|
|
 Previous Article | Table of Contents | Next Article 
Engraftment and Development of Human CD34+-Enriched Cells From Umbilical Cord Blood in NOD/LtSz-scid/scid Mice
Christopher J. Hogan,
Elizabeth J. Shpall,
Oren McNulty,
Ian McNiece,
John E. Dick,
Leonard D. Shultz, and
Gordon Keller
From the Bone Marrow Transplant Program, the Division of Medical Oncology, and the Department of Immunology, University of Colorado Health Sciences Center, Denver; National Jewish Center for Immunology and Respiratory Medicine, Denver, CO; Amgen, Thousand Oaks, CA; the Department of Molecular and Medical Genetics, University of Toronto and the Department of Genetics, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada; and The Jackson Laboratory, Bar Harbor, ME.
Understanding the repopulating characteristics of human hematopoietic stem/progenitor cell fractions is crucial for predicting their performance after transplant into high-risk patients following high-dose therapy. We report that human umbilical cord blood cells, 78% to 100% of which express the hematopoietic progenitor cell surface marker CD34, can consistently engraft, develop, and proliferate in the hematopoietic tissues of sublethally irradiated NOD/LtSz-scid/scid mice. Engraftment and development of CD34+ cells is not dependent on human growth factor support. CD34+ cells home to the mouse bone marrow (BM) that becomes the primary site of human hematopoietic development containing myeloid, lymphoid, erythroid, and CD34+ progenitor populations. Myeloid, and in particular lymphoid cells possessing more mature cell surface markers, comprise the human component of mouse spleen and peripheral blood, indicating that development proceeds from primary hematopoietic sites to the periphery. Repopulation of secondary recipients with human cells by BM from primary recipients demonstrates the maintenance of substantial proliferation capacity of the input precursor population. These data suggest that the cells capable of initiating human cell engraftment (SCID-repopulating cells) are contained in the CD34+ cell fraction, and that this mouse model will be useful for assaying the developmental potential of other rare human hematopoietic cell fractions in vivo.
Blood, Vol. 90 No. 1 (July 1), 1997:
pp. 85-96
© 1997 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
X. Tian, P. S. Woll, J. K. Morris, J. L. Linehan, and D. S. Kaufman
Hematopoietic Engraftment of Human Embryonic Stem Cell-Derived Cells Is Regulated by Recipient Innate Immunity
Stem Cells,
May 1, 2006;
24(5):
1370 - 1380.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Legrand, K. Weijer, and H. Spits
Experimental Models to Study Development and Function of the Human Immune System In Vivo
J. Immunol.,
February 15, 2006;
176(4):
2053 - 2058.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Johnson, N. Shah, A. Panoskaltsis-Mortari, and T. W. LeBien
Murine and Human IL-7 Activate STAT5 and Induce Proliferation of Normal Human Pro-B Cells
J. Immunol.,
December 1, 2005;
175(11):
7325 - 7331.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Reynaud, E. Ravet, M. Titeux, F. Mazurier, L. Renia, A. Dubart-Kupperschmitt, P.-H. Romeo, and F. Pflumio
SCL/TAL1 expression level regulates human hematopoietic stem cell self-renewal and engraftment
Blood,
October 1, 2005;
106(7):
2318 - 2328.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. D. Cravens, M. W. Melkus, A. Padgett-Thomas, M. Islas-Ohlmayer, M. del P. Martin, and J. V. Garcia
Development and Activation of Human Dendritic Cells In Vivo in a Xenograft Model of Human Hematopoiesis
Stem Cells,
February 1, 2005;
23(2):
264 - 278.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Samira, C. Ferrand, A. Peled, A. Nagler, Y. Tovbin, H. Ben-Hur, N. Taylor, A. Globerson, and T. Lapidot
Tumor Necrosis Factor Promotes Human T-Cell Development in Nonobese Diabetic/Severe Combined Immunodeficient Mice
Stem Cells,
November 1, 2004;
22(6):
1085 - 1100.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Bruno, M. Gunetti, L. Gammaitoni, E. Perissinotto, L. Caione, F. Sanavio, F. Fagioli, M. Aglietta, and W. Piacibello
Fast But Durable Megakaryocyte Repopulation and Platelet Production in NOD/SCID Mice Transplanted with Ex-Vivo Expanded Human Cord Blood CD34+ Cells
Stem Cells,
March 1, 2004;
22(2):
135 - 143.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-Y. Huang, H.-F. Tien, F.-H. Su, and S.-M. Hsu
Nonirradiated NOD/SCID-Human Chimeric Animal Model for Primary Human Multiple Myeloma: A Potential in Vivo Culture System
Am. J. Pathol.,
February 1, 2004;
164(2):
747 - 756.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Horn, B. M. Thomasson, B. L. Wood, R. G. Andrews, J. C. Morris, and H.-P. Kiem
Distinct hematopoietic stem/progenitor cell populations are responsible for repopulating NOD/SCID mice compared with nonhuman primates
Blood,
December 15, 2003;
102(13):
4329 - 4335.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. S. Grewal, J. N. Barker, S. M. Davies, and J. E. Wagner
Unrelated donor hematopoietic cell transplantation: marrow or umbilical cord blood?
