|
|
 Previous Article | Table of Contents | Next Article 
Schistosoma mansoni excretory circulating cathodic antigen shares Lewis- x
epitopes with a human granulocyte surface antigen and evokes host
antibodies mediating complement-dependent lysis of granulocytes
GJ van Dam, FH Claas, M Yazdanbakhsh, YC Kruize, AC van Keulen, ST Ferreira, JP Rotmans and AM Deelder
Department of Parasitology, University of Leiden, The Netherlands.
Parasitic worms of the genus Schistosoma excrete relatively large amounts
of immunogenic glycoproteins (circulating cathodic antigen [CCA]) that
contain polysaccharide side chains with the trisaccharide Lewis-x (L(ex))
as a repeating unit. These carbohydrates evoke high titers of specific IgM
antibodies that cross-react with the repeating L(ex) units on the surface
of granulocytes. Consequently this might lead, in the presence of
complement, to lysis of the granulocytes. In the present study, this
hypothesis was investigated using anti-CCA mouse monoclonal antibodies
(MoAbs) and polyclonal antibodies purified from sera of infected humans. By
flow cytometry, it was demonstrated that the mouse MoAbs directed against
CCA strongly recognized the granulocytes. It could also be shown that these
MoAbs, as well as anti- CCA IgM antibodies purified from infected human
sera, caused lysis of granulocytes in a complement-dependent cytotoxicity
assay. Sera from healthy controls or from patients with other helminth
infections resulted in negligible granulocytotoxicity. These in vitro
observed phenomena may explain the mild to moderate neutropenia that occurs
in schistosomiasis patients.
Volume 88,
Issue 11,
pp. 4246-4251,
12/01/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Jang-Lee, R. S. Curwen, P. D. Ashton, B. Tissot, W. Mathieson, M. Panico, A. Dell, R. A. Wilson, and S. M. Haslam
Glycomics Analysis of Schistosoma mansoni Egg and Cercarial Secretions
Mol. Cell. Proteomics,
September 1, 2007;
6(9):
1485 - 1499.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Ma, J. L. Simala-Grant, and D. E. Taylor
Fucosylation in prokaryotes and eukaryotes
Glycobiology,
December 1, 2006;
16(12):
158R - 184R.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. van Die, S. J. van Vliet, A. K. Nyame, R. D. Cummings, C. M.C. Bank, B. Appelmelk, T. B.H. Geijtenbeek, and Y. van Kooyk
The dendritic cell-specific C-type lectin DC-SIGN is a receptor for Schistosoma mansoni egg antigens and recognizes the glycan antigen Lewis x
Glycobiology,
June 1, 2003;
13(6):
471 - 478.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. van Remoortere, G. J. van Dam, C. H. Hokke, D. H. van den Eijnden, I. van Die, and A. M. Deelder
Profiles of Immunoglobulin M (IgM) and IgG Antibodies against Defined Carbohydrate Epitopes in Sera of Schistosoma-Infected Individuals Determined by Surface Plasmon Resonance
Infect. Immun.,
April 1, 2001;
69(4):
2396 - 2401.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. v. Remoortere, C. H.Hokke, G. J.v. Dam, I. v. Die, A. M.Deelder, and D. H.v. d. Eijnden
Various stages of Schistosoma express Lewisx, LacdiNAc, GalNAc{beta}1-4 (Fuc{alpha}1-3)GlcNAc and GalNAc{beta}1-4(Fuc{alpha}1-2Fuc{alpha}1-3)GlcNAc carbohydrate epitopes: detection with monoclonal antibodies that are characterized by enzymatically synthesized neoglycoproteins
Glycobiology,
June 1, 2000;
10(6):
601 - 609.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
