|
|
 Previous Article | Table of Contents | Next Article 
Isolation of a T-cell clone showing HLA-DRB1*0405-restricted cytotoxicity
for hematopoietic cells in a patient with aplastic anemia
S Nakao, A Takami, H Takamatsu, W Zeng, N Sugimori, H Yamazaki, Y Miura, M Ueda, S Shiobara, T Yoshioka, T Kaneshige, M Yasukawa and T Matsuda
Third Department of Medicine, Kanazawa University School of Medicine,
Ishikawa, Japan.
The existence of T cells capable of inhibiting in vitro hematopoiesis has
been shown in aplastic anemia (AA), although whether such inhibition is
mediated by a specific immune reaction involving an HLA allele remained
unknown. We isolated a CD4+ Vbeta21+ T-cell clone that was most dominant
among Vbeta21+ T cells in the bone marrow (BM) of an AA patient whose
HLA-DRB1 alleles included 1501 and 0405. The T-cell clone named NT4.2 lysed
an autologous Epstein-Barr virus-transformed lymphoblastoid cell line (LCL)
and phytohemagglutinin-stimulated lymphocytes (PHA-blasts) as well as
allogeneic LCLs sharing HLA- DRB1*0405. Cytotoxicity against LCL cells and
PHA-blasts by NT4.2 was blocked by anti-HLA-DR monoclonal antibody (MoAb)
or anti-CD3 MoAb. NT4.2 also lysed autologous BM mononuclear cells enriched
with CD34+ cells that had been cultured for one week in the presence of
colony- stimulating factors as well as allogeneic CD34+ cells of a normal
individual carrying HLA-DRB1*0405, cultured in the same way. Moreover,
NT4.2 strongly inhibited colony formation by hematopoietic progenitor cells
derived from cultured CD34+ cells sharing HLA-DRB1*0405. These results
indicate that the AA patient has T cells capable of killing hematopoietic
cells in an HLA-DRB1*0405-restricted manner and that such cytotoxic T cells
may contribute to the pathogenesis of AA.
Volume 89,
Issue 10,
pp. 3691-3699,
05/15/1997
Copyright © 1997 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
H. Takamatsu, J. L. Espinoza, X. Lu, Z. Qi, K. Okawa, and S. Nakao
Anti-Moesin Antibodies in the Serum of Patients with Aplastic Anemia Stimulate Peripheral Blood Mononuclear Cells to Secrete TNF-{alpha} and IFN-{gamma}
J. Immunol.,
January 1, 2009;
182(1):
703 - 710.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. W. Wlodarski, L. P. Gondek, Z. P. Nearman, M. Plasilova, M. Kalaycio, E. D. Hsi, and J. P. Maciejewski
Molecular strategies for detection and quantitation of clonal cytotoxic T-cell responses in aplastic anemia and myelodysplastic syndrome
Blood,
October 15, 2006;
108(8):
2632 - 2641.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Fureder, M.-T. Krauth, W. R. Sperr, K. Sonneck, I. Simonitsch-Klupp, L. Mullauer, M. Willmann, H.-P. Horny, and P. Valent
Evaluation of Angiogenesis and Vascular Endothelial Growth Factor Expression in the Bone Marrow of Patients with Aplastic Anemia
Am. J. Pathol.,
January 1, 2006;
168(1):
123 - 130.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Zeng, A. Miyazato, G. Chen, S. Kajigaya, N. S. Young, and J. P. Maciejewski
Interferon-{gamma}-induced gene expression in CD34 cells: identification of pathologic cytokine-specific signature profiles
Blood,
January 1, 2006;
107(1):
167 - 175.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Feng, T. Chuhjo, C. Sugimori, T. Kotani, X. Lu, A. Takami, H. Takamatsu, H. Yamazaki, and S. Nakao
Diazepam-binding inhibitor-related protein 1: a candidate autoantigen in acquired aplastic anemia patients harboring a minor population of paroxysmal nocturnal hemoglobinuria-type cells
Blood,
October 15, 2004;
104(8):
2425 - 2431.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Zeng, G. Chen, S. Kajigaya, O. Nunez, A. Charrow, E. M. Billings, and N. S. Young
Gene expression profiling in CD34 cells to identify differences between aplastic anemia patients and healthy volunteers
Blood,
January 1, 2004;
103(1):
325 - 332.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Hirano, M. O. Butler, M. S. von Bergwelt-Baildon, B. Maecker, J. L. Schultze, K. C. O'Connor, P. H. Schur, S. Kojima, E. C. Guinan, and L. M. Nadler
Autoantibodies frequently detected in patients with aplastic anemia
Blood,
December 15, 2003;
102(13):
4567 - 4575.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Murakami, H. Kosaka, Y. Maeda, J.-i. Nishimura, N. Inoue, K. Ohishi, M. Okabe, J. Takeda, and T. Kinoshita
Inefficient response of T lymphocytes to glycosylphosphatidylinositol anchor-negative cells: implications for paroxysmal nocturnal hemoglobinuria
Blood,
December 1, 2002;
100(12):
4116 - 4122.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Risitano, H. Kook, W. Zeng, G. Chen, N. S. Young, and J. P. Maciejewski
Oligoclonal and polyclonal CD4 and CD8 lymphocytes in aplastic anemia and paroxysmal nocturnal hemoglobinuria measured by Vbeta CDR3 spectratyping and flow cytometry
Blood,
June 17, 2002;
100(1):
178 - 183.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kook, A. M. Risitano, W. Zeng, M. Wlodarski, C. Lottemann, R. Nakamura, J. Barrett, N. S. Young, and J. P. Maciejewski
Changes in T-cell receptor VB repertoire in aplastic anemia: effects of different immunosuppressive regimens
Blood,
May 15, 2002;
99(10):
3668 - 3675.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Brodsky, L. L. Sensenbrenner, B. D. Smith, D. Dorr, P. J. Seaman, S. M. Lee, J. E. Karp, I. Brodsky, and R. J. Jones
Durable Treatment-Free Remission after High-Dose Cyclophosphamide Therapy for Previously Untreated Severe Aplastic Anemia
Ann Intern Med,
October 2, 2001;
135(7):
477 - 483.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Miura, C. J. Thoburn, E. C. Bright, M. Sommer, S. Lefell, M. Ueda, S. Nakao, and A. D. Hess
Characterization of the T-cell repertoire in autologous graft-versus-host disease (GVHD): evidence for the involvement of antigen-driven T-cell response in the development of autologous GVHD
Blood,
August 1, 2001;
98(3):
868 - 876.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Zeng, S. Nakao, H. Takamatsu, A. Yachie, A. Takami, Y. Kondo, N. Sugimori, H. Yamazaki, Y. Miura, S. Shiobara, et al.
Characterization of T-Cell Repertoire of the Bone Marrow in Immune-Mediated Aplastic Anemia: Evidence for the Involvement of Antigen-Driven T-Cell Response in Cyclosporine-Dependent Aplastic Anemia
Blood,
May 1, 1999;
93(9):
3008 - 3016.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Hasegawa, S. Kojima, E. Tatsumi, A. Hayakawa, Y. Kosaka, H. Nakamura, M. Sako, Y. Osugi, S. Nagata, and K. Sano
Elevation of the Serum Fas Ligand in Patients With Hemophagocytic Syndrome and Diamond-Blackfan Anemia
Blood,
April 15, 1998;
91(8):
2793 - 2799.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
