|
|
 Previous Article | Table of Contents | Next Article 
Neutrophils can adhere via alpha4beta1-integrin under flow conditions
PH Reinhardt, JF Elliott and P Kubes
Immunology Research Group, University of Calgary, Alberta, Canada.
In this study we investigated the possibility that an alternative pathway
exists for neutrophil recruitment, namely an alpha4beta1- dependent
pathway. A parallel plate chamber was used to investigate whether
neutrophils could tether, roll, and adhere to tumor necrosis factor alpha
(TNF alpha)-stimulated endothelium via alpha4beta1. alpha4beta1-integrin
was induced on neutrophils using dihydrocytochalasin B and either an
endogenous (endothelial-derived) chemotactic agent or an exogenous
chemotactic molecule. alpha4beta1- expressing neutrophils could stably
adhere under shear force (2 dyne/cm2) to TNF alpha-stimulated endothelium
independent of the beta2- integrin. The firm adhesion was entirely
abolished by antibodies directed against either the alpha4 or
beta1-integrin subunits. However, the rolling interaction was not dependent
on alpha4beta1 but was abolished by antiselectin therapy. Neutrophils
expressing alpha4beta1 could also tether to the endothelium in the presence
of antiselectin therapy, but at shear stresses less than 2 dyne/cm2.
alpha4beta1- expressing neutrophils also tethered to and stably adhered (no
rolling) to VCAM-1- but not to ICAM-1-transfected L cells. The interaction
only occurred at shear stress less than 2 dyne/cm2. A cell line (Ramos)
known to express high quantities of alpha4beta1-integrin interacted with
VCAM-1-transfected L cells at very similar shear conditions.
alpha4beta1-expressing neutrophils were also able to adhere to a second
alpha4-integrin ligand, fibronectin; however, this interaction only
occurred under static conditions. These data suggest that, under certain
conditions, neutrophils can adhere independently of the beta2- integrin
pathway and adhere via the alpha4beta1-integrin. This study refutes the
concept that alpha4beta1-integrin adhesion is restricted to mononuclear
leukocytes and is not functional on human neutrophils.
Volume 89,
Issue 10,
pp. 3837-3846,
05/15/1997
Copyright © 1997 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. P. Kuligowski, R. Y. Q. Kwan, C. Lo, C. Wong, W. G. James, D. Bourges, J. D. Ooi, L. D. Abeynaike, P. Hall, A. R. Kitching, et al.
Antimyeloperoxidase antibodies rapidly induce {alpha}4-integrin-dependent glomerular neutrophil adhesion
Blood,
June 18, 2009;
113(25):
6485 - 6494.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Petty, C. C. Lenox, D. J. Weiss, M. E. Poynter, and B. T. Suratt
Crosstalk between CXCR4/Stromal Derived Factor-1 and VLA-4/VCAM-1 Pathways Regulates Neutrophil Retention in the Bone Marrow
J. Immunol.,
January 1, 2009;
182(1):
604 - 612.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Heit, L. Liu, P. Colarusso, K. D. Puri, and P. Kubes
PI3K accelerates, but is not required for, neutrophil chemotaxis to fMLP
J. Cell Sci.,
January 15, 2008;
121(2):
205 - 214.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Zhou, B. M. Lapointe, S. R. Clark, L. Zbytnuik, and P. Kubes
A Requirement for Microglial TLR4 in Leukocyte Recruitment into Brain in Response to Lipopolysaccharide
J. Immunol.,
December 1, 2006;
177(11):
8103 - 8110.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. S. Bonder, S. R. Clark, M. U. Norman, P. Johnson, and P. Kubes
Use of CD44 by CD4+ Th1 and Th2 lymphocytes to roll and adhere
Blood,
June 15, 2006;
107(12):
4798 - 4806.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-H. Woo, J.-H. Lim, and J.-H. Kim
VCAM-1 upregulation via PKC{delta}-p38 kinase-linked cascade mediates the TNF-{alpha}-induced leukocyte adhesion and emigration in the lung airway epithelium
Am J Physiol Lung Cell Mol Physiol,
February 1, 2005;
288(2):
L307 - L316.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. L. Speyer, H. Gao, N. J. Rancilio, T. A. Neff, G. B. Huffnagle, J. V. Sarma, and P. A. Ward
Novel Chemokine Responsiveness and Mobilization of Neutrophils during Sepsis
Am. J. Pathol.,
December 1, 2004;
165(6):
2187 - 2196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Alvarez, M. Cerda-Nicolas, Y. Naim Abu Nabah, M. Mata, A. C. Issekutz, J. Panes, R. R. Lobb, and M.-J. Sanz
