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Evidence for a large compartment of IgM-expressing memory B cells in humans
U Klein, R Kuppers and K Rajewsky
Institute for Genetics, University of Cologne, Germany.
The recent finding of somatically mutated mu heavy chain transcripts in
human peripheral blood (PB) B lymphocytes suggests that T-dependent B- cell
memory might not be restricted to class-switched cells. We provide here
evidence that IgM-only PB B cells are likely to be the IgM- expressing
counterpart of classical (IgM- IgD-) memory B cells in humans. As shown by
molecular single cell analysis, most IgM-only cells carry mutated V region
genes, like class-switched cells. Although both subsets represent
populations of nonactivated, resting cells, they express higher levels of
Ig mRNA than naive (IgM+ IgD+) B cells. IgM- only and class-switched cells
are CD38- CD77-, and mostly CD23-, thus neither resembling germinal center
nor naive B cells. Because many IgM- expressing B cells located in
secondary lymphoid tissues resemble IgM- only PB B cells in terms of cell
phenotype, we propose that the human lymphoid system contains a large
compartment of IgM-expressing memory cells. Moreover, these cells seem to
represent the nonmalignant counterparts of IgM-expressing tumor cells in
sporadic Burkitt's lymphoma, MALT lymphoma, monocytoid B-cell lymphoma, and
diffuse large- cell lymphoma that were found to harbor somatically mutated
V genes.
Volume 89,
Issue 4,
pp. 1288-1298,
02/15/1997
Copyright © 1997 by The American Society of Hematology
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