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Potential and distribution of transplanted hematopoietic stem cells in a
nonablated mouse model
SK Nilsson, MS Dooner, CY Tiarks, HU Weier and PJ Quesenberry
Cancer Center, University of Massachusetts Medical Center, Worcester 01605,
USA.
Increasingly, allogeneic and even more often autologous bone marrow
transplants are being done to correct a wide variety of diseases. In
addition, autologous marrow transplants potentially provide an opportune
means of delivering genes in transfected, engrafting stem cells. However,
despite its widespread clinical use and promising gene therapy
applications, relatively little is known about the mechanisms of
engraftment in marrow transplant recipients. This is especially so in the
nonablated recipient setting. Our data show that purified lineage negative
rhodamine 123/Hoechst 33342 dull transplanted hematopoietic stem cells
engraft into the marrow of nonablated syngeneic recipients. These cells
have multilineage potential, and maintain a distinct subpopulation with
"stem cell" characteristics. The data also suggests a spatial localization
of stem cell "niches" to the endosteal surface, with all donor cells having
a high spatial affinity to this area. However, the level of stem cell
engraftment observed following a transplant of "stem cells" was
significantly lower than that expected following a transplant of the same
number of unseparated marrow cells from which the purified cells were
derived, suggesting the existence of a "nonstem cell facilitator
population," which is required in a nonablated syngeneic transplant
setting.
Volume 89,
Issue 11,
pp. 4013-4020,
06/01/1997
Copyright © 1997 by The American Society of Hematology
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