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A unique gene encodes spliceoforms of the B-cell adhesion molecule cell
surface glycoprotein of epithelial cancer and of the Lutheran blood group
glycoprotein
C Rahuel, C Le Van Kim, MG Mattei, JP Cartron and Y Colin
INSERM U76, GIP-Institut National de la Transfusion Sanguine, Paris,
France.
Two new members of the Ig superfamily, the Lutheran (Lu) blood group
glycoprotein and the B-cell adhesion molecule (B-CAM) epithelial cancer
antigen, have been recently cloned from human placenta and colon cancer
HT29 cell line, respectively. Although amino acid sequences deduced from
cDNA analysis suggested that B-CAM should represent an abridged form of the
Lu glycoprotein lacking the last 40 amino acids of the putative cytoplasmic
tail, the relationship between the genes encoding these polypeptides has
not been determined. In the present report, we showed by Southern blot
analysis that the Lu and B-CAM cDNAs derived from a unique LU gene which
exhibited an HindIII RFLP associated with the Lua/Lub blood group
polymorphism. Accordingly, in situ hybridization of the Lu cDNA probe
confirmed the localization of the Lutheran blood group locus to chromosome
19 q13.2-13.3, as previously shown for a B-CAM DNA probe. Sequence
comparison between cDNA and genomic PCR fragments indicated that the Lu and
B-CAM transcripts previously isolated are generated through the alternative
use of internal splice donor and acceptor sites within an exon located at
the 3' end of the LU gene. These spliceoforms corresponded to 2.5 kb and
4.0 kb mRNA species detectable by Northern blot in all tissues and cell
lines in which the LU gene is expressed; their primary structures are
consistent with the presence of both the Lu and B-CAM antigens on two
glycoprotein isoforms. However, the 4.0 kb transcript was very poorly
expressed as compared to the 2.5 kb species except in the colon carcinoma
HT29 cell line, suggesting a differential regulation of the Lu/B-CAM
messenger RNA in some tumor tissues.
Volume 88,
Issue 5,
pp. 1865-1872,
09/01/1996
Copyright © 1996 by The American Society of Hematology
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