|
|
 Previous Article | Table of Contents | Next Article 
Thrombopoietin supports proliferation of human primitive hematopoietic
cells in synergy with steel factor and/or interleukin-3
M Kobayashi, JH Laver, T Kato, H Miyazaki and M Ogawa
Department of Pediatrics and Medicine, Medical University of South
Carolina, Charleston, USA.
We have studied the effects of recombinant human thrombopoietin (TPO; mpl
ligand) on the proliferation of human primitive hematopoietic progenitors
in vitro. CD34+ cells were enriched for cell-cycle-dormant primitive
progenitors by separation on the basis of expression of c-kit and CD38. In
the presence of varying combinations of TPO, Steel factor (SF), and
interleukin-3 (IL-3), CD34+/c-kit(low)/CD38neg/low cells produced fewer
colonies than CD34+/c-kit(low)/CD38high cells. However, when cultured in
suspension for 7 days and replated in methylcellulose culture for
measurement of colony-forming cells, the former population generated more
colony-forming cells than the latter. In suspension culture of
CD34+/c-kit(low)/CD38neg/low cells, TPO acted synergistically with SF
and/or IL-3 in support of the production of colony-forming cells for
granulocyte/macrophage colonies, erythroid colonies, and mixed colonies.
Culture studies of individual CD34+/c- kit(low)/CD38neg/low cells provided
the evidence for the direct nature of the effects of TPO. When combined
with SF, TPO showed stronger stimulation of production of progenitors in
suspension culture than other early-acting factors, such as IL-6, IL-11,
and granulocyte colony- stimulating factor (G-CSF). TPO may be an important
cytokine for in vitro manipulation of human hematopoietic stem cells.
Volume 88,
Issue 2,
pp. 429-436,
07/15/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K. Saito, M. Hirokawa, K. Inaba, H. Fukaya, Y. Kawabata, A. Komatsuda, J. Yamashita, and K. Sawada
Phagocytosis of codeveloping megakaryocytic progenitors by dendritic cells in culture with thrombopoietin and tumor necrosis factor-{alpha} and its possible role in hemophagocytic syndrome
Blood,
February 15, 2006;
107(4):
1366 - 1374.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kawaguchi, M. Kobayashi, K. Nakamura, N. Konishi, S.-i. Miyagawa, T. Sato, H. Toyoda, Y. Komada, S. Kojima, Y. Todoroki, et al.
Dysregulation of transcriptions in primary granule constituents during myeloid proliferation and differentiation in patients with severe congenital neutropenia
J. Leukoc. Biol.,
February 1, 2003;
73(2):
225 - 234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. M. Linden and K. Kaushansky
The Glycan Domain of Thrombopoietin (TPO) Acts in trans to Enhance Secretion of the Hormone and Other Cytokines
J. Biol. Chem.,
September 13, 2002;
277(38):
35240 - 35247.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ballmaier, M. Germeshausen, H. Schulze, K. Cherkaoui, S. Lang, A. Gaudig, S. Krukemeier, M. Eilers, G. Strau{beta}, and K. Welte
c-mpl mutations are the cause of congenital amegakaryocytic thrombocytopenia
Blood,
January 1, 2001;
97(1):
139 - 146.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Nakamura, M. Kobayashi, N. Konishi, H. Kawaguchi, S.-i. Miyagawa, T. Sato, H. Toyoda, Y. Komada, S. Kojima, O. Katoh, et al.
Abnormalities of primitive myeloid progenitor cells expressing granulocyte colony-stimulating factor receptor in patients with severe congenital neutropenia
Blood,
December 15, 2000;
96(13):
4366 - 4369.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Isomura, M. Yoshida, H. Namba, N. Fujiwara, R. Ohuchi, F. Uno, M. Oda, Y. Seino, and M. Yamada
Suppressive effects of human herpesvirus-6 on thrombopoietin-inducible megakaryocytic colony formation in vitro
J. Gen. Virol.,
March 1, 2000;
81(3):
663 - 673.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. Kuramoto, T. Uesaka, A. Kimura, M. Kobayashi, H. Watanabe, and O. Katoh
ZK7, a Novel Zinc Finger Gene, Is Induced by Vascular Endothelial Growth Factor and Inhibits Apoptotic Death in Hematopoietic Cells
Cancer Res.,
January 1, 2000;
60(2):
425 - 430.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. Konishi, M. Kobayashi, S.-i. Miyagawa, T. Sato, O. Katoh, and K. Ueda
Defective Proliferation of Primitive Myeloid Progenitor Cells in Patients With Severe Congenital Neutropenia
Blood,
December 15, 1999;
94(12):
4077 - 4083.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. S. Kirshenbaum, J. P. Goff, T. Semere, B. Foster, L. M. Scott, and D. D. Metcalfe
Demonstration That Human Mast Cells Arise From a Progenitor Cell Population That Is CD34+, c-kit+, and Expresses Aminopeptidase N (CD13)
Blood,
October 1, 1999;
94(7):
2333 - 2342.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-C. Shih, M. C.-T. Hu, J. Hu, J. Medeiros, and S. J. Forman
Long-Term Ex Vivo Maintenance and Expansion of Transplantable Human Hematopoietic Stem Cells
Blood,
September 1, 1999;
94(5):
1623 - 1636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Sawai, K. Koike, H. H. Mwamtemi, T. Kinoshita, Y. Kurokawa, K. Sakashita, T. Higuchi, K. Takeuchi, M. Shiohara, T. Kamijo, et al.
