|
|
 Previous Article | Table of Contents | Next Article 
A variant CD30 protein lacking extracellular and transmembrane domains is
induced in HL-60 by tetradecanoylphorbol acetate and is expressed in
alveolar macrophages
R Horie, K Ito, M Tatewaki, M Nagai, S Aizawa, M Higashihara, T Ishida, J Inoue, H Takizawa and T Watanabe
Department of Pathology, University of Tokyo, Japan.
We identified and cloned cDNAs for two novel CD30 mRNAs of 2.3 kb that are
induced by 12-O-tetradecanoylphorbol 13-acetate (TPA) in the human myeloid
leukemia cell line HL-60. These transcripts were transcribed from the
intronic region just upstream of the exon coding for the transmembrane
domain of the CD30 protein. The shorter cDNA had a deletion of 54
nucleotides corresponding to the 3' region of the transmembrane domain of
the CD30 and which was probably caused by alternative splicing. Translation
of these transcripts appeared to start from the internal methionine codon
at nucleotide position 289 that corresponds to that of 1612 in the CD30
cDNA, and encode a protein of 132 amino acid residues which corresponds
exactly to the C-terminal cytoplasmic domain of CD30 protein. The
calculated molecular mass of this variant CD30 (CD30v) protein was 14,087.
Thus, the predicted CD30v protein retains most of the cytoplasmic region,
but lacks the extracellular and transmembrane domains. Northern blots
detected the expression of CD30v transcripts only in the lung and the
TPA-stimulated HL-60 cell line. Translation of this mRNA in vitro produced
a protein of 25 kD. Immunoblotting analysis with HCD30C1, a rabbit
polyclonal antibody raised against the cytoplasmic domain of CD30 protein,
detected proteins with an apparent Mr 25 kD expressed in TPA-stimulated
HL-60 and COS-7 cells that were transfected with both types of CD30v cDNAs.
Constitutive phosphorylation of the CD30v protein was demonstrated by in
vitro labeling with [32P]. Immunohistochemical studies demonstrated CD30v
protein was in alveolar macrophages. Cotransfection experiments using a
kappa B-site-dependent reporter construct showed that CD30v can
transactivate gene expression through activation of NF kappa B, as was
noted on the authentic CD30 protein. Overexpression of the CD30v induced
differentiation of HL-60 cells as evidenced by an increased NBT reduction
activity. These observations provided new insights into the molecular
heterogeneity and biological function of CD30 in myeloid cells.
Volume 88,
Issue 7,
pp. 2422-2432,
10/01/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Watanabe, Y. Ogawa, K. Ito, M. Higashihara, M. E. Kadin, L. J. Abraham, T. Watanabe, and R. Horie
AP-1 Mediated Relief of Repressive Activity of the CD30 Promoter Microsatellite in Hodgkin and Reed-Sternberg Cells
Am. J. Pathol.,
August 1, 2003;
163(2):
633 - 641.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Cerutti, A. Schaffer, R. G. Goodwin, S. Shah, H. Zan, S. Ely, and P. Casali
Engagement of CD153 (CD30 Ligand) by CD30+ T Cells Inhibits Class Switch DNA Recombination and Antibody Production in Human IgD+ IgM+ B Cells
J. Immunol.,
July 15, 2000;
165(2):
786 - 794.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Horie, V. Gattei, K. Ito, S. Imajo-Ohmi, T. Tange, J. Miyauchi, A. Pinto, M. Degan, A. De Iuliis, F. Tassan Mazzocco, et al.
Frequent Expression of the Variant CD30 in Human Malignant Myeloid and Lymphoid Neoplasms
Am. J. Pathol.,
December 1, 1999;
155(6):
2029 - 2041.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
