|
|
 Previous Article | Table of Contents | Next Article 
Kinetic evidence of the regeneration of multilineage hematopoiesis from
primitive cells in normal human bone marrow transplanted into
immunodeficient mice
JD Cashman, T Lapidot, JC Wang, M Doedens, LD Shultz, P Lansdorp, JE Dick and CJ Eaves
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, Canada.
Based on initial observations of human CD34+ Thy-1+ cells and long-term
culture-initiating cells (LTC-IC) in the bone marrow of some sublethally
irradiated severe combined immunodeficient (SCID) mice transplanted
intravenously with normal human marrow cells, and the subsequent finding
that the NOD/LtSz-scid/scid (NOD/SCID) mouse supports higher levels of
human cell engraftment, we undertook a series of time course experiments to
examine posttransplant changes in the number, tissue distribution, cycling
activity, and in vivo differentiation pattern of various human
hematopoietic progenitor cell populations in this latter mouse model. These
studies showed typical rapid posttransplant recovery curves for human CD34-
CD19+ (B-lineage) cells, CD34+ granulopoietic, erythroid, and multilineage
colony-forming cells (CFC), LTC-IC, and CD34+ Thy-1+ cells from a small
initial population representing <0.1% of the original transplant. The
most primitive human cell populations reached maximum values at 5 weeks
posttransplant, after which they declined. More mature cell types peaked
after another 5 weeks and then declined. A 2-week course of thrice weekly
injections of human Steel factor, interleukin (IL)-3,
granulocyte-macrophage colony-stimulating factor (GM-CSF), and
erythropoietin (administered just before the mice were killed for analysis)
did not alter the pace of regeneration of either primitive or mature human
hematopoietic cells, or their predominantly granulopoietic and B-lymphoid
pattern of differentiation, although a significant enhancing effect on the
level of human cell engraftment sustained after 3 months was noted. Cycling
studies showed the human CFC present at 4 to 5 weeks posttransplant to be
rapidly proliferating even in mice not given human growth factors. However,
by 10 weeks and thereafter, only quiescent human CFC were detected;
interestingly, even in mice that were given the 2-week course of growth
factor injections. These studies indicate the use of this model for future
analysis of the properties and in vivo regulation of primitive human
hematopoietic cells that possess in vivo repopulating ability.
Volume 89,
Issue 12,
pp. 4307-4316,
06/15/1997
Copyright © 1997 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
N. Matsuno, A. Wake, N. Uchida, K. Ishiwata, H. Araoka, S. Takagi, M. Tsuji, H. Yamamoto, D. Kato, Y. Matsuhashi, et al.
Impact of HLA disparity in the graft-versus-host direction on engraftment in adult patients receiving reduced-intensity cord blood transplantation
Blood,
August 20, 2009;
114(8):
1689 - 1695.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Yang, I. Dybedal, D. Bryder, L. Nilsson, E. Sitnicka, Y. Sasaki, and S. E. W. Jacobsen
IFN-{gamma} Negatively Modulates Self-Renewal of Repopulating Human Hemopoietic Stem Cells
J. Immunol.,
January 15, 2005;
174(2):
752 - 757.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Hess, T. E. Meyerrose, L. Wirthlin, T. P. Craft, P. E. Herrbrich, M. H. Creer, and J. A. Nolta
Functional characterization of highly purified human hematopoietic repopulating cells isolated according to aldehyde dehydrogenase activity
Blood,
September 15, 2004;
