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Selective expression of the receptor tyrosine kinase, HTK, on human
erythroid progenitor cells
T Inada, A Iwama, S Sakano, M Ohno, K Sawada and T Suda
Department of Cell Differentiation, Kumamoto University School of Medicine,
Kumamoto City, Japan.
HTK is a receptor tyrosine kinase of the Eph family. To characterize the
involvement of HTK in hematopoiesis, we generated monoclonal antibodies
against HTK and investigated its expression on human bone marrow cells.
About 5% of the bone marrow cells were HTK+, which were also c-Kit+,
CD34(low), and glycophorin A(-/low). Assays of progenitors showed that HTK+
c-Kit+ cells consisted exclusively of erythroid progenitors, whereas HTK-
c-Kit+ cells contained progenitors of granulocytes and macrophages as well
as those of erythroid cells. Most of the HTK+ erythroid progenitors were
stem cell factor-dependent for proliferation, indicating that they
represent mainly erythroid burst- forming units (BFU-E). During the
erythroid differentiation of cultured peripheral CD34+ cells, HTK
expression was upregulated on immature erythroid cells that corresponded to
BFU-E and erythroid colony-forming units and downregulated on erythroblasts
with high levels of glycophorin expression. These findings suggest that HTK
is selectively expressed on the restricted stage of erythroid progenitors,
particularly BFU-E, and that HTK is the first marker antigen that allows
the purification of erythroid progenitors. Furthermore, HTKL, the ligand
for HTK, was expressed in the bone marrow stromal cells. Our findings
provide a novel regulatory system of erythropoiesis mediated by the
HTKL-HTK signaling pathway.
Volume 89,
Issue 8,
pp. 2757-2765,
04/15/1997
Copyright © 1997 by The American Society of Hematology
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