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Bcl-x rather than Bcl-2 mediates CD40-dependent centrocyte survival in the
germinal center
JM Tuscano, KM Druey, A Riva, J Pena, CB Thompson and JH Kehrl
Laboratory of Immunoregulation, National Institutes of Allergy and
Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-
1876, USA.
Both rapid B-cell proliferation and programmed cell death (PCD) occur
during the differentiation and selection of B cells within the germinal
center. To help elucidate the role of Bcl-x in B-cell antigen selection and
PCD within the germinal center, we examined its expression in defined
B-cell populations and by immunochemistry of tonsil tissue. Purified B-cell
fractions enriched for centrocytes express high amounts of Bcl-x and
relatively low amounts of Bcl-2, whereas fractions enriched for
centroblasts lack significant levels of both proteins. Consistent with this
observation, immunocytochemistry localized Bcl-x within cells scattered
throughout the germinal center. Stimulation of tonsil B cells with either
CD40 or Staphylococcus aureus Cowan increase bcl-x mRNA and protein levels.
Treatment of a cell line with a germinal center phenotype (RAMOS) or the
tonsillar B-cell centroblast fraction with CD40 rapidly increased Bcl-x
levels and partially rescued B cells from PCD. These data suggest that
Bcl-x rather than Bcl-2 may rescue centrocytes during selection in the
germinal center.
Volume 88,
Issue 4,
pp. 1359-1364,
08/15/1996
Copyright © 1996 by The American Society of Hematology
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