Blood,
June 1, 2003;
101(11):
4233 - 4244.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Reynaud, N. Lefort, E. Manie, L. Coulombel, and Y. Levy
In vitro identification of human pro-B cells that give rise to macrophages, natural killer cells, and T cells
Blood,
June 1, 2003;
101(11):
4313 - 4321.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yahata, K. Ando, T. Sato, H. Miyatake, Y. Nakamura, Y. Muguruma, S. Kato, and T. Hotta
A highly sensitive strategy for SCID-repopulating cell assay by direct injection of primitive human hematopoietic cells into NOD/SCID mice bone marrow
Blood,
April 15, 2003;
101(8):
2905 - 2913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G R Kolar and J D Capra
The NOD/SCID chimeric mouse model of human B cell development: studies on the VH4 family immunoglobulin repertoire and implications for SLE
Lupus,
March 1, 2003;
12(3):
158 - 162.
[Abstract]
[PDF]
|
|
|
|
|
|
|
A. Nitsche, I. Junghahn, S. Thulke, J. Aumann, A. Radonic, I. Fichtner, and W. Siegert
Interleukin-3 Promotes Proliferation and Differentiation of Human Hematopoietic Stem Cells but Reduces Their Repopulation Potential in NOD/SCID Mice
Stem Cells,
March 1, 2003;
21(2):
236 - 244.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cashman, B. Dykstra, I. Clark-Lewis, A. Eaves, and C. Eaves
Changes in the Proliferative Activity of Human Hematopoietic Stem Cells in NOD/SCID Mice and Enhancement of Their Transplantability after In Vivo Treatment with Cell Cycle Inhibitors
J. Exp. Med.,
November 4, 2002;
196(9):
1141 - 1150.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Saito, Y. Kametani, K. Hozumi, N. Mochida, K. Ando, M. Ito, T. Nomura, Y. Tokuda, H. Makuuchi, T. Tajima, et al.
The in vivo development of human T cells from CD34+ cells in the murine thymic environment
Int. Immunol.,
October 1, 2002;
14(10):
1113 - 1124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. C. Josephson, G. Vassilopoulos, G. D. Trobridge, G. V. Priestley, B. L. Wood, T. Papayannopoulou, and D. W. Russell
Transduction of human NOD/SCID-repopulating cells with both lymphoid and myeloid potential by foamy virus vectors
PNAS,
June 11, 2002;
99(12):
8295 - 8300.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. C. C. Kerre, G. De Smet, M. De Smedt, A. Zippelius, M. J. Pittet, A. W. Langerak, J. De Bosscher, F. Offner, B. Vandekerckhove, and J. Plum
Adapted NOD/SCID model supports development of phenotypically and functionally mature T cells from human umbilical cord blood CD34+ cells
Blood,
March 1, 2002;
99(5):
1620 - 1626.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Bazin, E. Aubin, L. Boyer, I. St-Amour, C. Roberge, and R. Lemieux
Functional in vivo characterization of human monoclonal anti-D in NOD-scid mice
Blood,
February 15, 2002;
99(4):
1267 - 1272.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Hogan, E. J. Shpall, and G. Keller
Differential long-term and multilineage engraftment potential from subfractions of human CD34+ cord blood cells transplanted into NOD/SCID mice
PNAS,
January 8, 2002;
99(1):
413 - 418.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Tonnelle, F. Bardin, C. Maroc, A.-M. Imbert, F. Campa, A. Dalloul, C. Schmitt, and C. Chabannon
Forced expression of the Ikaros 6 isoform in human placental blood CD34+ cells impairs their ability to differentiate toward the B-lymphoid lineage
Blood,
November 1, 2001;
98(9):
2673 - 2680.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. C. C. Kerre, G. De Smet, M. De Smedt, F. Offner, J. De Bosscher, J. Plum, and B. Vandekerckhove
Both CD34+38+ and CD34+38- Cells Home Specifically to the Bone Marrow of NOD/LtSZ scid/scid Mice but Show Different Kinetics in Expansion
J. Immunol.,
October 1, 2001;
167(7):
3692 - 3698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. I. D. Rossi, K. L. Medina, K. Garrett, G. Kolar, P. C. Comp, L. D. Shultz, J. D. Capra, P. Wilson, A. Schipul, and P. W. Kincade
Relatively Normal Human Lymphopoiesis but Rapid Turnover of Newly Formed B Cells in Transplanted Nonobese Diabetic/SCID Mice
J. Immunol.,
September 15, 2001;
167(6):
3033 - 3042.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. P. Dialynas, L.-e. Shao, G. F. Billman, and J. Yu
Engraftment of Human T-Cell Acute Lymphoblastic Leukemia in Immunodeficient NOD/SCID Mice Which Have Been Preconditioned by Injection of Human Cord Blood
