|
|
 Previous Article | Table of Contents | Next Article 
Arachidonic acid mediates interleukin-1 and tumor necrosis factor-alpha-
induced activation of the c-jun amino-terminal kinases in stromal cells
MT Rizzo and C Carlo-Stella
Bone Marrow Transplantation Laboratory, Methodist Cancer Center, Methodist
Hospital, Indianapolis, IN 46202, USA.
We have previously shown that arachidonic acid mediates interleukin-1
(IL-1) and tumor necrosis factor-alpha (TNF-alpha)-induced transcription of
c-jun. The signaling pathway of arachidonic acid- induced c-jun
transcription was independent of protein kinase C activation and involved a
tyrosine kinase-dependent process. The present study was undertaken to
further elucidate the signal transduction pathway of arachidonate-induced
c-jun transcription. We used a glutathione-S-transferase-c-jun fusion
protein containing the aminoterminal domain of c-jun (residues 5 to 89) to
explore the hypothesis that arachidonic acid stimulates c-jun
amino-terminal kinase (JNK) activity in the murine stromal cell line +/+.1
LDA 11. Extracts from arachidonic acid-treated cells catalyzed
phosphorylation of the c- jun fusion protein, indicating stimulation of JNK
activity. Similar results were obtained when cells were challenged with
IL-1 and TNF- alpha. The effect of arachidonic acid was specific, because
extracts from stimulated cells failed to phosphorylate a mutated fusion
protein in which serine 63 and 73 of c-jun were each substituted with
leucine. Arachidonic acid induced JNK activation in a time- and
dose-dependent manner that was not mimicked by saturated fatty acids such
as palmitic acid or other unsaturated fatty acids from the n-3, n-6, or n-9
series. Furthermore, other lipids, such as diacylglycerol, phosphatidic
acid, and C2-ceramide, failed to induce a significant increase in JNK
activity. Treatment of stromal cells with propyl gallate, a dual inhibitor
of lipoxygenase and cyclooxygenase enzymes, did not affect the ability of
arachidonic acid to induce JNK activation. Moreover, ETYA
(5,8,11,14-eicosate-traynoic acid), a nonmetabolizable arachidonate
analogue, also induced JNK activation. These results are consistent with
the hypothesis that the signal transduction pathway by which arachidonate
stimulates c-jun transcription involves activation of the JNK cascade.
Furthermore, arachidonic acid itself and not its cyclooxygenase or
lipoxygenase metabolites is involved in stimulating JNK activity. Thus,
arachidonic acid may act as a second messenger in mediating the effects of
IL-1 and TNF-alpha in the activation of c-jun.
Volume 88,
Issue 10,
pp. 3792-3800,
11/15/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Moussaieff, E. Shohami, Y. Kashman, E. Fride, M. L. Schmitz, F. Renner, B. L. Fiebich, E. Munoz, Y. Ben-Neriah, and R. Mechoulam
Incensole Acetate, a Novel Anti-Inflammatory Compound Isolated from Boswellia Resin, Inhibits Nuclear Factor-{kappa}B Activation
Mol. Pharmacol.,
December 1, 2007;
72(6):
1657 - 1664.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ishikawa, K. Hwang, D. Lazzarino, and P. L. Morris
Sertoli Cell Expression of Steroidogenic Acute Regulatory Protein-Related Lipid Transfer 1 and 5 Domain-Containing Proteins and Sterol Regulatory Element Binding Protein-1 Are Interleukin-1{beta} Regulated by Activation of c-Jun N-Terminal Kinase and Cyclooxygenase-2 and Cytokine Induction
Endocrinology,
December 1, 2005;
146(12):
5100 - 5111.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Burkert, D. Szellas, O. Radmark, D. Steinhilber, and O. Werz
Cell type-dependent activation of 5-lipoxygenase by arachidonic acid
J. Leukoc. Biol.,
January 1, 2003;
73(1):