Direct evidence of leukocyte adhesion in arterioles by angiotensin II
Blood,
July 15, 2004;
104(2):
402 - 408.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Amersi, X.-D. Shen, C. Moore, J. Melinek, R. W. Busuttil, J. W. Kupiec-Weglinski, and A. J. Coito
Fibronectin-{alpha}4{beta}1 Integrin-Mediated Blockade Protects Genetically Fat Zucker Rat Livers from Ischemia/Reperfusion Injury
Am. J. Pathol.,
April 1, 2003;
162(4):
1229 - 1239.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. H. Wyman, A. J. Bjornsen, D. J. Elzi, C. W. Smith, K. M. England, M. Kelher, and C. C. Silliman
A two-insult in vitro model of PMN-mediated pulmonary endothelial damage: requirements for adherence and chemokine release
Am J Physiol Cell Physiol,
December 1, 2002;
283(6):
C1592 - C1603.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Gironella, M. Molla, A. Salas, A. Soriano, M. Sans, D. Closa, P. Engel, A. Salas, J. M. Pique, and J. Panes
The role of P-selectin in experimental colitis as determined by antibody immunoblockade and genetically deficient mice
J. Leukoc. Biol.,
July 1, 2002;
72(1):
56 - 64.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tasaka, S. E. Richer, J. P. Mizgerd, and C. M. Doerschuk
Very Late Antigen-4 in CD18-Independent Neutrophil Emigration during Acute Bacterial Pneumonia in Mice
Am. J. Respir. Crit. Care Med.,
July 1, 2002;
166(1):
53 - 60.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Pettersen and K. B. Adler
Airways Inflammation and COPD* : Epithelial-Neutrophil Interactions
Chest,
May 1, 2002;
121(5_suppl):
142S - 150S.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kaur, R. C. Woodman, L. Ostrovsky, and P. Kubes
Selective recruitment of neutrophils and lymphocytes by thrombin: a role for NF-{kappa}B
Am J Physiol Heart Circ Physiol,
August 1, 2001;
281(2):
H784 - H795.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Cotter, K. E. Norman, P. G. Hellewell, and V. C. Ridger
A Novel Method for Isolation of Neutrophils from Murine Blood Using Negative Immunomagnetic Separation
Am. J. Pathol.,
August 1, 2001;
159(2):
473 - 481.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. B. Henderson, L. H.K. Lim, P. A. Tessier, F. N.E. Gavins, M. Mathies, M. Perretti, and N. Hogg
The Use of Lymphocyte Function-associated Antigen (LFA)-1-deficient Mice to Determine the Role of LFA-1, Mac-1, and {alpha}4 Integrin in the Inflammatory Response of Neutrophils
J. Exp. Med.,
July 16, 2001;
194(2):
219 - 226.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Burns, T. B. Issekutz, H. Yagita, and A. C. Issekutz
The {{alpha}}4{{beta}}1 (Very Late Antigen (VLA)-4, CD49d/CD29) and {{alpha}}5{{beta}}1 (VLA-5, CD49e/CD29) Integrins Mediate {{beta}}2 (CD11/CD18) Integrin-Independent Neutrophil Recruitment to Endotoxin-Induced Lung Inflammation
J. Immunol.,
April 1, 2001;
166(7):
4644 - 4649.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Pereira, M. Zhou, A. Mocsai, and C. Lowell
Resting Murine Neutrophils Express Functional {{alpha}}4 Integrins that Signal Through Src Family Kinases
J. Immunol.,
March 15, 2001;
166(6):
4115 - 4123.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. C. Ridger, B. E. Wagner, W. A. H. Wallace, and P. G. Hellewell
Differential Effects of CD18, CD29, and CD49 Integrin Subunit Inhibition on Neutrophil Migration in Pulmonary Inflammation
J. Immunol.,
March 1, 2001;
166(5):
3484 - 3490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Briaud, Z.-M. Ding, L. H. Michael, M. L. Entman, S. Daniel, and C. M. Ballantyne
Leukocyte trafficking and myocardial reperfusion injury in ICAM-1/P-selectin-knockout mice
Am J Physiol Heart Circ Physiol,
January 1, 2001;
280(1):
H60 - H67.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Y. Larbi, A. R. Allen, F. W. K. Tam, D. O. Haskard, R. R. Lobb, P. M. R. Silva, and S. Nourshargh
VCAM-1 has a tissue-specific role in mediating interleukin-4-induced eosinophil accumulation in rat models: evidence for a dissociation between endothelial-cell VCAM-1 expression and a functional role in eosinophil migration
Blood,
November 15, 2000;
96(10):
3601 - 3609.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. Wagner and R. A. Roth
Neutrophil Migration Mechanisms, with an Emphasis on the Pulmonary Vasculature
Pharmacol. Rev.,
September 1, 2000;
