive murine hematopoietic cells has not been examined in
full detail. To address this issue, we used a novel monoclonal antibody
against murine CD34 (RAM34) to fractionate bone marrow (BM) cells that were
then assayed in vitro and in vivo with respect to differing functional
properties. A total of 4% to 17% of murine BM cells expressed CD34 at
intermediate to high levels, representing a marked improvement over the
resolution obtained with previously described polyclonal anti-CD34
antibodies. Sixty percent of CD34+ BM cells lacked lineage (Lin) markers
expressed on mature lymphoid or myeloid cells. Eighty-five percent of
Sca-1+Thy-1(10)Lin- /10 cells that are highly enriched in HSCs expressed
intermediate, but not high, levels of CD34 antigen. The remainder of these
phenotypically defined stem cells were CD34-. In vitro colony-forming
cells, day-8 and -12 spleen colony-forming units (CFU-S), primitive
progenitors able to differentiate into B lymphocytes in vitro or into T
lymphocytes in SCID mice, and stem cells with radioprotective and
competitive long-term repopulating activity were all markedly enriched in
the CD34+ fraction after single-parameter cell sorting. In contrast,
CD34-BM cells were depleted of such activities at the cell doses tested and
were capable of only short-term B-cell production in vitro. The results
indicate that a significant proportion of murine HSCs and multilineage
progenitor cells express detectable levels of CD34, and that the RAM34
monoclonal antibody is a useful tool to subset primitive murine
hematopoietic cells. These findings should facilitate more direct
comparisons of the biology of CD34+ murine and human stem and progenitor
cells.
Volume 88,
Issue 10,
pp. 3774-3784,
11/15/1996
Copyright © 1996 by The American Society of Hematology
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