191 - 200.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kelly, A. Lynch, E. Vereker, Y. Nolan, P. Queenan, E. Whittaker, L. A. J. O'Neill, and M. A. Lynch
The Anti-inflammatory Cytokine, Interleukin (IL)-10, Blocks the Inhibitory Effect of IL-1beta on Long Term Potentiation. A ROLE FOR JNK
J. Biol. Chem.,
November 30, 2001;
276(49):
45564 - 45572.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. GORIN, N.-H. KIM, D. FELIERS, B. BHANDARI, G. G. CHOUDHURY, and H. E. ABBOUD
Angiotensin II activates Akt/protein kinase B by an arachidonic acid/redox-dependent pathway and independent of phosphoinositide 3-kinase
FASEB J,
September 1, 2001;
15(11):
1909 - 1920.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. T. Rizzo, E. Nguyen, M. Aldo-Benson, and G. Lambeau
Secreted phospholipase A2 induces vascular endothelial cell migration
Blood,
December 1, 2000;
96(12):
3809 - 3815.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Vereker, E. O'Donnell, and M. A. Lynch
The Inhibitory Effect of Interleukin-1beta on Long-Term Potentiation Is Coupled with Increased Activity of Stress-Activated Protein Kinases
J. Neurosci.,
September 15, 2000;
20(18):
6811 - 6819.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. T. Rizzo, E. Regazzi, D. Garau, L. Akard, M. Dugan, H. S. Boswell, V. Rizzoli, and C. Carlo-Stella
Induction of Apoptosis by Arachidonic Acid in Chronic Myeloid Leukemia Cells
Cancer Res.,
October 1, 1999;
59(19):
5047 - 5053.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-C. Kim, M.-N. Lee, J.-Y. Kim, S.-S. Lee, J.-D. Chang, S.-S. Kim, S.-Y. Lee, and J.-H. Kim
Roles of Phosphatidylinositol 3-Kinase and Rac in the Nuclear Signaling by Tumor Necrosis Factor-alpha in Rat-2 Fibroblasts
J. Biol. Chem.,
August 20, 1999;
274(34):
24372 - 24377.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Mitchell, C. N. Metz, T. Peng, and R. Bucala
Sustained Mitogen-activated Protein Kinase (MAPK) and Cytoplasmic Phospholipase A2 Activation by Macrophage Migration Inhibitory Factor (MIF). REGULATORY ROLE IN CELL PROLIFERATION AND GLUCOCORTICOID ACTION
J. Biol. Chem.,
June 18, 1999;
274(25):
18100 - 18106.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. S. T. Hii, Z. H. Huang, A. Bilney, M. Costabile, A. W. Murray, D. A. Rathjen, C. J Der, and A. Ferrante
Stimulation of p38 Phosphorylation and Activity by Arachidonic Acid in HeLa Cells, HL60 Promyelocytic Leukemic Cells, and Human Neutrophils. EVIDENCE FOR CELL TYPE-SPECIFIC ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES
J. Biol. Chem.,
July 24, 1998;
273(30):
19277 - 19282.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. K. Tam, S. Schotland, and J. Green
Inflammatory cytokines (IL-1alpha , TNF-alpha ) and LPS modulate the Ca2+ signaling pathway in osteoblasts
Am J Physiol Cell Physiol,
June 1, 1998;
274(6):
C1686 - C1698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C. LaPointe and J. R. Sitkins
Phospholipase A2 Metabolites Regulate Inducible Nitric Oxide Synthase in Myocytes
Hypertension,
January 1, 1998;
31(1):
218 - 224.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Thibonnier, J. A. Preston, N. Dulin, P. L. Wilkins, L. N. Berti-Mattera, and R. Mattera
The Human V3 Pituitary Vasopressin Receptor: Ligand Binding Profile and Density-Dependent Signaling Pathways
Endocrinology,
October 1, 1997;
138(10):
4109 - 4122.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. S. T. Hii, N. Moghadammi, A. Dunbar, and A. Ferrante
Activation of the Phosphatidylinositol 3-Kinase-Akt/Protein Kinase B Signaling Pathway in Arachidonic Acid-stimulated Human Myeloid and Endothelial Cells. INVOLVEMENT OF THE ErbB RECEPTOR FAMILY
J. Biol. Chem.,
July 13, 2001;
276(29):
27246 - 27255.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
