|
|
 Previous Article | Table of Contents | Next Article 
Cell-cycle-dependent regulation of erythropoietin receptor gene
N Komatsu, K Kirito, Y Kashii, Y Furukawa, J Kikuchi, N Suwabe, M Yamamoto and Y Miura
Department of Medicine, Institute of Hematology, Jichi Medical School,
Tochigi-ken, Japan.
To understand the regulatory mechanism of erythropoietin (EPO) receptor
(EPOR) gene expression, the effect of EPO on the steady-state level of EPOR
mRNA was examined using the human EPO-dependent cell line UT-7 as a model
system. We found that the treatment of UT-7 cells with EPO resulted in a
transient decrease of the EPOR mRNA level. This transient downregulation
was also induced by stimulation with granulocyte- macrophage
colony-stimulating factor (GM-CSF), another stimulator of UT- 7 cell
growth. These results raised the possibility that EPOR gene expression is
in part related to cell growth. Moreover, it was found that EPO-induced
downregulation of EPOR mRNA level was preceded by a transient
downregulation of GATA-1 mRNA. To examine the relationship between the
expression of EPOR, GATA-1, and GATA-2 mRNA levels and the cell cycle,
logarithmically growing UT-7 cells were centrifugically fractionated
according to the cell-cycle phase. Both EPOR and GATA-1 mRNA levels, but
not the GATA-2 mRNA level, concomitantly decreased at the G0/G1 phase and
increased at the S and G2/M phases. An electrophoretic mobility shift assay
(EMSA) showed that in EPO- stimulated UT-7 cells, the dynamic changes in
EPOR gene expression paralleled the GATA-1 DNA-binding activity to the
oligonucleotide probe containing a GATA-binding site located at the
promoter region of the EPOR gene. These findings suggest that the
regulation of EPOR mRNA level is mainly associated with GATA-1 gene
expression in UT-7 cells undergoing proliferation, and that these serial
events are under the control of, or related to, the cell cycle.
Volume 89,
Issue 4,
pp. 1182-1188,
02/15/1997
Copyright © 1997 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K. M. Kirschner, P. Hagen, C. S. Hussels, M. Ballmaier, H. Scholz, and C. Dame
The Wilms' tumor suppressor Wt1 activates transcription of the erythropoietin receptor in hematopoietic progenitor cells
FASEB J,
August 1, 2008;
22(8):
2690 - 2701.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. R. LaMontagne, J. Butler, D. J. Marshall, J. Tullai, Z. Gechtman, C. Hall, A. Meshaw, and F. X. Farrell
Recombinant epoetins do not stimulate tumor growth in erythropoietin receptor-positive breast carcinoma models.
Mol. Cancer Ther.,
February 1, 2006;
5(2):
347 - 355.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Verdier, P. Walrafen, N. Hubert, S. Chretien, S. Gisselbrecht, C. Lacombe, and P. Mayeux
Proteasomes Regulate the Duration of Erythropoietin Receptor Activation by Controlling Down-regulation of Cell Surface Receptors
J. Biol. Chem.,
June 9, 2000;
275(24):
18375 - 18381.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
