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Localization and regulation of thrombopoietin mRNa expression in human
kidney, liver, bone marrow, and spleen using in situ hybridization
R Sungaran, B Markovic and BH Chong
Department of Haematology, Prince of Wales Hospital/school of Pathology,
Sydney, Australia.
Thrombopoietin (TPO) is the primary hematopoietic growth factor involved in
the regulation of platelet production. Although the kidney, liver, bone
marrow (BM), and spleen have been identified as the major sources of TPO
production, the precise cellular location of TPO mRNA expression in these
tissues remains unknown. We have identified the cells expressing TPO mRNA
in the human kidney, liver, and BM using an in situ hybridization assay. In
the BM of individuals with normal platelet counts, the hybridization signal
was too weak to allow identification of the TPO mRNA expressing cells.
However, in thrombocytopenic subjects with aplastic anemia,
postchemotherapy marrow aplasia, and immune thrombocytopenia, the stromal
cells showed strong TPO mRNA expression. In the human subjects with normal
platelet counts, the cells of the proximal convoluted tubules of the kidney
showed consistent positive staining whereas the signal in the cells of the
distal convoluted tubules was less consistent. Strong hybridization signal
was also evident in the hepatocytes. The hybridization signal in the
spleen, even in thrombocytopenic subjects, was too weak to allow confident
identification of the cells expressing TPO mRNA. In all subjects, the
interstitial cells and endothelial cells of the liver and spleen, the renal
peritubular cells, and the hematopoietic precursor cells of the BM showed
no TPO mRNA expression. Our data suggest that TPO mRNA expression in the
human BM may be modulated by platelet mass.
Volume 89,
Issue 1,
pp. 101-107,
01/01/1997
Copyright © 1997 by The American Society of Hematology
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|
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|
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|
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