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Clonal proliferation and cytokine requirement of murine progenitors for
natural killer cells
Y Aiba, F Hirayama and M Ogawa
Department of Medicine, Medical University of South Carolina, and the Ralph
H. Johnson Department of Veterans Affairs Medical Center, Charleston, USA.
We have established a clonal cell culture system that supports the
proliferation of committed natural killer (NK) cell progenitors of mice to
investigate the pathway and cytokine regulation of NK cell development. Day
14 fetal thymocytes cultured in methylcellulose with interleukin-7 (IL-7),
IL-15, and steel factor (SF) formed diffuse colonies that could not be
classified to known colony types. Single- cell origin of the colonies was
established by micromanipulation of the colony-forming cells. Cells in the
colonies are very blastic, showing no cytoplasmic differentiation, and
express Ly5, Thy-1, and CD25 but not myeloid, B, mature T, or NK cell
markers. The cells lack T, B, and myeloid potentials but can differentiate
to mature NK cells in fetal thymus organ culture, suggesting that the
colonies consist of NK committed progenitors. Examination of the minimal
cytokine requirement for the NK colony formation showed that IL-7 and SF
are indispensable for the formation of immature NK cell colonies. Both IL-2
and IL-15 increased the frequency of colonies. In contrast to IL-2, IL-7,
and IL- 15, IL-4 strongly inhibited the formation of the colonies. This
quantitative clonal culture will provide a useful means to examine the
mechanism of NK cell development.
Volume 89,
Issue 11,
pp. 4005-4012,
06/01/1997
Copyright © 1997 by The American Society of Hematology
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