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The CD43 130-kD peripheral T-cell activation antigen is downregulated in
thymic positive selection
LG Ellies, W Tao, W Fellinger, HS Teh and HJ Ziltener
Biomedical Research Centre, University of British Columbia, Vancouver,
Canada.
Specific glycoforms of CD43, the major O-glycosylated cell-surface protein
on T lymphocytes, can affect cell adhesion according to the types of
carbohydrate side chains carried. In the peripheral immune system, CD43 130
kD, which carries core 2 O-glycan structures on its surface, is an
activation antigen expressed on both CD4 and CD8 single- positive (SP) T
cells. We have previously shown that the 115-kD resting and 130-kD
activation glycoforms of murine CD43 are differentially regulated on
peripheral SP T cells. In this study, we used transgenic mice expressing
T-cell receptors (TCRs) specific for antigens presented by class I and
class II major histocompatibility complex (MHC) molecules to determine
whether CD43 glycoforms are involved in thymocyte differentiation. Positive
selection in these mice results in an increase in the production of CD8 and
CD4 SP T cells, respectively, which express the transgenic TCR. Positive
selection is also accompanied by the upregulation of TCR, CD69, and CD5.
Using these markers to define stages of thymocyte maturation, we found that
CD43 130 kD was downregulated in the positive selection of CD4 CD8 double-
positive thymocytes expressing a class I but not class II MHC- restricted
TCR. These data suggest that core 2 glycosyltransferase (C2GnT) modulated
expression of CD43 glycoforms may be involved in thymic selection events.
Volume 88,
Issue 5,
pp. 1725-1732,
09/01/1996
Copyright © 1996 by The American Society of Hematology
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