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CN Berger and KS Sturm
Department Forschung, Kantonsspital Basel, Switzerland.
Differentiation of hematopoietic precursor cells results in the formation
of clonally related descendent cells. Using the mosaic expression of
beta-galactosidase in female mouse fetuses heterozygous for an X-linked
lacZ transgene, we analyzed the clonal relationship of the hematopoietic
progeny. The proportion of beta-galactosidase positive cells for different
T- and B-lymphoid and myeloid cell populations was determined at different
stages of fetal development. We found excellent correlations of the
proportion of beta-galactosidase expressing cells for all hematopoietic
lineages confirming that they share a common ancestry. Therefore, it was
possible to estimate the number of common precursor cells (PC) based on
binomial distribution and covariance analysis of pairs of different
hematopoietic cell populations. Our results obtained from hematopoietic
cells at 15.5 to 18.5 days of gestation indicated the presence of 15 to 18
lymphoid and 18 to 22 myeloid/lymphoid specific precursor cells.
Statistical analysis of the precursor cell numbers showed a trend of
increasing numbers that was highly significant. The precursor cell number
was inversely related to maturity of the cell populations analyzed; ie, the
lowest number of lymphoid and lymphoid/myeloid precursors was calculated
when the most mature CD3+ T-cell population was used for comparison.
Determination of PC numbers can therefore be used to assess the relative
maturity and developmental potential of individual cell populations.
This article has been cited by other articles:
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| Copyright © 1996 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