Thrombopoietin Augments Stem Cell Factor-Dependent Growth of Human Mast Cells From Bone Marrow Multipotential Hematopoietic Progenitors
Blood,
June 1, 1999;
93(11):
3703 - 3712.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Sawai, K. Koike, S. Ito, H. H. Mwamtemi, Y. Kurokawa, T. Kinoshita, K. Sakashita, T. Higuchi, K. Takeuchi, M. Shiohara, et al.
Neutrophilic Cell Production by Combination of Stem Cell Factor and Thrombopoietin From CD34+ Cord Blood Cells in Long-Term Serum-Deprived Liquid Culture
Blood,
January 15, 1999;
93(2):
509 - 518.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Goff, D. S. Shields, and J. S. Greenberger
Influence of Cytokines on the Growth Kinetics and Immunophenotype of Daughter Cells Resulting From the First Division of Single CD34+Thy-1+lin- Cells
Blood,
December 1, 1998;
92(11):
4098 - 4107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Gothot, J. C.M. van der Loo, D. W. Clapp, and E. F. Srour
Cell Cycle-Related Changes in Repopulating Capacity of Human Mobilized Peripheral Blood CD34+ Cells in Non-Obese Diabetic/Severe Combined Immune-Deficient Mice
Blood,
October 15, 1998;
92(8):
2641 - 2649.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kaplan, S. Kaplan, K. F. Marcoe, L. R. Sauvage, and W. P. Hammond
The Effect of Hematopoietic Growth Factors on Platelet Aggregability
Clinical and Applied Thrombosis/Hemostasis,
October 1, 1998;
4(4):
238 - 242.
[Abstract]
[PDF]
|
|
|
|
|
|
|
K. J. Neelis, T. P. Visser, W. Dimjati, G. R. Thomas, P. J. Fielder, D. Bloedow, D. L. Eaton, and G. Wagemaker
A Single Dose of Thrombopoietin Shortly After Myelosuppressive Total Body Irradiation Prevents Pancytopenia in Mice by Promoting Short-Term Multilineage Spleen-Repopulating Cells at the Transient Expense of Bone Marrow-Repopulating Cells
Blood,
September 1, 1998;
92(5):
1586 - 1597.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Matsunaga, F. Hirayama, Y. Yonemura, R. Murray, and M. Ogawa
Negative Regulation by Interleukin-3 (IL-3) of Mouse Early B-Cell Progenitors and Stem Cells in Culture: Transduction of the Negative Signals by beta c and beta IL-3 Proteins of IL-3 Receptor and Absence of Negative Regulation by Granulocyte-Macrophage Colony-Stimulating Factor
Blood,
August 1, 1998;
92(3):
901 - 907.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Matsunaga, T. Kato, H. Miyazaki, and M. Ogawa
Thrombopoietin Promotes the Survival of Murine Hematopoietic Long-Term Reconstituting Cells: Comparison With the Effects of FLT3/FLK-2 Ligand and Interleukin-6
Blood,
July 15, 1998;
92(2):
452 - 461.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. P. Solar, W. G. Kerr, F. C. Zeigler, D. Hess, C. Donahue, F. J. de Sauvage, and D. L. Eaton
Role of c-mpl in Early Hematopoiesis
Blood,
July 1, 1998;
92(1):
4 - 10.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Sawai, K. Koike, T. Higuchi, K. Ogami, and M. Oda
Thrombopoietin Enhances the Production of Myeloid Cells, but not Megakaryocytes, in Juvenile Chronic Myelogenous Leukemia
Blood,
June 1, 1998;
91(11):
4065 - 4073.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Williams, G. G. Pipia, N. S. Datta, and M. W. Long
Thrombopoietin Requires Additional Megakaryocyte-Active Cytokines for Optimal Ex Vivo Expansion of Megakaryocyte Precursor Cells
Blood,
June 1, 1998;
91(11):
4118 - 4126.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Orlic, L. J. Girard, S. M. Anderson, L. C. Pyle, M. C. Yoder, H. E. Broxmeyer, and D. M. Bodine
Identification of Human and Mouse Hematopoietic Stem Cell Populations Expressing High Levels of mRNA Encoding Retrovirus Receptors
Blood,
May 1, 1998;
91(9):
3247 - 3254.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. D. Lyman and S. E. W. Jacobsen
c-kit Ligand and Flt3 Ligand: Stem/Progenitor Cell Factors With Overlapping Yet Distinct Activities
Blood,
February 15, 1998;
91(4):
1101 - 1134.