104(6):
1648 - 1655.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Thanopoulou, J. Cashman, T. Kakagianne, A. Eaves, N. Zoumbos, and C. Eaves
Engraftment of NOD/SCID-{beta}2 microglobulin null mice with multilineage neoplastic cells from patients with myelodysplastic syndrome
Blood,
June 1, 2004;
103(11):
4285 - 4293.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Mazurier, O. I. Gan, J. L. McKenzie, M. Doedens, and J. E. Dick
Lentivector-mediated clonal tracking reveals intrinsic heterogeneity in the human hematopoietic stem cell compartment and culture-induced stem cell impairment
Blood,
January 15, 2004;
103(2):
545 - 552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Segovia, G. Guenechea, J. M. Gallego, J. M. Almendral, and J. A. Bueren
Parvovirus Infection Suppresses Long-Term Repopulating Hematopoietic Stem Cells
J. Virol.,
August 1, 2003;
77(15):
8495 - 8503.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P. Kalberer, U. Siegler, and A. Wodnar-Filipowicz
Human NK cell development in NOD/SCID mice receiving grafts of cord blood CD34+ cells
Blood,
July 1, 2003;
102(1):
127 - 135.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yahata, K. Ando, T. Sato, H. Miyatake, Y. Nakamura, Y. Muguruma, S. Kato, and T. Hotta
A highly sensitive strategy for SCID-repopulating cell assay by direct injection of primitive human hematopoietic cells into NOD/SCID mice bone marrow
Blood,
April 15, 2003;
101(8):
2905 - 2913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Murdoch, K. Chadwick, M. Martin, F. Shojaei, K. V. Shah, L. Gallacher, R. T. Moon, and M. Bhatia
Wnt-5A augments repopulating capacity and primitive hematopoietic development of human blood stem cells invivo
PNAS,
March 18, 2003;
100(6):
3422 - 3427.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Laufs, B. Gentner, K. Z. Nagy, A. Jauch, A. Benner, S. Naundorf, K. Kuehlcke, B. Schiedlmeier, A. D. Ho, W. J. Zeller, et al.
Retroviral vector integration occurs in preferred genomic targets of human bone marrow-repopulating cells
Blood,
March 15, 2003;
101(6):
2191 - 2198.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Piacibello, S. Bruno, F. Sanavio, S. Droetto, M. Gunetti, L. Ailles, F. S. de Sio, A. Viale, L. Gammaitoni, A. Lombardo, et al.
Lentiviral gene transfer and ex vivo expansion of human primitive stem cells capable of primary, secondary, and tertiary multilineage repopulation in NOD/SCID mice
Blood,
December 15, 2002;
100(13):
4391 - 4400.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Chute, A. A. Saini, D. J. Chute, M. R. Wells, W. B. Clark, D. M. Harlan, J. Park, M. K. Stull, C. Civin, and T. A. Davis
Ex vivo culture with human brain endothelial cells increases the SCID-repopulating capacity of adult human bone marrow
Blood,
December 15, 2002;
100(13):
4433 - 4439.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cashman, B. Dykstra, I. Clark-Lewis, A. Eaves, and C. Eaves
Changes in the Proliferative Activity of Human Hematopoietic Stem Cells in NOD/SCID Mice and Enhancement of Their Transplantability after In Vivo Treatment with Cell Cycle Inhibitors
J. Exp. Med.,
November 4, 2002;
196(9):
1141 - 1150.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. E. Jay, L. Gallacher, and M. Bhatia
Emergence of muscle and neural hematopoiesis in humans
Blood,
October 16, 2002;
100(9):
3193 - 3202.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Saito, Y. Kametani, K. Hozumi, N. Mochida, K. Ando, M. Ito, T. Nomura, Y. Tokuda, H. Makuuchi, T. Tajima, et al.
