|
|
 Previous Article | Table of Contents | Next Article 
Human fibroblasts downregulate plasminogen activator inhibitor type-1 in
cultured human macrovascular and microvascular endothelial cells
JC Zhang, A Fabry, L Paucz, J Wojta and BR Binder
Department of Vascular Biology and Thrombosis Research, University of
Vienna, Austria.
We have previously reported that plasminogen activator inhibitor type-1
(PAI-1) expression in endothelial cells (ECs) can be modulated differently
by smooth muscle cells depending on their origin. Human pulmonary artery
smooth muscle cells (HPASMCs) strongly downregulated PAI-1 expression in
ECs. Fibroblasts (FBs) are another cell type that could come in close
contact with ECs. Therefore, it was the aim of this study to investigate
whether FBs could also influence the fibrinolytic potential of ECs. As in
the case of HPASMCs, PAI-1 antigen produced by human umbilical vein ECs
(HUVECs) cocultured with human skin FBs (HSFBs) was significantly lower as
compared with the sum of PAI-1 secreted by the respective cell types
cultured separately. Not only HUVECs but also human skin microvascular ECs
(HSMECs) responded in a dose-dependent way to serum-free conditioned media
(CM) from HSFBs from one individual donor. Similar results were obtained
when CM from HSFBs from four other individual donors were used. PAI-1 mRNA
decreased in HUVECs incubated for 6 hours with HSFB-CM to 24% to 55% of
control, depending on the preparation of HSFBs used. A significant PAI-1
downregulatory effect was only observed when CM from low-passage HSFBs (up
to passage no. 5) was used, whereas no reduction in EC PAI-1 production was
observed with CM obtained from HSFBs in passage no. 8. This PAI-1
downregulatory activity present in HSFB-CM was heat-labile and had a
molecular mass of approximately 5 kD. When CM from HPASMCs was analyzed in
the same way, an almost identical elution profile was found. In conclusion,
our data showed that FBs can decrease the expression of PAI-1 in ECs. Such
an effect could be operative during wound-healing and at other capillary
sites where FBs could render ECs profibrinolytic, thereby facilitating
processes requiring an increase in proteolytic activity such as EC
migration and proliferation.
Volume 88,
Issue 10,
pp. 3880-3886,
11/15/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Huber, A. Furnkranz, V. N. Bochkov, M. K. Patricia, H. Lee, C. C. Hedrick, J. A. Berliner, B. R. Binder, and N. Leitinger
Specific monocyte adhesion to endothelial cells induced by oxidized phospholipids involves activation of cPLA2 and lipoxygenase
J. Lipid Res.,
May 1, 2006;
47(5):
1054 - 1062.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Kroismayr, U. Baranyi, C. Stehlik, A. Dorfleutner, B. R. Binder, and J. Lipp
HERC5, a HECT E3 ubiquitin ligase tightly regulated in LPS activated endothelial cells
J. Cell Sci.,
September 15, 2004;
117(20):
4749 - 4756.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Mayer, M. Bilban, V. Kurtev, F. Gruber, O. Wagner, B. R. Binder, and R. de Martin
Deciphering Regulatory Patterns of Inflammatory Gene Expression From Interleukin-1--Stimulated Human Endothelial Cells
Arterioscler Thromb Vasc Biol,
July 1, 2004;
24(7):
1192 - 1198.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Maekawa, Y. Oike, S. Kanda, Y. Ito, Y. Yamada, H. Kurihara, R. Nagai, and T. Suda
Ephrin-B2 Induces Migration of Endothelial Cells Through the Phosphatidylinositol-3 Kinase Pathway and Promotes Angiogenesis in Adult Vasculature
Arterioscler Thromb Vasc Biol,
November 1, 2003;
23(11):