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. M. Luens, M. A. Travis, B. P. Chen, B. L. Hill, R. Scollay, and L. J. Murray
Thrombopoietin, kit Ligand, and flk2/flt3 Ligand Together Induce Increased Numbers of Primitive Hematopoietic Progenitors From Human CD34+Thy-1+Lin- Cells With Preserved Ability to Engraft SCID-hu Bone
Blood,
February 15, 1998;
91(4):
1206 - 1215.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Dao, A. J. Shah, G. M. Crooks, and J. A. Nolta
Engraftment and Retroviral Marking of CD34+ and CD34+CD38- Human Hematopoietic Progenitors Assessed in Immune-Deficient Mice
Blood,
February 15, 1998;
91(4):
1243 - 1255.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kimura, A. W. Roberts, D. Metcalf, and W. S. Alexander
Hematopoietic stem cell deficiencies in mice lacking c-Mpl, the receptor for thrombopoietin
PNAS,
February 3, 1998;
95(3):
1195 - 1200.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. M. Frey, S. Rafii, M. Teterson, D. Eaton, R. G. Crystal, and M. A.S. Moore
Adenovector-Mediated Expression of Human Thrombopoietin cDNA in Immune-Compromised Mice: Insights into the Pathophysiology of Osteomyelofibrosis
J. Immunol.,
January 15, 1998;
160(2):
691 - 699.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Shibuya, H. Akahori, K. Takahashi, E. Tahara, T. Kato, and H. Miyazaki
Multilineage Hematopoietic Recovery by a Single Injection of Pegylated Recombinant Human Megakaryocyte Growth and Development Factor in Myelosuppressed Mice
Blood,
January 1, 1998;
91(1):
37 - 45.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. H. Yin, S. Miraglia, E. D. Zanjani, G. Almeida-Porada, M. Ogawa, A. G. Leary, J. Olweus, J. Kearney, and D. W. Buck
AC133, a Novel Marker for Human Hematopoietic Stem and Progenitor Cells
Blood,
December 15, 1997;
90(12):
5002 - 5012.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Gothot, R. Pyatt, J. McMahel, S. Rice, and E. F. Srour
Functional Heterogeneity of Human CD34+ Cells Isolated in Subcompartments of the G0 /G1 Phase of the Cell Cycle
Blood,
December 1, 1997;
90(11):
4384 - 4393.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Guerriero, L. Worford, H. K. Holland, G.-R. Guo, K. Sheehan, and E. K. Waller
Thrombopoietin Is Synthesized by Bone Marrow Stromal Cells
Blood,
November 1, 1997;
90(9):
3444 - 3455.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. J. Borge, V. Ramsfjell, L. Cui, and S. E.W. Jacobsen
Ability of Early Acting Cytokines to Directly Promote Survival and Suppress Apoptosis of Human Primitive CD34+CD38- Bone Marrow Cells With Multilineage Potential at the Single-Cell Level: Key Role of Thrombopoietin
Blood,
September 15, 1997;
90(6):
2282 - 2292.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bhatia, D. Bonnet, U. Kapp, J. C.Y. Wang, B. Murdoch, and J. E. Dick
Quantitative Analysis Reveals Expansion of Human Hematopoietic Repopulating Cells After Short-term Ex Vivo Culture
J. Exp. Med.,
August 18, 1997;
186(4):
619 - 624.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. C. Broudy
Stem Cell Factor and Hematopoiesis
Blood,
August 15, 1997;
90(4):
1345 - 1364.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. Ladd, R. Pyatt, A. Gothot, S. Rice, J. McMahel, C. M. Traycoff, and E. F. Srour
Orderly Process of Sequential Cytokine Stimulation Is Required for Activation and Maximal Proliferation of Primitive Human Bone Marrow CD34+ Hematopoietic Progenitor Cells Residing in G0
Blood,
July 15, 1997;
90(2):
658 - 668.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Piacibello, F. Sanavio, L. Garetto, A. Severino, D. Bergandi, J. Ferrario, F. Fagioli, M. Berger, and M. Aglietta
Extensive Amplification and Self-Renewal of Human Primitive Hematopoietic Stem Cells From Cord Blood
Blood,
April 15, 1997;
89(8):
2644 - 2653.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. C. Broudy, N. L. Lin, D. F. Sabath, T. Papayannopoulou, and K. Kaushansky
Human Platelets Display High-Affinity Receptors for Thrombopoietin
Blood,
March 15, 1997;
89(6):
1896 - 1904.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