The in vivo development of human T cells from CD34+ cells in the murine thymic environment
Int. Immunol.,
October 1, 2002;
14(10):
1113 - 1124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. C. Josephson, G. Vassilopoulos, G. D. Trobridge, G. V. Priestley, B. L. Wood, T. Papayannopoulou, and D. W. Russell
Transduction of human NOD/SCID-repopulating cells with both lymphoid and myeloid potential by foamy virus vectors
PNAS,
June 11, 2002;
99(12):
8295 - 8300.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cashman, I. Clark-Lewis, A. Eaves, and C. Eaves
Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice
Blood,
February 1, 2002;
99(3):
792 - 799.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. C. C. Kerre, G. De Smet, M. De Smedt, F. Offner, J. De Bosscher, J. Plum, and B. Vandekerckhove
Both CD34+38+ and CD34+38- Cells Home Specifically to the Bone Marrow of NOD/LtSZ scid/scid Mice but Show Different Kinetics in Expansion
J. Immunol.,
October 1, 2001;
167(7):
3692 - 3698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. I. D. Rossi, K. L. Medina, K. Garrett, G. Kolar, P. C. Comp, L. D. Shultz, J. D. Capra, P. Wilson, A. Schipul, and P. W. Kincade
Relatively Normal Human Lymphopoiesis but Rapid Turnover of Newly Formed B Cells in Transplanted Nonobese Diabetic/SCID Mice
J. Immunol.,
September 15, 2001;
167(6):
3033 - 3042.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Buske, M. Feuring-Buske, J. Antonchuk, P. Rosten, D. E. Hogge, C. J. Eaves, and R. K. Humphries
Overexpression of HOXA10 perturbs human lymphomyelopoiesis in vitro and in vivo
Blood,
April 15, 2001;
97(8):
2286 - 2292.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. E. Perez, H. M. Rinder, C. Wang, J. B. Tracey, N. Maun, and D. S. Krause
Xenotransplantation of immunodeficient mice with mobilized human blood CD34+ cells provides an in vivo model for human megakaryocytopoiesis and platelet production
Blood,
March 15, 2001;
97(6):
1635 - 1643.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. R. Appelbaum, J. M. Rowe, J. Radich, and J. E. Dick
Acute Myeloid Leukemia
Hematology,
January 1, 2001;
2001(1):
62 - 86.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Rosu-Myles, L. Gallacher, B. Murdoch, D. A. Hess, M. Keeney, D. Kelvin, L. Dale, S. S. G. Ferguson, D. Wu, F. Fellows, et al.
The human hematopoietic stem cell compartment is heterogeneous for CXCR4 expression
PNAS,
December 19, 2000;
97(26):
14626 - 14631.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Glimm, I.-H. Oh, and C. J. Eaves
Human hematopoietic stem cells stimulated to proliferate in vitro lose engraftment potential during their S/G2/M transit and do not reenter G0
Blood,
December 15, 2000;
96(13):
4185 - 4193.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-R. Goan, I. Junghahn, M. Wissler, M. Becker, J. Aumann, U. Just, G. Martiny-Baron, I. Fichtner, and R. Henschler
Donor stromal cells from human blood engraft in NOD/SCID mice
Blood,
December 1, 2000;
96(12):
3971 - 3978.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Cashman and C. J. Eaves
High marrow seeding efficiency of human lymphomyeloid repopulating cells in irradiated NOD/SCID mice
Blood,
December 1, 2000;
96(12):
3979 - 3981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Nilsson, I. Astrand-Grundstrom, I. Arvidsson, B. Jacobsson, E. Hellstrom-Lindberg, R. Hast, and S. E. W. Jacobsen
Isolation and characterization of hematopoietic progenitor/stem cells in 5q-deleted myelodysplastic syndromes: evidence for involvement at the hematopoietic stem cell level
Blood,
September 15, 2000;
96(6):
2012 - 2021.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wilpshaar, J. H. F. Falkenburg, X. Tong, W. A. Noort, R. Breese, D. Heilman, H. Kanhai, C. M. Orschell-Traycoff, and E. F. Srour
Similar repopulating capacity of mitotically active and resting umbilical cord blood CD34+ cells in NOD/SCID mice
Blood,
September 15, 2000;
96(6):
2100 - 2107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. F. Kelly, J. Vandergriff, A. Nathwani, A. W. Nienhuis, and E. F. Vanin
Highly efficient gene transfer into cord blood nonobese diabetic/severe combined immunodeficiency repopulating cells by oncoretroviral vector particles pseudotyped with the feline endogenous retrovirus (RD114) envelope protein
Blood,
August 15, 2000;
96(4):
1206 - 1214.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. W. Zandstra, D. A. Lauffenburger, and C. J. Eaves
A ligand-receptor signaling threshold model of stem cell differentiation control: a biologically conserved mechanism applicable to hematopoiesis
Blood,
August 15, 2000;
96(4):
1215 - 1222.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. F. Srour;, C. Eaves, and H. Glimm
Proliferative history and hematopoietic function of ex vivo expanded human CD34+ cells
Blood,
August 15, 2000;
96(4):
1609 - 1612.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Peled, O. Kollet, T. Ponomaryov, I. Petit, S. Franitza, V. Grabovsky, M. M. Slav, A. Nagler, O. Lider, R. Alon, et al.