2008 - 2014.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ebner, A. Bandion, B. R. Binder, R. de Martin, and J. A. Schmid
GMCSF activates NF-{kappa}B via direct interaction of the GMCSF receptor with I{kappa}B kinase {beta}
Blood,
July 1, 2003;
102(1):
192 - 199.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Huber, H. Boechzelt, B. Karten, M. Surboeck, V. N. Bochkov, B. R. Binder, W. Sattler, and N. Leitinger
Oxidized Cholesteryl Linoleates Stimulate Endothelial Cells to Bind Monocytes via the Extracellular Signal-Regulated Kinase 1/2 Pathway
Arterioscler Thromb Vasc Biol,
April 1, 2002;
22(4):
581 - 586.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Huber, A. Vales, G. Mitulovic, M. Blumer, R. Schmid, J. L. Witztum, B. R. Binder, and N. Leitinger
Oxidized Membrane Vesicles and Blebs From Apoptotic Cells Contain Biologically Active Oxidized Phospholipids That Induce Monocyte-Endothelial Interactions
Arterioscler Thromb Vasc Biol,
January 1, 2002;
22(1):
101 - 107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Oitzinger, R. Hofer-Warbinek, J. A. Schmid, Y. Koshelnick, B. R. Binder, and R. de Martin
Adenovirus-mediated expression of a mutant I{kappa}B kinase 2 inhibits the response of endothelial cells to inflammatory stimuli
Blood,
March 15, 2001;
97(6):
1611 - 1617.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-C. ZHANG, K. CLAFFEY, R. SAKTHIVEL, Z. DARZYNKIEWICZ, D. E. SHAW, J. LEAL, Y.-C. WANG, F.-M. LU, and K. R. MCCRAE
Two-chain high molecular weight kininogen induces endothelial cell apoptosis and inhibits angiogenesis: partial activity within domain 5
FASEB J,
December 1, 2000;
14(15):
2589 - 2600.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Holzmuller, T. Moll, R. Hofer-Warbinek, D. Mechtcheriakova, B. R. Binder, and E. Hofer
A Transcriptional Repressor of the Tissue Factor Gene in Endothelial Cells
Arterioscler Thromb Vasc Biol,
July 1, 1999;
19(7):
1804 - 1811.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Mechtcheriakova, A. Wlachos, H. Holzmuller, B. R. Binder, and E. Hofer
Vascular Endothelial Cell Growth Factor-Induced Tissue Factor Expression in Endothelial Cells Is Mediated by EGR-1
Blood,
June 1, 1999;
93(11):
3811 - 3823.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-C. Zhang, R. Sakthivel, D. Kniss, C. H. Graham, D. K. Strickland, and K. R. McCrae
The Low Density Lipoprotein Receptor-related Protein/alpha 2-Macroglobulin Receptor Regulates Cell Surface Plasminogen Activator Activity on Human Trophoblast Cells
J. Biol. Chem.,
November 27, 1998;
273(48):
32273 - 32280.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Isumi, N. Minamino, T. Katafuchi, M. Yoshioka, T. Tsuji, K. Kangawa, and H. Matsuo
Adrenomedullin Production in Fibroblasts: Its Possible Function as a Growth Regulator of Swiss 3T3 Cells
Endocrinology,
May 1, 1998;
139(5):
2552 - 2563.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Hofer-Warbinek, J. A. Schmid, C. Stehlik, B. R. Binder, J. Lipp, and R. de Martin
Activation of NF-kappa B by XIAP, the X Chromosome-linked Inhibitor of Apoptosis, in Endothelial Cells Involves TAK1
J. Biol. Chem.,
July 14, 2000;
275(29):
22064 - 22068.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Huber, H. Boechzelt, B. Karten, M. Surboeck, V. N. Bochkov, B. R. Binder, W. Sattler, and N. Leitinger
Oxidized Cholesteryl Linoleates Stimulate Endothelial Cells to Bind Monocytes via the Extracellular Signal-Regulated Kinase 1/2 Pathway
Arterioscler Thromb Vasc Biol,
April 1, 2002;
22(4):
581 - 586.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
