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Interleukin-12 (IL-12) production in whole blood cultures from human
immunodeficiency virus-infected individuals studied in relation to IL- 10
and prostaglandin E2 production
L Meyaard, E Hovenkamp, N Pakker, TC van der Pouw Kraan and F Miedema
Department of Clinical Viro-Immunology, Central Laboratory of the
Netherlands Red Cross Blood Transfusion Service, Amsterdam, The
Netherlands.
The role of interleukin-12 (IL-12) in Th1 cell differentiation is well
established. The heterodimer p70, composed of a p40 and a p35 chain, is the
biologically active form. IL-12 production by human monocytes is enhanced
by interferon-gamma (IFN-gamma) and inhibited by IL-10 and prostaglandin E2
(PGE2). Peripheral blood mononuclear cells from human immunodeficiency
virus (HIV)-infected individuals reportedly have impaired IL-12 p40 and p70
production on stimulation with Staphylococcus aureus Cowan I (SAC) in
vitro. Both PGE2 and IL-10 previously were proposed to be instrumental in
this defect in IL-12 production. Here, we studied IL-12 p40 and p70
production in relation to IL-10 and PGE2 production in whole blood cultures
from HIV-infected individuals. On stimulation with lipopolysaccharide,
IL-12 production was normal. However, on stimulation with SAC, IL-12 p40
and p70 production was decreased in HIV-infected individuals and correlated
significantly with decreased peripheral blood CD4+ T-cell number and T-
cell reactivity to CD3 monoclonal antibody in vitro. However, IL-10 and
PGE2 production in cultures from HIV-infected individuals was normal and
did not relate to IL-12 production. In conclusion, IL-12 production by
cells from HIV-infected individuals is impaired under certain conditions in
vitro and this decrease is independent of IL-10 or PGE2 production.
Volume 89,
Issue 2,
pp. 570-576,
01/15/1997
Copyright © 1997 by The American Society of Hematology
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