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Characterization of peripheral blood stem cells in mice
Y Yamamoto, R Yasumizu, Y Amou, N Watanabe, N Nishio, J Toki, S Fukuhara and S Ikehara
1st Department of Pathology, Kansai Medical University, Osaka, Japan.
Peripheral blood stem cells (PBSCs) were mobilized in mice by treatment
with cytosine-arabinoside on day 0, followed by the administration by
injection of granulocyte colony-stimulating factor for 4 days. There were
remarkable increases in the numbers of cells with lineage-negative (Lin-)
c-kit+ markers, cells with colony-forming unit-cell (CFU-C) and
colony-forming unit-spleen (CFU-S) activities, and cells with marrow-
repopulating ability (MRA) in the extramedullary sites (the spleen,
peripheral blood, and liver) on day 5, whereas the number of these immature
hematopoietic cells decreased in the bone marrow (BM) on day 5. This
finding suggests the mobilization of immature hematopoietic cells from the
BM to the extramedullary sites. Three-color flow cytometric analyses showed
that CD4 antigen was not expressed on the Lin-Sca-1+ cells in the mobilized
PB cells (PBCs), although CD4lo cells were found in those of normal BM
cells. Lin-c-kit+ cells in the mobilized PBCs contained more cells with
immature phenotypes (Sca-1+, Thy1.2lo, CD71-, and Rh123dull) than in normal
BMCs, indicating an alteration of the hierarchical composition of the
Lin-c-kit+ cells. The Lin-c-kit+Sca-1+ cells in the mobilized PBCs had
similar CFU-C and CFU- S activities to those in normal BMCs. Electron
microscopic studies of these cells in the mobilized PBCs showed that only
10% to 20% of these cells had a thin rim of cytoplasm with poorly developed
organelles. Allogeneic transplantation [B6 --> C3H] of PBSCs showed
long-term reconstituting activity across the major histocompatibility
complex barrier 24 weeks after transplantation, although longer observation
is necessary.
Volume 88,
Issue 2,
pp. 445-454,
07/15/1996
Copyright © 1996 by The American Society of Hematology
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