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L-selectin-dependent leukocyte adhesion to microvascular but not to
macrovascular endothelial cells of the human coronary system
A Zakrzewicz, M Grafe, D Terbeek, M Bongrazio, W Auch-Schwelk, B Walzog, K Graf, E Fleck, K Ley and P Gaehtgens
Department of Physiology, Freie Universitat Berlin, Germany.
To characterize L-selectin-dependent cell adhesion to human vascular
endothelium, human cardiac microvascular endothelial cells (HCMEC) and
human coronary endothelial cells (HCEC) were isolated from explanted human
hearts. The adhesion behavior of human (NALM-6) and mouse (300.19) pre-B
cells transfected with cDNA encoding for human L- selectin was compared
with that of the respective nontransfected cells in a flow chamber in
vitro. More than 80% of the adhesion to tumor necrosis factor-alpha
(TNF-alpha)-stimulated HCMEC at shear stresses >2 dyne/cm2 was
L-selectin dependent and could be equally well blocked by an
anti-L-selectin antibody or a L-selectin-IgG-chimera. No L-selectin
dependent adhesion to HCEC could be shown. The L-selectin dependent
adhesion to HCMEC was insensitive to neuraminidase, but greatly inhibited
by addition of NaClO3, which inhibits posttranslational sulfation and
remained elevated for at least 24 hours of stimulation. E- selectin
dependent adhesion of HL60 cells to HCMEC was blocked by neuraminidase, but
not by NaClO3 and returned to control levels within 18 hours of HCMEC
stimulation. It is concluded that microvascular, but not macrovascular
endothelial cells express TNF-alpha-inducible sulfated ligand(s) for
L-selectin, which differ from known L-selectin ligands, because sialylation
is not required. The prolonged time course of L-selectin dependent adhesion
suggests a role in sustained leukocyte recruitment into inflammatory sites
in vivo.
Volume 89,
Issue 9,
pp. 3228-3235,
05/01/1997
Copyright © 1997 by The American Society of Hematology
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