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The cDNA and derived amino acid sequence of porcine factor VIII
JF Healey, IM Lubin and P Lollar
Department of Medicine, Emory University, Atlanta GA 30322, USA.
The cDNA corresponding to 137 bp of the 5' untranslated region, the signal
peptide, and the A1, A3, C1, and C2 domains of porcine factor VIII (fVIII)
have been cloned and sequenced. Along with previously determined sequences
of the porcine fVIII B domain and the A2 domain, this completes the
sequence determination of the cDNA corresponding to the translated protein.
Alignments of the derived amino acid sequence of porcine fVIII with human
and murine fVIII indicate that the A1, A2, A3, C1, and C2 domains are more
conserved than the B domains or the proteolytic cleavage peptides
corresponding to residues 337-372 and 1649-1689. The knowledge of the
porcine fVIII cDNA may be useful to understand functional and immunological
differences between human and porcine fVIII and may lead to improved fVIII
replacement products for hemophilia. A patients through the development of
recombinant porcine fVIII or hybrid human/porcine fVIII derivatives.
Volume 88,
Issue 11,
pp. 4209-4214,
12/01/1996
Copyright © 1996 by The American Society of Hematology
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