Stem Cells,
September 1, 2001;
19(5):
443 - 452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. P. Dialynas, M.-J. Lee, D. P. Gold, L.-e. Shao, A. L. Yu, M. J. Borowitz, and J. Yu
Preconditioning with fetal cord blood facilitates engraftment of primary childhood T-cell acute lymphoblastic leukemia in immunodeficient mice
Blood,
May 15, 2001;
97(10):
3218 - 3225.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Debili, C. Robin, V. Schiavon, R. Letestu, F. Pflumio, M.-T. Mitjavila-Garcia, L. Coulombel, and W. Vainchenker
Different expression of CD41 on human lymphoid and myeloid progenitors from adults and neonates
Blood,
April 1, 2001;
97(7):
2023 - 2030.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. E. Perez, H. M. Rinder, C. Wang, J. B. Tracey, N. Maun, and D. S. Krause
Xenotransplantation of immunodeficient mice with mobilized human blood CD34+ cells provides an in vivo model for human megakaryocytopoiesis and platelet production
Blood,
March 15, 2001;
97(6):
1635 - 1643.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Rosu-Myles, L. Gallacher, B. Murdoch, D. A. Hess, M. Keeney, D. Kelvin, L. Dale, S. S. G. Ferguson, D. Wu, F. Fellows, et al.
The human hematopoietic stem cell compartment is heterogeneous for CXCR4 expression
PNAS,
December 19, 2000;
97(26):
14626 - 14631.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ueda, H. Yoshino, K. Kobayashi, M. Kawahata, Y. Ebihara, M. Ito, S. Asano, T. Nakahata, and K. Tsuji
Hematopoietic Repopulating Ability of Cord Blood CD34+ Cells in NOD/Shi-scid Mice
Stem Cells,
May 1, 2000;
18(3):
204 - 213.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
L. Gallacher, B. Murdoch, D. M. Wu, F. N. Karanu, M. Keeney, and M. Bhatia
Isolation and characterization of human CD34-Lin- and CD34+Lin- hematopoietic stem cells using cell surface markers AC133 and CD7
Blood,
May 1, 2000;
95(9):
2813 - 2820.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. D. Shultz, P. A. Lang, S. W. Christianson, B. Gott, B. Lyons, S. Umeda, E. Leiter, R. Hesselton, E. J. Wagar, J. H. Leif, et al.
NOD/LtSz-Rag1null Mice: An Immunodeficient and Radioresistant Model for Engraftment of Human Hematolymphoid Cells, HIV Infection, and Adoptive Transfer of NOD Mouse Diabetogenic T Cells
J. Immunol.,
March 1, 2000;
164(5):
2496 - 2507.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Schiedlmeier, K. Kuhlcke, H. G. Eckert, C. Baum, W. J. Zeller, and S. Fruehauf
Quantitative assessment of retroviral transfer of the human multidrug resistance 1 gene to human mobilized peripheral blood progenitor cells engrafted in nonobese diabetic/severe combined immunodeficient mice
Blood,
February 15, 2000;
95(4):
1237 - 1248.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. R. Davis, J. Yannariello-Brown, N. L. Prokopishyn, Z. Luo, M. R. Smith, J. Wang, N. D. V. Carsrud, and D. B. Brown
Glass needle-mediated microinjection of macromolecules and transgenes into primary human blood stem/progenitor cells
Blood,
January 15, 2000;
95(2):
437 - 444.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Gutiérrez-Rodríguez, E. Reyes-Maldonado, and H. Mayani
Characterization of the Adherent Cells Developed in Dexter-Type Long-Term Cultures from Human Umbilical Cord Blood
Stem Cells,
January 1, 2000;
18(1):
46 - 52.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Nakamura, K. Ando, J. Chargui, H. Kawada, T. Sato, T. Tsuji, T. Hotta, and S. Kato
Ex Vivo Generation of CD34+ Cells From CD34- Hematopoietic Cells
Blood,
December 15, 1999;
94(12):
4053 - 4059.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Crisa, V. Cirulli, K. A. Smith, M. H. Ellisman, B. E. Torbett, and D. R. Salomon
Human Cord Blood Progenitors Sustain Thymic T-Cell Development and a Novel Form of Angiogenesis
Blood,
December 1, 1999;
94(11):
3928 - 3940.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. M. Novelli, M. Ramírez, W. Leung, and C. I. Civin
Human Hematopoietic Stem/Progenitor Cells Generate CD5+ B Lymphoid Cells in NOD/SCID Mice
Stem Cells,
September 1, 1999;
17(5):
242 - 252.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
W. Piacibello, F. Sanavio, A. Severino, A. Dane, L. Gammaitoni, F. Fagioli, E. Perissinotto, G. Cavalloni, O. Kollet, T. Lapidot, et al.
