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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Broudy, V. C. Articles by Kaushansky, K. Search for Related Content PubMed PubMed Citation Articles by Broudy, V. C. Articles by Kaushansky, K. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Human platelets display high-affinity receptors for thrombopoietin VC Broudy, NL Lin, DF Sabath, T Papayannopoulou and K Kaushansky Department of Medicine, University of Washington, Seattle 98195, USA. Thrombopoietin (Tpo) is a major regulator of megakaryopoiesis both in vivo and in vitro. Tpo initiates its biologic effects by binding to the Mpl receptor, which is a member of the hematopoietin receptor family. To define the Tpo binding characteristics of the Mpl receptor, we iodinated purified 70-kD recombinant human Tpo using the Bolton-Hunter reagent. Autoradiographic analysis of (125)I-Tpo binding to normal human marrow mononuclear cells showed many grains specifically associated with megakaryocytes; there were no grains specifically associated with myeloblasts or erythroblasts. Equilibrium binding experiments with (125)I-Tpo and normal human platelets showed a single class of high-affinity receptors (kd, 190 pmol/L) with approximately 30 Mpl receptors per platelet. Affinity cross-linking with (125)I-Tpo showed that the Mpl receptor on platelets is of molecular weight approximately 98 kD. Despite their sequence similarity, erythropoietin and Tpo did not cross-compete for binding to BaF3 cells engineered to coexpress Mpl receptor and erythropoietin receptor. Progeny of normal human burst-forming units-erythroid (BFU-E) contained Mpl receptor mRNA, and flow cytometric analysis showed the presence of Mpl receptor protein on the surface of these cells. These data indicate that display of the Mpl receptor is not limited to the megakaryocytic lineage, but also includes progeny of BFU-E. Like receptors for other hematopoietic cytokines, the binding affinity of the Mpl receptor for Tpo is high, with relatively few receptors displayed per cell. These results suggest that the effects of Tpo to speed red blood cell recovery after myelosuppressive therapy in vivo and to enhance colony-forming unit- erythroid generation in vitro may be mediated by direct interaction of Tpo and erythroid progenitor cells. Volume 89, Issue 6, pp. 1896-1904, 03/15/1997 Copyright © 1997 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. J. Kuter New thrombopoietic growth factors Blood, June 1, 2007; 109(11): 4607 - 4616. [Abstract] [Full Text] [PDF] Y. Royer, J. Staerk, M. Costuleanu, P. J. Courtoy, and S. N. Constantinescu Janus Kinases Affect Thrombopoietin Receptor Cell Surface Localization and Stability J. Biol. Chem., July 22, 2005; 280(29): 27251 - 27261. [Abstract] [Full Text] [PDF] H. Ehrenreich, M. Hasselblatt, F. Knerlich, N. von Ahsen, S. Jacob, S. Sperling, H. Woldt, K. Vehmeyer, K.-A. Nave, and A.-L. Siren A hematopoietic growth factor, thrombopoietin, has a proapoptotic role in the brain PNAS, January 18, 2005; 102(3): 862 - 867. [Abstract] [Full Text] [PDF] D. D. Dahlen, V. C. Broudy, and J. G. 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	Copyright © 1997 by American Society of Hematology         Online ISSN: 1528-0020