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Initiation of murine embryonic erythropoiesis: a spatial analysis
L Silver and J Palis
University of Rochester Medical Center, Department of Pediatrics and Cancer
Center, NY 14642, USA.
Hematopoiesis in the mouse conceptus begins in the visceral yolk (VYS),
with primitive erythroblasts first evident in blood islands at the headfold
stage (E8.0). VYS erythropoiesis is decreased or abrogated by targeted
disruption of the hematopoietic transcription factors tal-1, rbtn2, GATA-1,
and GATA-2. To better understand the potential roles of these genes, and to
trace the initial temporal and spatial development of mammalian embryonic
hematopoiesis, we examined their expression patterns, and that of
betaH1-globin, in normal mouse conceptuses by means of in situ
hybridization. Attention was focused on the 36-hour period from
mid-primitive streak to early somite stages (E7.25 to E8.5), when the
conceptus undergoes rapid morphologic changes with formation of the yolk
sac and blood islands. Each of these genes was expressed in extraembryonic
mesoderm, from which blood islands are derived. This VYS expression
occurred in a defined temporal sequence: tal-1 and rbtn2 transcripts were
detected earlier than the others, followed by GATA-2 and GATA-1, and then
by betaH1-globin. Transcripts for all of these genes were present in VYS
mesoderm cell masses at the neural plate stage (E7.5), indicating
commitment of these cells to the erythroid lineage before the appearance of
morphologically recognizable erythroblasts. By early somite stages (E8.5),
GATA-2 mRNA expression is downregulated in VYS blood islands as terminal
primitive erythroid differentiation proceeds. We conclude that primitive
mammalian erythropoiesis arises during gastrulation through the ordered
temporal expression of tal-1, rbtn2, GATA2, and GATA-1 in a subset of
extraembryonic mesoderm cells. During the stages analyzed, tal-1 and rbtn2
expression was also present in posterior embryonic mesoderm, while GATA-1
and GATA-2 expression was evident in extraembryonic tissues of ectodermal
origin.
Volume 89,
Issue 4,
pp. 1154-1164,
02/15/1997
Copyright © 1997 by The American Society of Hematology
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