Engraftment in Nonobese Diabetic Severe Combined Immunodeficient Mice of Human CD34+ Cord Blood Cells After Ex Vivo Expansion: Evidence for the Amplification and Self-Renewal of Repopulating Stem Cells
Blood,
June 1, 1999;
93(11):
3736 - 3749.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Robin, F. Pflumio, W. Vainchenker, and L. Coulombel
Identification of Lymphomyeloid Primitive Progenitor Cells in Fresh Human Cord Blood and in the Marrow of Nonobese Diabetic-Severe Combined Immunodeficient (NOD-SCID) Mice Transplanted with Human CD34+ Cord Blood Cells
J. Exp. Med.,
May 17, 1999;
189(10):
1601 - 1610.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Guenechea, J.C. Segovia, B. Albella, M. Lamana, M. Ramirez, C. Regidor, M.N. Fernandez, and J.A. Bueren
Delayed Engraftment of Nonobese Diabetic/Severe Combined Immunodeficient Mice Transplanted With Ex Vivo-Expanded Human CD34+ Cord Blood Cells
Blood,
February 1, 1999;
93(3):
1097 - 1105.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Miyoshi, K. A. Smith, D. E. Mosier, I. M. Verma, and B. E. Torbett
Transduction of Human CD34+ Cells That Mediate Long-Term Engraftment of NOD/SCID Mice by HIV Vectors
Science,
January 29, 1999;
283(5402):
682 - 686.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. D. Cashman and C. J. Eaves
Human Growth Factor-Enhanced Regeneration of Transplantable Human Hematopoietic Stem Cells in Nonobese Diabetic/Severe Combined Immunodeficient Mice
Blood,
January 15, 1999;
93(2):
481 - 487.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Schilz, G. Brouns, O. G. Ottmann, D. Hoelzer, A. A. Fauser, A. J. Thrasher, and M. Grez
High Efficiency Gene Transfer to Human Hematopoietic SCID-Repopulating Cells Under Serum-Free Conditions
Blood,
November 1, 1998;
92(9):
3163 - 3171.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C.M. van der Loo, H. Hanenberg, R. J. Cooper, F.-Y. Luo, E. N. Lazaridis, and D. A. Williams
Nonobese Diabetic/Severe Combined Immunodeficiency (NOD/SCID) Mouse as a Model System to Study the Engraftment and Mobilization of Human Peripheral Blood Stem Cells
Blood,
October 1, 1998;
92(7):
2556 - 2570.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Kapp, M. Bhatia, D. Bonnet, B. Murdoch, and J. E. Dick
Treatment of Non-Obese Diabetic (NOD)/Severe-Combined Immunodeficient Mice (SCID) With flt3 Ligand and Interleukin-7 Impairs the B-Lineage Commitment of Repopulating Cells After Transplantation of Human Hematopoietic Cells
Blood,
September 15, 1998;
92(6):
2024 - 2031.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Mayani and P. M. Lansdorp
Biology of Human Umbilical Cord Blood-Derived Hematopoietic Stem/Progenitor Cells
Stem Cells,
May 1, 1998;
16(3):
153 - 165.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. L. Greiner, R. A. Hesselton, and L. D. Shultz
SCID Mouse Models of Human Stem Cell Engraftment
Stem Cells,
May 1, 1998;
16(3):
166 - 177.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. M.A. Verstegen, P. B. van Hennik, W. Terpstra, C. van den Bos, J. J. Wielenga, N. van Rooijen, R. E. Ploemacher, G. Wagemaker, and A. W. Wognum
Transplantation of Human Umbilical Cord Blood Cells in Macrophage-Depleted SCID Mice: Evidence for Accessory Cell Involvement in Expansion of Immature CD34+CD38- Cells
Blood,
March 15, 1998;
91(6):
1966 - 1976.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. D. Lewis, L. A. McDiarmid, L. M. Samels, L. Bik To, and T. P. Hughes
Establishment of a Reproducible Model of Chronic-Phase Chronic Myeloid Leukemia in NOD/SCID Mice Using Blood-Derived Mononuclear or CD34+ Cells
Blood,
January 15, 1998;
91(2):
630 - 640.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
