|
|
 Previous Article | Table of Contents | Next Article 
Expression of Fas/CD95 and Bcl-2 by primitive hematopoietic progenitors
freshly isolated from human fetal liver
A Barcena, SW Park, B Banapour, MO Muench and E Mechetner
Ingenex, Inc., Menlo Park, CA, USA.
The cell-surface expression and the functional status of the CD95/Fas
antigen on primitive hematopoietic progenitors (PHPs) freshly isolated from
human fetal liver (FL) were studied. PHPs were phenotypically defined as
CD34++ CD38 -/+ cells. The most immature subfractions of PHPs, CD34++CD38-
and CD34+2CD38+ FL cells, expressed CD95, whereas the more mature
CD34++CD38++ and CD34+CD38++2 FL cells displayed low CD95 expression.
Combinations of cytokines, such as kit ligand (KL) + interleukin-3 or KL +
granulocyte-macrophage colony-stimulating factor (GM-CSF) upregulated the
expression of CD95 on PHPs upon in vitro culture. Tumor necrosis
factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) further increased
the CD95 expression induced by KL+GM-CSF. The hematopoietic potential of
sorted CD34++lineage (lin)- CD95+ versus CD34++ lin-CD95-FL cells was
compared by colony-forming unit-culture (CFU-C) assays performed in
serum-deprived medium. Lin+ cells were composed of erythrocytes, monocytes,
T cells, B cells, and natural killer cells. Our results indicated that both
CD95- and CD95+ subsets contained pluripotent progenitors, generating
myeloid and erythroid progenitors. The functional status of CD95 and the
effects of TNF-alpha and IFN-gamma, cytokines known to induce CD95-mediated
apoptosis, were analyzed by incubation of PHPs in the presence of anti-CD95
monoclonal antibodies (MoAbs). The effect of anti-CD95 MoAbs was measured
by viable cell counting, flow cytometry, and CFU-C assays. A decrease of
CFU-C numbers was observed in the presence of anti-CD95 MoAbs and TNF-
alpha and/or IFN-gamma. However, whereas growth factor deprivation induced
apoptosis of PHPs, cross-linking of CD95 did not lead to apoptosis of PHPs
measured by flow cytometry and viable cell counting. The correlation of
increased intracytoplasmic levels of bcl-2 with high levels of cell-surface
CD34 and the presence of CD95 on fresh FL cells suggests that bcl-2 may be
involved in protecting against CD95-mediated apoptosis of FL PHPs.
Volume 88,
Issue 6,
pp. 2013-2025,
09/15/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Barcena, M. O. Muench, M. Kapidzic, and S. J. Fisher
A New Role for the Human Placenta as a Hematopoietic Site Throughout Gestation
Reproductive Sciences,
February 1, 2009;
16(2):
178 - 187.
[Abstract]
[PDF]
|
|
|
|
|
|
|
Y.-E. Claessens, M. Fontenay, F. Pene, J.-D. Chiche, M. Guesnu, C. Hababou, N. Casadevall, J.-F. Dhainaut, J.-P. Mira, and A. Cariou
Erythropoiesis Abnormalities Contribute to Early-Onset Anemia in Patients with Septic Shock
Am. J. Respir. Crit. Care Med.,
July 1, 2006;
174(1):
51 - 57.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Dybedal, L. Yang, D. Bryder, I. Aastrand-Grundstrom, K. Leandersson, and S. E. W. Jacobsen
Human reconstituting hematopoietic stem cells up-regulate Fas expression upon active cell cycling but remain resistant to Fas-induced suppression
Blood,
July 1, 2003;
102(1):
118 - 126.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-J. Lataillade, D. Clay, P. Bourin, F. Herodin, C. Dupuy, C. Jasmin, and M.-C. Le Bousse-Kerdiles
Stromal cell-derived factor 1 regulates primitive hematopoiesis by suppressing apoptosis and by promoting G0/G1 transition in CD34+ cells: evidence for an autocrine/paracrine mechanism
Blood,
February 15, 2002;
99(4):
1117 - 1129.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Bryder, V. Ramsfjell, I. Dybedal, K. Theilgaard-Monch, C.-M. Hogerkorp, J. Adolfsson, O. J. Borge, and S. E. W. Jacobsen
Self-Renewal of Multipotent Long-Term Repopulating Hematopoietic Stem Cells Is Negatively Regulated by FAS and Tumor Necrosis Factor Receptor Activation
J. Exp. Med.,
October 1, 2001;
194(7):
941 - 952.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Laurent and J.-P. Jaffrezou
Signaling pathways activated by daunorubicin
Blood,
August 15, 2001;
98(4):
913 - 924.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Moreau, M. Leite-de-Moraes, S. Ezine, J. P. Di Santo, M. Dy, and E. Schneider
Natural killer cell-dependent apoptosis of peripheral murine hematopoietic progenitor cells in response to Fas cross-linking: involvement of tumor necrosis factor-{alpha}
Blood,
May 15, 2001;
97(10):
3069 - 3074.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Silvestris, M. Tucci, P. Cafforio, and F. Dammacco
Fas-L up-regulation by highly malignant myeloma plasma cells: role in the pathogenesis of anemia and disease progression
Blood,
March 1, 2001;
97(5):
1155 - 1164.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Schneider, G. Moreau, A. Arnould, F. Vasseur, N. Khodabaccus, M. Dy, and S. Ezine
Increased Fetal and Extramedullary Hematopoiesis in Fas-Deficient C57BL/6-lpr/lpr Mice
Blood,
October 15, 1999;
94(8):
2613 - 2621.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Jenkins, M. Keir, and J. M. McCune
Fas Is Expressed Early in Human Thymocyte Development But Does Not Transmit an Apoptotic Signal
J. Immunol.,
August 1, 1999;
163(3):
1195 - 1204.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. De Maria, U. Testa, L. Luchetti, A. Zeuner, G. Stassi, E. Pelosi, R. Riccioni, N. Felli, P. Samoggia, and C. Peschle
Apoptotic Role of Fas/Fas Ligand System in the Regulation of Erythropoiesis
Blood,
February 1, 1999;
93(3):
796 - 803.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Halaas, R. Vik, and T. Espevik
Induction of Fas Ligand in Murine Bone Marrow NK Cells by Bacterial Polysaccharides
J. Immunol.,
May 1, 1998;
160(9):
4330 - 4336.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Moses, J. Nelson, and G. C. Bagby Jr
The Influence of Human Immunodeficiency Virus-1 on Hematopoiesis
Blood,
March 1, 1998;
91(5):
1479 - 1495.
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-H. Dai, J. O. Price, T. Brunner, and S. B. Krantz
Fas Ligand Is Present in Human Erythroid Colony-Forming Cells and Interacts With Fas Induced by Interferon gamma to Produce Erythroid Cell Apoptosis
Blood,
February 15, 1998;
91(4):
1235 - 1242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Iijima, K. Miyamura, T. Itou, M. Tanimoto, R. Sobue, and H. Saito
Functional Expression of Fas (CD95) in Acute Myeloid Leukemia Cells in the Context of CD34 and CD38 Expression: Possible Correlation With Sensitivity to Chemotherapy
Blood,
December 15, 1997;
90(12):
4901 - 4909.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Rathbun, G. R. Faulkner, M. H. Ostroski, T. A. Christianson, G. Hughes, G. Jones, R. Cahn, R. Maziarz, G. Royle, W. Keeble, et al.
Inactivation of the Fanconi Anemia Group C Gene Augments Interferon-gamma -Induced Apoptotic Responses in Hematopoietic Cells
Blood,
August 1, 1997;
90(3):
974 - 985.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