52(3):
349 - 374.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kirveskari, P. Bono, K. Granfors, M. Leirisalo-Repo, S. Jalkanen, and M. Salmi
Expression of {alpha}4-integrins on human neutrophils
J. Leukoc. Biol.,
August 1, 2000;
68(2):
243 - 250.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. E. May, F.-J. Neumann, A. Schomig, and K. T. Preissner
VLA-4 (alpha 4beta 1) engagement defines a novel activation pathway for beta 2 integrin-dependent leukocyte adhesion involving the urokinase receptor
Blood,
July 15, 2000;
96(2):
506 - 513.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ali, J. Kaur, and K. D. Patel
Intercellular Cell Adhesion Molecule-1, Vascular Cell Adhesion Molecule-1, and Regulated on Activation Normal T Cell Expressed and Secreted Are Expressed by Human Breast Carcinoma Cells and Support Eosinophil Adhesion and Activation
Am. J. Pathol.,
July 1, 2000;
157(1):
313 - 321.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Ostrovsky, J. Carvalho-Tavares, R. C. Woodman, and P. Kubes
Translational inhibition of E-selectin expression stimulates P-selectin-dependent neutrophil recruitment
Am J Physiol Heart Circ Physiol,
April 1, 2000;
278(4):
H1225 - H1232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Johnston, A. Chee, T. B. Issekutz, T. Ugarova, A. Fox-Robichaud, M. J. Hickey, and P. Kubes
{alpha}4 Integrin-Dependent Leukocyte Recruitment Does Not Require VCAM-1 in a Chronic Model of Inflammation
J. Immunol.,
March 15, 2000;
164(6):
3337 - 3344.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Birner, T. B. Issekutz, U. Walter, and A. C. Issekutz
The role of {alpha}4 and LFA-1 integrins in selectin-independent monocyte and neutrophil migration to joints of rats with adjuvant arthritis
Int. Immunol.,
February 1, 2000;
12(2):
141 - 150.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ho and N. J. White
Molecular mechanisms of cytoadherence in malaria
Am J Physiol Cell Physiol,
June 1, 1999;
276(6):
C1231 - C1242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Walzog, K. Scharffetter-Kochanek, and P. Gaehtgens
Impairment of neutrophil emigration in CD18-null mice
Am J Physiol Gastrointest Liver Physiol,
May 1, 1999;
276(5):
G1125 - G1130.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Brodie, C. de la Rosa, J. G. Howe, J. Crouch, W. D. Travis, and K. Diem
Pediatric AIDS-Associated Lymphocytic Interstitial Pneumonia and Pulmonary Arterio-Occlusive Disease : Role of VCAM-1/VLA-4 Adhesion Pathway and Human Herpesviruses
Am. J. Pathol.,
May 1, 1999;
154(5):
1453 - 1464.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Catalina, P. Estess, and M. H. Siegelman
Selective Requirements for Leukocyte Adhesion Molecules in Models of Acute and Chronic Cutaneous Inflammation: Participation of E- and P- But Not L-Selectin
Blood,
January 15, 1999;
93(2):
580 - 589.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. H. Reinhardt and P. Kubes
Differential Leukocyte Recruitment From Whole Blood Via Endothelial Adhesion Molecules Under Shear Conditions
Blood,
December 15, 1998;
92(12):
4691 - 4699.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yamamoto, O. Kajikawa, T. R. Martin, S. R. Sharar, J. M. Harlan, and R. K. Winn2
The Role of Leukocyte Emigration and IL-8 on the Development of Lipopolysaccharide-Induced Lung Injury in Rabbits
J. Immunol.,
November 15, 1998;
161(10):
5704 - 5709.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. D. Patel
Eosinophil Tethering to Interleukin-4-Activated Endothelial Cells Requires Both P-Selectin and Vascular Cell Adhesion Molecule-1
Blood,
November 15, 1998;
92(10):
3904 - 3911.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Scalia, J. Z. Appel III, and A. M. Lefer
Leukocyte-Endothelium Interaction During the Early Stages of Hypercholesterolemia in the Rabbit : Role of P-Selectin, ICAM-1, and VCAM-1
Arterioscler. Thromb. Vasc. Biol.,
July 1, 1998;
18(7):
1093 - 1100.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Davenpeck, S. A. Sterbinsky, and B. S. Bochner
Rat Neutrophils Express alpha 4 and beta 1 Integrins and Bind to Vascular Cell Adhesion Molecule-1 (VCAM-1) and Mucosal Addressin Cell Adhesion Molecule-1 (MAdCAM-1)
Blood,
April 1, 1998;
91(7):
2341 - 2346.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
