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The supportive effects of erythropoietin and mast cell growth factor on
CD34+/CD36- sorted bone marrow cells of myelodysplasia patients
S Brada, J de Wolf, D Hendriks, M Esselink, M Ruiters and E Vellenga
Department of Hematology, University Groningen, The Netherlands.
In the present study, we analyzed the capacity of CD34+/CD36- sorted bone
marrow cells of myelodysplasia patients (n = 4) to differentiate along the
erythroid lineage in the presence of erythropoietin (Epo) and mast cell
growth factor (MGF). Two subgroups could be identified. In 6 patients, a
normal number of burst-forming units-erythroid (BFU-Es) were cultured from
CD34+/CD36- sorted cells. Cells from these patients did have the capacity
to differentiate to colony-forming units- erythroid (CFU-Es) progenitors in
cell suspension cultures with Epo plus MGF followed by Epo in the culture
assay. Moreover, the cells became CD34-/CD36+/gly-cophorin A (GpA)+ after 7
days of culture with Epo plus MGF, a pattern comparable to that of normal
progenitors. In contrast, in 8 patients, a different pattern was observed.
No BFU-Es or a low number of BFU-Es were cultured from the CD34+/CD36-
sorted cell fraction that was, in most of the cases, incapable of
differentiating to CFU-E progenitors. Flow cytometry of the sorted
population showed that, after 7 days of culture with Epo plus MGF, a high
proportion of CD34+/CD36- cells persisted, whereas a low proportion of
cells became CD34-/CD36+/GpA+. The unresponsiveness is not caused by the
used growth factor combination, because the addition of interleukin-3 did
not correct the defect. Evi-1 expression was studied in 9 cases to show
whether an aberrant Evi-1 expression correlates with a disturbed erythroid
development. Evi-1 expression was shown in 4 of 9 cases, whereas 3 of 9
cases did have a disturbed erythroid differentiation. In summary, the
results show that the defects in the erythroid development in a
subpopulation of patients with myelodysplasia is localized at an early
stage of the erythroid differentiation and is associated with the
persistent expression of the CD34 antigen and, in some cases, with the
expression of Evi-1.
Volume 88,
Issue 2,
pp. 505-510,
07/15/1996
Copyright © 1996 by The American Society of Hematology
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