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Population size and radiosensitivity of murine hematopoietic endogenous
long-term repopulating cells
KF McCarthy
A new assay is described that measures the numbers (n) of endogenous
long-term repopulating cells (eLTRC) surviving sublethal irradiation. The
assay is based on analysis of variances of Pgk-1 phenotypes within groups
of sublethally irradiated Pgk-1(a/b) mice. When ln n is plotted as a
function of dose, the radiosensitivity (D0) is the negative reciprocal of
the slope and the gamma-intercept is N or total numbers of eLTRC per mouse.
The assay is unique in that N is an absolute value not requiring correction
for seeding efficiency, or f. From two independent estimates, total numbers
of eLTRC were determined to be 8,700 or 11,900 cells per mouse and D0 was
found to be 0.82 or 0.83 Gy. If eLTRC are, in fact, the long-term
repopulating cells (LTRC) defined by classical transfer assay, then LTRC
can home to the marrow with nearly unit efficiency, only one to two LTRC
are required for a mouse to survive a radiation LD50/30, and LTRC possess
nearly unlimited self- renewal potential.
Volume 89,
Issue 3,
pp. 834-841,
02/01/1997
Copyright © 1997 by The American Society of Hematology
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