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OX40 expressed on fresh leukemic cells from adult T-cell leukemia patients
mediates cell adhesion to vascular endothelial cells: implication for the
possible involvement of OX40 in leukemic cell infiltration
A Imura, T Hori, K Imada, S Kawamata, Y Tanaka, S Imamura and T Uchiyama
Research Center for Immunodeficiency Virus, Kyoto University, Sakyo, Japan.
We demonstrated previously that OX40 and its ligand, gp34, directly mediate
adhesion of activated normal CD4+ T cells, as well as human T- cell
leukemia virus type I (HTLV-I)-transformed T cells to vascular endothelial
cells. In the present study, we examined expression of OX40 on fresh
leukemic cells from patients with adult T-cell leukemia (ATL) and its
possible involvement in cell adhesion. Flow cytometric analysis showed that
peripheral blood mononuclear cells (PBMC) or lymph node tumor cells from 15
of 17 cases expressed significant levels of OX40 without stimulation. On
the other hand, gp34 was not expressed on these cells, although its
expression is also known to be associated with HTLV- I-infection. In
Western blot analysis, a 50-kD protein band was detected by anti-OX40
monoclonal antibody (MoAb) in two ATL cases examined, as well as
phytohemagglutinin (PHA) blasts and Hut102, an HTLV-I-infected T-cell line,
but not in resting PBMC or Jurkat. Expression of OX40 mRNA was shown by
reverse transcriptase-polymerase chain reaction in all ATL cases tested,
PHA-blasts, and Hut102, but not in resting PBMC or Jurkat. We could not
detect expression of HTLV-I viral mRNA in any of the cases tested. Cell
adhesion assay was performed and in at least three cases, fresh ATL cells
exhibited adhesion to human umbilical vein endothelial cells that could be
considerably inhibited by either anti-OX40 MoAb or anti-gp34 MoAb.
Immunohistochemical staining of skin biopsy specimens indicated that
infiltrating mononuclear cells express OX40 in vivo. Taken together, these
data indicate that leukemic cells from most, but not all, ATL patients
constitutively express OX40, which may play a role in leukemic cell
infiltration in addition to cell adhesion in vivo.
Volume 89,
Issue 8,
pp. 2951-2958,
04/15/1997
Copyright © 1997 by The American Society of Hematology
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