The chemokine SDF-1 activates the integrins LFA-1, VLA-4, and VLA-5 on immature human CD34+ cells: role in transendothelial/stromal migration and engraftment of NOD/SCID mice
Blood,
June 1, 2000;
95(11):
3289 - 3296.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Kollet, A. Peled, T. Byk, H. Ben-Hur, D. Greiner, L. Shultz, and T. Lapidot
beta 2 Microglobulin-deficient (B2mnull) NOD/SCID mice are excellent recipients for studying human stem cell function
Blood,
May 15, 2000;
95(10):
3102 - 3105.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. D. Shultz, P. A. Lang, S. W. Christianson, B. Gott, B. Lyons, S. Umeda, E. Leiter, R. Hesselton, E. J. Wagar, J. H. Leif, et al.
NOD/LtSz-Rag1null Mice: An Immunodeficient and Radioresistant Model for Engraftment of Human Hematolymphoid Cells, HIV Infection, and Adoptive Transfer of NOD Mouse Diabetogenic T Cells
J. Immunol.,
March 1, 2000;
164(5):
2496 - 2507.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Schiedlmeier, K. Kuhlcke, H. G. Eckert, C. Baum, W. J. Zeller, and S. Fruehauf
Quantitative assessment of retroviral transfer of the human multidrug resistance 1 gene to human mobilized peripheral blood progenitor cells engrafted in nonobese diabetic/severe combined immunodeficient mice
Blood,
February 15, 2000;
95(4):
1237 - 1248.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Cobaleda, N. Gutierrez-Cianca, J. Perez-Losada, T. Flores, R. Garcia-Sanz, M. Gonzalez, and I. Sanchez-Garcia
A primitive hematopoietic cell is the target for the leukemic transformation in human Philadelphia-positive acute lymphoblastic leukemia
Blood,
February 1, 2000;
95(3):
1007 - 1013.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.D. Cashman, I. Clark-Lewis, A.C. Eaves, and C.J. Eaves
Differentiation Stage-Specific Regulation of Primitive Human Hematopoietic Progenitor Cycling by Exogenous and Endogenous Inhibitors in an In Vivo Model
Blood,
December 1, 1999;
94(11):
3722 - 3729.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. E. Nicolini, T. L. Holyoake, J. D. Cashman, P. P.Y. Chu, K. Lambie, and C. J. Eaves
Unique Differentiation Programs of Human Fetal Liver Stem Cells Shown Both In Vitro and In Vivo in NOD/SCID Mice
Blood,
October 15, 1999;
94(8):
2686 - 2695.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Glimm and C.J. Eaves
Direct Evidence for Multiple Self-Renewal Divisions of Human In Vivo Repopulating Hematopoietic Cells in Short-Term Culture
Blood,
October 1, 1999;
94(7):
2161 - 2168.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. D. Zanjani, A. W. Flake, G. Almeida-Porada, N. Tran, and T. Papayannopoulou
Homing of Human Cells in the Fetal Sheep Model: Modulation by Antibodies Activating or Inhibiting Very Late Activation Antigen-4-Dependent Function
Blood,
October 1, 1999;
94(7):
2515 - 2522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. E. Ailles, B. Gerhard, H. Kawagoe, and D. E. Hogge
Growth Characteristics of Acute Myelogenous Leukemia Progenitors That Initiate Malignant Hematopoiesis in Nonobese Diabetic/Severe Combined Immunodeficient Mice
Blood,
September 1, 1999;
94(5):
1761 - 1772.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. S. Gerhard
Inter-Strain Variation in Murine Models of Leukemia
Blood,
August 1, 1999;
94(3):
1142 - 1142.
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Piacibello, F. Sanavio, A. Severino, A. Dane, L. Gammaitoni, F. Fagioli, E. Perissinotto, G. Cavalloni, O. Kollet, T. Lapidot, et al.
