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Granulocyte colony-stimulating factor-induced comobilization of CD4- CD8- T
cells and hematopoietic progenitor cells (CD34+) in the blood of normal
donors
CR Kusnierz-Glaz, BJ Still, M Amano, JD Zukor, RS Negrin, KG Blume and S Strober
Department of Medicine, Stanford University School of Medicine, CA
94305-5111, USA.
The feasibility of transplantation of HLA-matched hematopoietic progenitor
cells from the blood of normal donors given granulocyte colony-stimulating
factor (G-CSF) has been reported recently. In the current study, the
changes in T-cell subsets as well as CD34+ cells were determined in one
blood volume leukapheresis products of six normal individuals given G-CSF.
Examination of the T-cell subsets in the leukapheresis products showed
three different patterns: one in which a discrete population of CD4- CD8-
alphabeta T cells was found in addition to the typical CD4+ and CD8+ T
cells in the unfractionated as well as in high- and low-density cells; a
second in which the discrete population of CD4- CD8- alphabeta T cells was
predominant only in the low-density fractions; and a third in which a
discrete population of CD4- CD8- T cells was not observed. The median yield
of CD4- CD8- T cells was about fourfold to fivefold higher than the
calculated number present in one blood volume (5L) from normal individuals.
The ratios of CD34+ cells to CD4+ and CD8+ T cells, and of CD4- CD8- T
cells to CD4+ and CD8+ T cells, were highest in the low-density fractions.
These fractions suppressed the mixed leukocyte, and may ameliorate graft-
versus-host disease as compared with unfractionated cells.
Volume 89,
Issue 7,
pp. 2586-2595,
04/01/1997
Copyright © 1997 by The American Society of Hematology
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