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Lack of expression of Thy-1 (CD90) on acute myeloid leukemia cells with
long-term proliferative ability in vitro and in vivo
A Blair, DE Hogge, LE Ailles, PM Lansdorp and HJ Sutherland
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver Hospital
and Health Sciences Centre, Canada.
Acute myeloid leukaemia (AML) is thought to be maintained by a small
population of leukemic progenitor cells. To define the phenotype of such
cells with long-term proliferative capacity in vitro and in vivo, we have
used the production of leukemic clonogenic cells (CFU) after 2 to 8 weeks
in suspension culture as a measure of these cells in vitro and compared
their phenotype with that of cells capable of engrafting nonobese diabetic
severe combined immune deficient (NOD/SCID) mice. Leukemic blast peripheral
blood cells were evaluated for expression of CD34 and Thy-1 (CD90)
antigens. The majority of AML blast cells at diagnosis lacked expression of
Thy-1. Most primary CFU-blast and the CFU detected at up to 8 weeks from
suspension cultures were CD34+/Thy-1- . AML cells that were capable of
engrafting NOD/SCID mice were also found to have the CD34+/Thy-1-
phenotype. However, significant engraftment was achieved using both
CD34+/Thy-1- and CD34- subfractions from one AML M5 patient. These results
suggest that while heterogeneity exists between individual patients, the
leukemic progenitor cells that are capable of maintaining the disease in
vitro and in vivo differ from normal hematopoietic progenitor cells in
their lack of expression of Thy-1.
Volume 89,
Issue 9,
pp. 3104-3112,
05/01/1997
Copyright © 1997 by The American Society of Hematology
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