|
|
 Previous Article | Table of Contents | Next Article 
Mouse Mast Cells That Possess Segmented/Multi-lobular Nuclei
Michael F. Gurish,
Daniel S. Friend,
Matthew Webster,
Namit Ghildyal,
Christopher F. Nicodemus, and
Richard L. Stevens
From the Departments of Medicine and Pathology, Harvard Medical School; and the Division of Rheumatology, Immunology, and Allergy and the Department of Pathology, Brigham and Women's Hospital, Boston, MA.
Because in humans mast cells and basophils tend to possess nonsegmented and segmented/multi-lobular nuclei, respectively, nuclear morphology has been a major criterion for assessing the lineage of metachromatic cells of hematopoietic origin. Immature metachromatic cells with mono- and multi-lobular nuclei were both obtained when bone marrow cells from BALB/c mice were cultured for 3 weeks in the presence of interleukin-3. Analogous to the indigenous mature mast cells that reside in the peritoneal cavity and skin, both populations of in vitro-derived cells expressed the surface receptor c-kit, the chymase mouse mast cell protease (mMCP) 5, the tryptase mMCP-6, and the exopeptidase carboxypeptidase A (mMC-CPA). Immunogold electron microscopy confirmed the granule location of mMC-CPA and mMCP-6 in both populations of cells, and cytochemical analysis confirmed the presence of chymotryptic enzymes in the granules. Because mature mast cells possessing multi-lobular nuclei also were occasionally found in the skeletal muscle and jejunum of the BALB/c mouse, the V3 mouse mast cell line was used to investigate the developmental relationship of mast cells that have very different nuclear structures. After the adoptive transfer of V3 mast cells into BALB/c mice, v-abl-immortalized mast cells with mono- and multi-lobular nuclei were detected in the lymph nodes and other tissues of the mastocytosis mice that expressed c-kit, mMCP-5, mMCP-6, and mMC-CPA. These studies indicate that mouse mast cells can exhibit varied nuclear profiles. Moreover, the nuclear morphology of this cell type gives no insight as to its protease phenotype or stage of development.
Blood, Vol. 90 No. 1 (July 1), 1997:
pp. 382-390
© 1997 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
D. S. Friend, M. F. Gurish, K. F. Austen, J. Hunt, and R. L. Stevens
Senescent Jejunal Mast Cells and Eosinophils in the Mouse Preferentially Translocate to the Spleen and Draining Lymph Node, Respectively, During the Recovery Phase of Helminth Infection
J. Immunol.,
July 1, 2000;
165(1):
344 - 352.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. S. Kirshenbaum, J. P. Goff, T. Semere, B. Foster, L. M. Scott, and D. D. Metcalfe
Demonstration That Human Mast Cells Arise From a Progenitor Cell Population That Is CD34+, c-kit+, and Expresses Aminopeptidase N (CD13)
Blood,
October 1, 1999;
94(7):
2333 - 2342.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Yuan, M. F. Gurish, D. S. Friend, K. F. Austen, and J. A. Boyce
Cutting Edge: Generation of a Novel Stem Cell Factor-Dependent Mast Cell Progenitor
J. Immunol.,
November 15, 1998;
161(10):
5143 - 5146.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Li, Y. Li, S. W. Reddel, M. Cherrian, D. S. Friend, R. L. Stevens, and S. A. Krilis
Identification of Basophilic Cells that Express Mast Cell Granule Proteases in the Peripheral Blood of Asthma, Allergy, and Drug-Reactive Patients
J. Immunol.,
November 1, 1998;
161(9):
5079 - 5086.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
