Granulocyte colony-stimulating factor mobilizes into peripheral blood the
complete clonal repertoire of hematopoietic precursors residing in the bone
marrow of mice
F Varas, A Bernad and JA Bueren
Unidad de Biologia Molecular y Celular, CIEMAT, Madrid, Spain.
We have established the clonal relationships between the hematopoietic
precursors residing in the bone marrow (BM) and the peripheral blood (PB)
of mice treated with granulocyte colony-stimulating factor (G- CSF). The
use of animals whose hematopoiesis was reconstituted with genetically
labeled stem cells has allowed us to show that an almost identical
repertoire of clones is found in the colony-forming unit (CFU- S)
population present in the BM and mobilized PB. Moreover, our data has shown
that the frequency of expression of the repopulating clones in both types
of CFU-S populations is the same, evidencing that G-CSF mobilized PB
progenitor cells (PBPCs) closely reflect the clonal make- up of the
hematopoietic precursors residing in the BM. When secondary recipients were
transplanted with BM or mobilized PB grafts that had been harvested from
the genetically marked mice, the presence of long- term
lympho-hematopoietic repopulating clones was showed not only in the BM but
also in the PB samples. No new clones were identified in the long-term
repopulating cells of the mobilized animals with respect to those found in
the CFU-S population. Moreover, the hematopoietic precursors that were
capable of long-term reconstitution corresponded to the clones, which were
most highly represented in the CFU-S compartment, suggesting, at least in
the case of G-CSF treated mice, that the frequency of expression of the
repopulating clones in the CFU- S population is prognostic for the clone
longevity. Based on our experimental data, new advantages for the use of
mobilized PBPCs in place of hematopoietic grafts procured from limited
areas of BM are proposed.
Volume 88,
Issue 7,
pp. 2495-2501,
10/01/1996
Copyright © 1996 by The American Society of Hematology
