|
|
 Previous Article | Table of Contents | Next Article 
Thrombopoietin enhances proliferation and differentiation of murine yolk
sac erythroid progenitors
T Era, T Takahashi, K Sakai, K Kawamura and T Nakano
Department of Molecular Cell Biology, Research Institute for Microbial
Diseases, Osaka University, Suita, Japan.
Thrombopoietin (TPO), the ligand for the receptor proto-oncogene c-Mpl, has
been cloned and shown to be the critical regulator of proliferation and
differentiation of megakaryocytic lineage. Initially, TPO was not
considered to have the activity on hematopoietic lineages other than
megakaryocytes. Recently, however, TPO was reported to enhance the in vitro
erythroid colony formation from human bone marrow (BM) CD34+ progenitors or
from mouse BM cells in combination with other cytokines. We examined the
effects of TPO on the colony formation of hematopoietic progenitors in
mouse yolk sac. TPO remarkably enhanced proliferation and differentiation
of erythroid-lineage cells in the presence of erythropoietin (Epo). This
effect was observed even in the absence of Epo. Compared with adult BM,
yolk sac turned out to have relatively abundant erythroid and
erythro-megakaryocytic progenitors, which responded to TPO and Epo
stimulation. TPO similarly stimulated erythroid colony formation from in
vitro differentiation-induced mouse embryonic stem (ES) cells whose
hematopoietic differentiation status was similar to that of yolk sac. These
findings help to understand the biology of hematopoietic progenitors of the
early phase of hematopoiesis. Yolk sac cells or in vitro
differentiation-induced ES cells would be good sources to analyze the TPO
function on erythropoiesis.
Volume 89,
Issue 4,
pp. 1207-1213,
02/15/1997
Copyright © 1997 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K. Kitajima, J. Zheng, H. Yen, D. Sugiyama, and T. Nakano
Multipotential differentiation ability of GATA-1-null erythroid-committed cells.
Genes & Dev.,
March 15, 2006;
20(6):
654 - 659.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Xie, R. J. Chan, S. A. Johnson, M. Starr, J. McCarthy, R. Kapur, and M. C. Yoder
Thrombopoietin promotes mixed lineage and megakaryocytic colony-forming cell growth but inhibits primitive and definitive erythropoiesis in cells isolated from early murine yolk sacs
Blood,
February 15, 2003;
101(4):
1329 - 1335.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ando, Y. Iwamoto, A. Suda, K. Tsuchiya, and H. Nihei
New insights into the thrombopoietic status of patients on dialysis through the evaluation of megakaryocytopoiesis in bone marrow and of endogenous thrombopoietin levels
Blood,
February 15, 2001;
97(4):
915 - 921.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-S. Luo, K. Ogata, N. Yokose, T. Kato, and K. Dan
Effect of Thrombopoietin on Proliferation of Blasts from Patients with Myelodysplastic Syndromes
Stem Cells,
March 1, 2000;
18(2):
112 - 119.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Era and O. N. Witte
Regulated expression of P210 Bcr-Abl during embryonic stem cell differentiation stimulates multipotential progenitor expansion and myeloid cell fate
PNAS,
February 15, 2000;
97(4):
1737 - 1742.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Era, T. Takagi, T. Takahashi, J.-C. Bories, and T. Nakano
Characterization of hematopoietic lineage-specific gene expression by ES cell in vitro differentiation induction system
Blood,
February 1, 2000;
95(3):
870 - 878.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Crapnell, E. D. Zanjani, A. Chaudhuri, J. L. Ascensao, S. St. Jeor, and J. P. Maciejewski
In vitro infection of megakaryocytes and their precursors by human cytomegalovirus
Blood,
January 15, 2000;
95(2):
487 - 493.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Motoyama, T. Kimura, T. Takahashi, T. Watanabe, and T. Nakano
bcl-x Prevents Apoptotic Cell Death of Both Primitive and Definitive Erythrocytes at the End of Maturation
J. Exp. Med.,
June 7, 1999;
189(11):
1691 - 1698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kato, A. Matsumoto, K. Ogami, T. Tahara, H. Morita, and H. Miyazaki
Native Thrombopoietin: Structure and Function
Stem Cells,
September 1, 1998;
16(5):
322 - 328.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. C. Gregory, N. Jiang, K. Todokoro, J. Crouse, R. E. Pacifici, and D. M. Wojchowski
Erythropoietin Receptor and STAT5-Specific Pathways Promote SKT6 Cell Hemoglobinization
Blood,
August 15, 1998;
92(4):
1104 - 1118.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Guerriero, L. Worford, H. K. Holland, G.-R. Guo, K. Sheehan, and E. K. Waller
Thrombopoietin Is Synthesized by Bone Marrow Stromal Cells
Blood,
November 1, 1997;
90(9):
3444 - 3455.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
