|
|
 Previous Article | Table of Contents | Next Article 
Mobilization of CD34+ progenitor cells by granulocyte colony- stimulating
factor in human immunodeficiency virus type 1-infected adults
KS Slobod, TA Bennett, PJ Freiden, AM Kechli, N Howlett, PM Flynn, DR Head, DK Srivastava, JM Boyett, MK Brenner and JV Garcia
Department of Infectious Diseases, St Jude Children's Research Hospital,
Memphis, TN 38105, USA.
We conducted a clinical trial to determine the feasibility of growth factor
mobilization of CD34+ progenitor cells in human immunodeficiency virus type
1 (HIV-1)-infected individuals. Eight asymptomatic, HIV-1- infected adults
(median CD4+ T-cell count, 415 cells/microL), received 480 micrograms/d of
granulocyte colony-stimulating factor (G-CSF) for 6 days without evidence
of viral activation. Despite concerns that HIV-1 might inhibit
hematopoiesis, CD34+ cells were successfully mobilized to the periphery of
all donors, independent of the baseline CD4+ T-cell count, and the status
of antiretroviral therapy. Leukapheresis was performed on day 6, and
yielded a median of 194 x 10(6) CD34+ cells per leukapheresis (n = 7).
CD34-enriched cells from the leukapheresis were predominantly
myeloid-committed, but between 0.2% and 1.7% were primitive CD34+/CD38-
progenitors. A median of 21.7% of the mobilized CD34+ cells were dimly
positive for CD4. Consequently, CD34(+)-enriched cells were purified on the
cell sorter (mean purity, 97.7% +/- 2.4%; n = 7), and examined for HIV-1
DNA. Purified CD34+ cells from two of seven donors were polymerase chain
reaction (PCR)-positive for HIV-1, but only from one of three samples from
each donor. We conclude that G- CSF can safely mobilize CD34+ progenitor
cells in HIV-1-infected subjects, and that these cells are suitable for
consideration in gene- transfer strategies.
Volume 88,
Issue 9,
pp. 3329-3335,
11/01/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
G. Romano, P. Micheli, C. Pacilio, and A. Giordano
Latest Developments in Gene Transfer Technology: Achievements, Perspectives, and Controversies over Therapeutic Applications
Stem Cells,
January 1, 2000;
18(1):
19 - 39.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. B. Campbell, A. Sevin, R. W. Coombs, G. C. Peterson, M. Rosandich, D. R. Kuritzkes, J. Mladenovic, A. Landay, R. Wong, D. Ambruso, et al.
Changes in human immunodeficiency virus type 1 virus load during mobilization and harvesting of hemopoietic progenitor cells
Blood,
January 1, 2000;
95(1):
48 - 55.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Aiuti, L. Turchetto, M. Cota, A. Cipponi, A. Brambilla, C. Arcelloni, R. Paroni, E. Vicenzi, C. Bordignon, and G. Poli
Human CD34+ Cells Express CXCR4 and Its Ligand Stromal Cell-Derived Factor-1. Implications for Infection by T-Cell Tropic Human Immunodeficiency Virus
Blood,
July 1, 1999;
94(1):
62 - 73.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Moses, J. Nelson, and G. C. Bagby Jr
The Influence of Human Immunodeficiency Virus-1 on Hematopoiesis
Blood,
March 1, 1998;
91(5):
1479 - 1495.
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Junker, J. J. Moon, C. S. Kalfoglou, I. Sniecinski, S. J. Forman, J. A. Zaia, H. Kaneshima, and E. Bohnlein
Hematopoietic Potential and Retroviral Transduction of CD34+Thy-1+ Peripheral Blood Stem Cells From Asymptomatic Human Immunodeficiency Virus Type-1-Infected Individuals Mobilized With Granulocyte Colony-Stimulating Factor
Blood,
June 15, 1997;
89(12):
4299 - 4306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
