|
|
 Previous Article | Table of Contents | Next Article 
CD44-mediated adhesiveness of human hematopoietic progenitors to hyaluronan
is modulated by cytokines
S Legras, JP Levesque, R Charrad, K Morimoto, C Le Bousse, D Clay, C Jasmin and F Smadja-Joffe
Institut National de la Sante et de la Recherche Medicale U268, Hopital
Paul Brousse, Villejuif, France.
Adhesive interactions between CD34+ hematopoietic progenitor cells (HPC)
and bone marrow stroma are crucial for normal hematopoiesis, yet their
molecular bases are still poorly elucidated. We have investigated whether
cell surface proteoglycan CD44 can mediate adhesion of human CD34+ HPC to
immobilized hyaluronan (HA), an abundant glycosaminoglycan of the bone
marrow extracellular matrix. Our data show that, although CD34+ cells
strongly express CD44, only 13.3% +/- 1.1% spontaneously adheres to HA.
Short-term methylcellulose assay showed that HA-adherent CD34+ cells
comprised granulo-monocytic and erythroid committed progenitors (19.6% +/-
2.5% and 7.3% +/- 1.0% of the input, respectively). More primitive
progenitors, such as pre-colony-forming units, also adhered to HA.
Moreover, we found that CD44-mediated adhesion of CD34+ cells to HA could
be enhanced by phorbol 12-myristate 13-acetate (PMA), the
function-activating anti-CD44 monoclonal antibody H90, and cytokines such
as granulocyte-monocyte colony-stimulating factor, interleukin-3 (IL-3),
and stem cell factor. Enhancement through PMA required several hours, was
protein-synthesis-dependent, and was associated with an increase of CD44
cell surface expression, whereas stimulation of adhesion by H90 monoclonal
antibody and cytokines was very rapid and without alteration of CD44
expression. H90-induced activation occurred at 4 degrees C and lasted for
at least 2 hours, whereas activation by cytokines required incubation at 37
degrees C and was transient. These data, which show for the first time that
CD34+ HPC can directly adhere to HA via CD44, point out that this adhesive
interaction to HA is a process that may also be physiologically regulated
by cytokines.
Volume 89,
Issue 6,
pp. 1905-1914,
03/15/1997
Copyright © 1997 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. MENDEZ-FERRER and P. S. FRENETTE
Hematopoietic Stem Cell Trafficking: Regulated Adhesion and Attraction to Bone Marrow Microenvironment
Ann. N.Y. Acad. Sci.,
November 1, 2007;
1116(1):
392 - 413.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Mishra, S. Mishra, K. Gee, and A. Kumar
Differential Involvement of Calmodulin-dependent Protein Kinase II-activated AP-1 and c-Jun N-terminal Kinase-activated EGR-1 Signaling Pathways in Tumor Necrosis Factor-{alpha} and Lipopolysaccharide-induced CD44 Expression in Human Monocytic Cells
J. Biol. Chem.,
July 22, 2005;
280(29):
26825 - 26837.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Gadhoum, M.-P. Leibovitch, J. Qi, D. Dumenil, L. Durand, S. Leibovitch, and F. Smadja-Joffe
CD44: a new means to inhibit acute myeloid leukemia cell proliferation via p27Kip1
Blood,
February 1, 2004;
103(3):
1059 - 1068.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Gee, W. Lim, W. Ma, D. Nandan, F. Diaz-Mitoma, M. Kozlowski, and A. Kumar
Differential Regulation of CD44 Expression by Lipopolysaccharide (LPS) and TNF-{alpha} in Human Monocytic Cells: Distinct Involvement of c-Jun N-Terminal Kinase in LPS-Induced CD44 Expression
J. Immunol.,
November 15, 2002;
169(10):
5660 - 5672.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Delcommenne, R. Kannagi, and P. Johnson
TNF-{alpha} increases the carbohydrate sulfation of CD44: induction of 6-sulfo N-acetyl lactosamine on N- and O-linked glycans
Glycobiology,
October 1, 2002;
12(10):
613 - 622.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Termeer, H. Johannsen, T. Braun, A. Renkl, T. Ahrens, R. W. Denfeld, M. B. Lappin, J. M. Weiss, and J. C. Simon
The role of CD44 during CD40 ligand-induced dendritic cell clustering and maturation
J. Leukoc. Biol.,
November 1, 2001;
70(5):
715 - 722.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Lee, S.-A. Im, E.-S. Yoo, E.-M. Nam, M.-A. Lee, J.-Y. Ahn, J.-W. Huh, D.-Y. Kim, S.-N. Lee, M.-J. Kim, et al.
Mobilization Kinetics of CD34+ Cells in Association with Modulation of CD44 and CD31 Expression during Continuous Intravenous Administration of G-CSF in Normal Donors
Stem Cells,
July 1, 2000;
18(4):
281 - 286.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
L. M. Pilarski, E. Pruski, J. Wizniak, D. Paine, K. Seeberger, M. J. Mant, C. B. Brown, and A. R. Belch
Potential Role for Hyaluronan and the Hyaluronan Receptor RHAMM in Mobilization and Trafficking of Hematopoietic Progenitor Cells
Blood,
May 1, 1999;
93(9):
2918 - 2927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Fitzgerald and L. A. J. O'Neill
Characterization of CD44 Induction by IL-1: A Critical Role for Egr-1
J. Immunol.,
April 15, 1999;
162(8):
4920 - 4927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Gothot, J. C.M. van der Loo, D. W. Clapp, and E. F. Srour
Cell Cycle-Related Changes in Repopulating Capacity of Human Mobilized Peripheral Blood CD34+ Cells in Non-Obese Diabetic/Severe Combined Immune-Deficient Mice
Blood,
October 15, 1998;
92(8):
2641 - 2649.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Vermeulen, F. Le Pesteur, M.-C. Gagnerault, J.-Y. Mary, F. Sainteny, and F. Lepault
Role of Adhesion Molecules in the Homing and Mobilization of Murine Hematopoietic Stem and Progenitor Cells
Blood,
August 1, 1998;
92(3):
894 - 900.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Legras, U. Gunthert, R. Stauder, F. Curt, S. Oliferenko, H.C. Kluin-Nelemans, J.P. Marie, S. Proctor, C. Jasmin, and F. Smadja-Joffe
A Strong Expression of CD44-6v Correlates With Shorter Survival of Patients With Acute Myeloid Leukemia
Blood,
May 1, 1998;
91(9):
3401 - 3413.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. P. Skelton, C. Zeng, A. Nocks, and I. Stamenkovic
Glycosylation Provides Both Stimulatory and Inhibitory Effects on Cell Surface and Soluble CD44 Binding to Hyaluronan
J. Cell Biol.,
January 26, 1998;
140(2):
431 - 446.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
