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Opposite sorting of tissue factor in human umbilical vein endothelial cells
and Madin-Darby canine kidney epithelial cells
E Camerer, S Pringle, AH Skartlien, M Wiiger, K Prydz, AB Kolsto and H Prydz
Biotechnology Centre of Oslo, University of Oslo, Norway.
Tissue factor (TF) is a 48-kD transmembrane glycoprotein that triggers the
extrinsic pathway of blood coagulation by interacting with the plasma
coagulation factor VII (FVII). TF is also a true receptor in that a
cellular signal is generated when activated FVII (FVIIa) binds to TF. For
both of these functions, the cellular surface distribution of TF is
important, since FVII is primarily available on the apical side of vascular
endothelial cells and on the basolateral side of epithelial cells lining
the internal and external surfaces. We show that in endothelial cells, TF
(both antigen and procoagulant activity) is sorted to the apical surface,
whereas in wild-type and stably transfected Madin-Darby canine kidney
epithelial cells (MDCK), which form tight junctions and express TF
constitutively, TF antigen is on the basolateral surface. No significant
clotting activity is detectable on this surface. Truncated TF (cytoplasmic
tail residues 246 to 263 deleted) is sorted as wild-type in MDCK cells.
Volume 88,
Issue 4,
pp. 1339-1349,
08/15/1996
Copyright © 1996 by The American Society of Hematology
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