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Identification of a serpin specifically expressed in multipotent and
bipotent hematopoietic progenitor cells and in activated T cells
IN Hampson, L Hampson, M Pinkoski, M Cross, CM Heyworth, RC Bleackley, E Atkinson and TM Dexter
CRC Department of Experimental Haematology, Paterson Institute of Cancer
Research, Christie Hospital, Manchester, UK.
We have identified a gene that has a high level of mRNA expression in
undifferentiated, multipotential hematopoietic cells (FDCP-Mix) and that
downregulates both transcript and protein, as these cells are induced to
differentiate into mature myeloid cells. Sequence analysis of this gene has
identified it as a serine protease inhibitor EB22/3 (serpin 2A).
Constitutive expression of serpin 2A in FDCP-Mix cells was associated with
an increase in the clonogenic potential of the cells and with a delay in
the appearance of fully mature cells in cultures undergoing granulocyte
macrophage differentiation when compared with control cells. Serpin 2A was
also found to be expressed in bone marrow- derived bipotent granulocyte
macrophage progenitor cells (GM-colony forming cell [CFC]), but not in
erythrocyte progenitor cells from day 15 fetal liver. Expression of serpin
2A also showed a marked up regulation during the activation of cytotoxic
suppressor CD8+ T cells, with a clear lag between the appearance of
transcript and detection of protein.
Volume 89,
Issue 1,
pp. 108-118,
01/01/1997
Copyright © 1997 by The American Society of Hematology
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