Engraftment in Nonobese Diabetic Severe Combined Immunodeficient Mice of Human CD34+ Cord Blood Cells After Ex Vivo Expansion: Evidence for the Amplification and Self-Renewal of Repopulating Stem Cells
Blood,
June 1, 1999;
93(11):
3736 - 3749.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. I. Rebel, M. Tanaka, J.-S. Lee, S. Hartnett, M. Pulsipher, D. G. Nathan, R. C. Mulligan, and C. A. Sieff
One-Day Ex Vivo Culture Allows Effective Gene Transfer Into Human Nonobese Diabetic/Severe Combined Immune-Deficient Repopulating Cells Using High-Titer Vesicular Stomatitis Virus G Protein Pseudotyped Retrovirus
Blood,
April 1, 1999;
93(7):
2217 - 2224.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Peled, I. Petit, O. Kollet, M. Magid, T. Ponomaryov, T. Byk, A. Nagler, H. Ben-Hur, A. Many, L. Shultz, et al.
Dependence of Human Stem Cell Engraftment and Repopulation of NOD/SCID Mice on CXCR4
Science,
February 5, 1999;
283(5403):
845 - 848.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. D. Cashman and C. J. Eaves
Human Growth Factor-Enhanced Regeneration of Transplantable Human Hematopoietic Stem Cells in Nonobese Diabetic/Severe Combined Immunodeficient Mice
Blood,
January 15, 1999;
93(2):
481 - 487.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Schilz, G. Brouns, O. G. Ottmann, D. Hoelzer, A. A. Fauser, A. J. Thrasher, and M. Grez
High Efficiency Gene Transfer to Human Hematopoietic SCID-Repopulating Cells Under Serum-Free Conditions
Blood,
November 1, 1998;
92(9):
3163 - 3171.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Gothot, J. C.M. van der Loo, D. W. Clapp, and E. F. Srour
Cell Cycle-Related Changes in Repopulating Capacity of Human Mobilized Peripheral Blood CD34+ Cells in Non-Obese Diabetic/Severe Combined Immune-Deficient Mice
Blood,
October 15, 1998;
92(8):
2641 - 2649.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C.M. van der Loo, H. Hanenberg, R. J. Cooper, F.-Y. Luo, E. N. Lazaridis, and D. A. Williams
Nonobese Diabetic/Severe Combined Immunodeficiency (NOD/SCID) Mouse as a Model System to Study the Engraftment and Mobilization of Human Peripheral Blood Stem Cells
Blood,
October 1, 1998;
92(7):
2556 - 2570.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.C.Y. Wang, T. Lapidot, J.D. Cashman, M. Doedens, L. Addy, D.R. Sutherland, R. Nayar, P. Laraya, M. Minden, A. Keating, et al.
High Level Engraftment of NOD/SCID Mice by Primitive Normal and Leukemic Hematopoietic Cells From Patients With Chronic Myeloid Leukemia in Chronic Phase
Blood,
April 1, 1998;
91(7):
2406 - 2414.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M.A. Verstegen, P. B. van Hennik, W. Terpstra, C. van den Bos, J. J. Wielenga, N. van Rooijen, R. E. Ploemacher, G. Wagemaker, and A. W. Wognum
Transplantation of Human Umbilical Cord Blood Cells in Macrophage-Depleted SCID Mice: Evidence for Accessory Cell Involvement in Expansion of Immature CD34+CD38- Cells
Blood,
March 15, 1998;
91(6):
1966 - 1976.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. D. Lewis, L. A. McDiarmid, L. M. Samels, L. Bik To, and T. P. Hughes
Establishment of a Reproducible Model of Chronic-Phase Chronic Myeloid Leukemia in NOD/SCID Mice Using Blood-Derived Mononuclear or CD34+ Cells
Blood,
January 15, 1998;
91(2):
630 - 640.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Conneally, J. Cashman, A. Petzer, and C. Eaves
Expansion in vitro of transplantable human cord blood stem cells demonstrated using a quantitative assay of their lympho-myeloid repopulating activity in nonobese diabetic-scid/scid mice
PNAS,
September 2, 1997;
94(18):
9836 - 9841.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
