|
|
 Previous Article | Table of Contents | Next Article 
Direct infection of CD34+ progenitor cells by human cytomegalovirus:
evidence for inhibition of hematopoiesis and viral replication
M Movassagh, J Gozlan, B Senechal, C Baillou, JC Petit and FM Lemoine
Laboratory for Biology and Therapy of Immune Pathology, Hospital Pitie
Salpetriere, Paris, France.
We successfully infected fluorescence-activated cell-sorted CD34+ cells
from normal cord blood by the human cytomegalovirus (HCMV) laboratory
strain Towne. An inhibitory effect of HCMV on clonogenic myeloid
progenitors was observed in primary methylcellulose cultures. After an
initial 7-day liquid culture of CD34(+)-infected cells, this inhibition was
further amplified in secondary methylcellulose cultures, then involving
both the myeloid and erythroid lineages. Under these conditions, viral DNA
was detected both in erythroid and myeloid colonies using the polymerase
chain reaction (PCR), but reverse transcription PCR (RT-PCR) failed to
detect viral RNA. In contrast, when CD34(+)-infected cells were maintained
in liquid suspension, both immediate, early, and late transcripts were
detected as soon as day 3. In addition, viral production was demonstrated
in the culture supernatants, thus confirming that a complete viral cycle
occurred under liquid conditions. Furthermore, by resorting cells into
CD34+ and CD34- fractions, we showed by RT-PCR that viral replication took
place in cells still expressing CD34 antigen, whereas no RNA was found in
more differentiated cells that had subsequently lost their CD34 antigen.
These findings suggest that HCMV replication can occur at the early steps
of progenitor differentiation and may be involved in the viral-induced
myelosuppression.
Volume 88,
Issue 4,
pp. 1277-1283,
08/15/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
F. Goodrum, M. Reeves, J. Sinclair, K. High, and T. Shenk
Human cytomegalovirus sequences expressed in latently infected individuals promote a latent infection in vitro
Blood,
August 1, 2007;
110(3):
937 - 945.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Wu, J. Vieira, N. Fiore, P. Banerjee, M. Sieburg, R. Rochford, W. Harrington Jr, and G. Feuer
KSHV/HHV-8 infection of human hematopoietic progenitor (CD34+) cells: persistence of infection during hematopoiesis in vitro and in vivo
Blood,
July 1, 2006;
108(1):
141 - 151.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. B. Reeves, P. J. Lehner, J. G. P. Sissons, and J. H. Sinclair
An in vitro model for the regulation of human cytomegalovirus latency and reactivation in dendritic cells by chromatin remodelling
J. Gen. Virol.,
November 1, 2005;
86(11):
2949 - 2954.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. B. Reeves, P. A. MacAry, P. J. Lehner, J. G. P. Sissons, and J. H. Sinclair
Latency, chromatin remodeling, and reactivation of human cytomegalovirus in the dendritic cells of healthy carriers
PNAS,
March 15, 2005;
102(11):
4140 - 4145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Andre-Garnier, N. Milpied, D. Boutolleau, S. Saiagh, S. Billaudel, and B.-M. Imbert-Marcille
Reactivation of human herpesvirus 6 during ex vivo expansion of circulating CD34+ haematopoietic stem cells
J. Gen. Virol.,
November 1, 2004;
85(11):
3333 - 3336.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Gredmark, W. B. Britt, X. Xie, L. Lindbom, and C. Soderberg-Naucler
Human Cytomegalovirus Induces Inhibition of Macrophage Differentiation by Binding to Human Aminopeptidase N/CD13
J. Immunol.,
October 15, 2004;
173(8):
4897 - 4907.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Gredmark, T. Tilburgs, and C. Soderberg-Naucler
Human Cytomegalovirus Inhibits Cytokine-Induced Macrophage Differentiation
J. Virol.,
October 1, 2004;
78(19):
10378 - 10389.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Goodrum, C. T. Jordan, S. S. Terhune, K. High, and T. Shenk
Differential outcomes of human cytomegalovirus infection in primitive hematopoietic cell subpopulations
Blood,
August 1, 2004;
104(3):
687 - 695.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. D. Goodrum, C. T. Jordan, K. High, and T. Shenk
Human cytomegalovirus gene expression during infection of primary hematopoietic progenitor cells: A model for latency
PNAS,
December 10, 2002;
99(25):
16255 - 16260.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Forman, R. M. Welsh, T. G. Markees, B. A. Woda, J. P. Mordes, A. A. Rossini, and D. L. Greiner
Viral Abrogation of Stem Cell Transplantation Tolerance Causes Graft Rejection and Host Death by Different Mechanisms
J. Immunol.,
June 15, 2002;
168(12):
6047 - 6056.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Mirandola, P. Secchiero, S. Pierpaoli, G. Visani, L. Zamai, M. Vitale, S. Capitani, and G. Zauli
Infection of CD34+ hematopoietic progenitor cells by human herpesvirus 7 (HHV-7)
Blood,
July 1, 2000;
96(1):
126 - 131.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Isomura, M. Yoshida, H. Namba, N. Fujiwara, R. Ohuchi, F. Uno, M. Oda, Y. Seino, and M. Yamada
Suppressive effects of human herpesvirus-6 on thrombopoietin-inducible megakaryocytic colony formation in vitro
J. Gen. Virol.,
March 1, 2000;
81(3):
663 - 673.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. Crapnell, E. D. Zanjani, A. Chaudhuri, J. L. Ascensao, S. St. Jeor, and J. P. Maciejewski
In vitro infection of megakaryocytes and their precursors by human cytomegalovirus
Blood,
January 15, 2000;
95(2):
487 - 493.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Iwata, J. Vieira, M. Byrne, H. Horton, and B. Torok-Storb
Interleukin-1 (IL-1) Inhibits Growth of Cytomegalovirus in Human Marrow Stromal Cells: Inhibition Is Reversed Upon Removal of IL-1
Blood,
July 15, 1999;
94(2):
572 - 578.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
