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Functional comparison of spleen dendritic cells and dendritic cells
cultured in vitro from bone marrow precursors
K Garrigan, P Moroni-Rawson, C McMurray, I Hermans, N Abernethy, J Watson and F Ronchese
Malaghan Institute of Medical Research, Wellington South, New Zealand.
We have compared dendritic cells (DC) isolated from mouse spleen, or
generated in vitro from bone marrow (BM) precursors cultured in granulocyte
macrophage-colony stimulating factor (GM-CSF) and interleukin-4 (IL-4), for
the ability to process and present soluble antigen and stimulate major
histocompatibility complex (MHC) Class II- restricted T cells. DC from
spleen or BM cultures were equally able to stimulate the in vitro
proliferation of allogeneic T cells or of antigen-specific T-cell receptor
(TCR)-transgenic T cells. Both DC populations also induced comparable
levels of IL-2 secretion by a T- cell hybridoma. Therefore, splenic and
BM-derived DC express comparable levels of (Antigen + MHC Class II) ligands
and/or costimulatory molecules and have comparable ability to stimulate
T-cell responses. When presentation of a native protein antigen, rather
than peptide, was evaluated, BM-derived DC were at least 50 times better
than splenic DC at stimulating the proliferation of TCR-transgenic T cells.
The antigen processing ability of the two populations was similar only when
splenic DC were used immediately ex vivo. Therefore, unlike spleen DC, BM-
derived DC maintain the capacity to process protein antigen for MHC Class
II presentation during in vitro culture. Due to these characteristics,
BM-derived DC may represent a useful tool in immunotherapy studies, as they
combine high T-cell stimulatory properties with the capacity to process and
present native antigen.
Volume 88,
Issue 9,
pp. 3508-3512,
11/01/1996
Copyright © 1996 by The American Society of Hematology
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